Zepeda-Cisneros C.S.,Moscafrut Program |
Hernandez J.S.M.,Moscafrut Program |
Garcia-Martinez V.,Moscafrut Program |
Ibanez-Palacios J.,Moscafrut Program |
And 2 more authors.
BMC Genetics | Year: 2014
Background: Anastrepha ludens is among the pests that have a major impact on México's economy because it attacks fruits as citrus and mangoes. The Mexican Federal government uses integrated pest management to control A. ludens through the Programa Nacional Moscas de la Fruta [National Fruit Fly Program, SAGARPASENASICA]. One of the main components of this program is the sterile insect technique (SIT), which is used to control field populations of the pest by releasing sterile flies. Results: To increase the efficiency of this technique, we have developed a genetic sexing strain (GSS) in which the sexing mechanism is based on a pupal colour dimorphism (brown-black) and is the result of a reciprocal translocation between the Y chromosome and the autosome bearing the black pupae (bp) locus. Ten strains producing wild-type (brown pupae) males and mutant (black pupae) females were isolated. Subsequent evaluations for several generations were performed in most of these strains. The translocation strain named Tapachula-7 showed minimal effect on survival and the best genetic stability of all ten strains. Genetic and cytogenetic analyses were performed using mitotic and polytene chromosomes and we succeeded to characterize the chromosomal structure of this reciprocal translocation and map the autosome breakpoint, despite the fact that the Y chromosome is not visible in polytene nuclei following standard staining. Conclusions: We show that mitotic and polytene chromosomes can be used in cytogenetic analyses towards the development of genetic control methods in this pest species. The present work is the first report of the construction of GSS of Anastrepha ludens, with potential use in a future Moscafrut operational program. © 2014 Zepeda-Cisneros et al.
Orozco D.,Moscafrut Program |
Salvador Meza J.,Moscafrut Program |
Zepeda S.,Moscafrut Program |
Solis E.,Moscafrut Program |
Luis Quintero-Fong J.,Moscafrut Program
Journal of Economic Entomology | Year: 2013
A new genetic sexing strain of the Mexican fruit fly, Anastrepha ludens (Loew), was evaluated in tests of sexual behavior to determine its possible application using the sterile insect technique. Tests in field cages measuring time to sexual maturity, compatibility with wild flies, and competitiveness were compared between the genetic sexing strain, Tapachula-7, and the mass-reared standard bisexual strain. The results indicated that the onset of sexual maturity was similar for both laboratory strains. Males from the Tapachula-7 strain do not differ from the standard bisexual strain in compatibility and competitiveness with wild insects. The results indicate that the release of Tapachula-7 males in the field would be viable in programs that use the sterile insect technique for the control of the Mexican fruit fly. © 2013 Entomological Society of America.
Orozco D.,Moscafrut Program |
Hernandez M.R.,Moscafrut Program |
Meza J.S.,Moscafrut Program |
Quintero J.L.,Moscafrut Program
Journal of Applied Entomology | Year: 2013
The release of sterile males to suppress the Mexican fruit fly Anastrepha ludens (Loew) population was simulated in field cages to compare the sexual performance of sterile males with wild females in the presence and absence of sterile females. Using different release ratios of sterile and wild individuals, this study showed that the presence of sterile females negatively affected the sexual performance of sterile males in mating with wild females. We found that the sterility induced was proportional to the magnitude of the sterile/fertile ratio. These results indicate that the release of sterile males without sterile females can significantly improve the efficiency of the sterile insect technique for the control of A. ludens. © 2012 Blackwell Verlag, GmbH.