Haynes L.,Kraft Foods Inc. |
Schuenzel M.,Morris College
Journal of Thermal Analysis and Calorimetry | Year: 2011
Thermal characteristics of refined wheat flour blend made with 90% Ohio soft red winter wheat and 10% Canadian Hard Red Winter Wheat are explored using a series of modulated differential scanning calorimeter conditions. Influences of pan type, period, amplitude of the thermal modulation, and underlying thermal heating rate on flour starch thermal transitions are presented. Wheat flour was blend 1:1 with water, and then heated at various heating rates while the amplitude and period of the instantaneous heating rate was modulated between ±0.5 and ±1.0 °C amplitude and 60 s to 80 s period. Study shows faster heating rates favor increased total heat flow and results in proportional increases in both reversing and non-reversing thermokinetic events of recrystallization. Slower overall heating rates (e.g., 2.5 °C min -1) produced better resolution of thermal events related to preexisting structural phases, but allowed more time for creation of new events such as recrystallization, annealing. © 2011 Akadémiai Kiadó, Budapest, Hungary.
Ponikvar-Svet M.,Jozef Stefan Institute |
Zeiger D.N.,American Journal Experts |
Keating L.R.,Morris College |
Liebman J.F.,University of Maryland Baltimore County
Structural Chemistry | Year: 2013
The contents of issues 1 and 2 for the calendar year 2013 are summarized in the current review of the journal Structural Chemistry. In addition, a brief thermochemical commentary is added to the summary of each paper. © 2013 Springer Science+Business Media New York.
Gavrilov A.V.,Kazan State Technological University |
Yaw E.J.,Morris College
2013 International Conference on Interactive Collaborative Learning, ICL 2013 | Year: 2013
Globalization processes influence on modern educational system of Russian Federation is great. It becomes an important part of social development innovative model. Cluster systems combining educational institutions, science, enterprises and goods market is the promoter of this process. © 2013 IEEE.
Yao C.H.,Palmer Laboratory of Cell and Molecular Biology |
Zhang P.,Yale University |
Zhang L.,Palmer Laboratory of Cell and Molecular Biology |
Zhang L.,Morris College
Biochemistry and Cell Biology | Year: 2012
The calmodulin-dependent kinase (CaMK) family has been recently recognized to participate in the regulation of osteoclastogenesis. However, there are some controversial reports regarding the mRNA expression patterns of CaMKs during osteoclastogenesis, although the protein expression pattern of most CaMKs during osteoclastogenesis have not been studied. In the present study, we attempted to address this issue by using a mouse bone marrow monocyte model and parallel Western blotting and quantitative real-time PCR. Our results revealed some interesting expression patterns of CaMKs during the process. Among all CaMKs examined, only CaMKIId exhibited consistent expression patterns between its mRNA and protein with both rising remarkably during osteoclastogenesis. CaMKIV protein was not detectable during the first three days of cell culture, but it rose on Day 5. The CaMK inhibitor, KN93, subdued osteoclastogenesis during the first three days of cell culture, a time when CaMKIV was absent while other KN93-sensitive CaMKs presented. In addition, KN93 was found to inhibit the expression of some early receptor activator of NF-kB (RANK) signaling intermediates (extracellular signal-regulated kinase (ERK) and Akt) in the non-differentiated mouse bone marrow monocytes. Collectively, these data reveal differential expression patterns of KN93-sensitive CaMK proteins and their mRNAs during osteoclastogenesis, supporting a CaMKII-RANK signaling interaction in the regulation of early osteoclastogenesis. © 2012 Published by NRC Research Press.
Gianella P.,Morris College |
Snapp E.L.,York College |
Levy M.,Morris College
Biotechnology and Bioengineering | Year: 2016
We have developed a generalized in vitro compartmentalization-based bead display selection strategy that allows for the identification of enzymes that can perform ligation reactions. Although a number of methods have been developed to evolve such enzymes, many of them are limited in library size (106-107), do not select for enzymes using a scheme that allows for multiple turnover, or only work on enzymes specific to nucleic acids. This approach is amenable to screening libraries of up to 1012 protein variants by allowing beads to be overloaded with up to 104 unique mutants. Using this approach we isolated a variant of sortase A from Staphylococcus aureus that shows a 114-fold enhancement in kcat/KM in the absence of calcium compared to the wild-type and improved resistance to the inhibitory effects of cell lysates. Unlike the wild-type protein, the newly selected variant shows intracellular activity in the cytoplasm of eukaryotic cells where it may prove useful for intracellular labeling or synthetic biological applications. © 2016 Wiley Periodicals, Inc.
Keene K.,Morris College
Chiropractic Journal of Australia | Year: 2011
Objective: Chiropractors frequently treat lumbar disc pathology in practice, but typically in adult patients. This case involves a 16-year-old gridiron football player diagnosed with an L5 disc herniation associated with sacroiliac joint dysfunction, spina bifida occulta and knife-clasp syndrome. Clinical Features: A 16-year-old high school football player with a history of chronic low back pain and multiple traumas sought care for his chronic low back pain. Radiographic examination showed a non-union at S1, and MRI examination showed a disc herniation at L5-S1. Diagnosis was lumbar disc herniation with spina bifida occulta and knife-clasp syndrome. Intervention and Outcome: The patient was managed with chiropractic care over a period of 24 weeks. Flexion-distraction manipulation was utilized, along with physical therapy modalities and core-strengthening exercises. On re-evaluation his condition was significantly improved. Because of this diagnosis, he and his parents questioned the safety of sports participation and whether or not he would qualify for a military career; we therefore explored sports participation and career advice. Conclusion: This case describes chiropractic management of an adolescent with a constellation of conditions not usually seen in this age group. Information is provided on how to advise patients about the possibility of serious injury during sports participation as well as potential military career choices based on this diagnosis.
Rollinson S.W.,Morris College
American Biology Teacher | Year: 2012
The growth of a pine tree is examined by preparing "tree cookies" (cross-sectional disks) between whorls of branches. The use of Christmas trees allows the tree cookies to be obtained with inexpensive, commonly available tools. Students use the tree cookies to investigate the annual growth of the tree and how it corresponds to the number of whorls on the tree. Heightdiameter and heightage relationships are also investigated. While aimed at high school classes, the procedure can be adapted for younger students. © 2012 by National Association of Biology Teachers. All rights reserved.
Dybas J.M.,Morris College |
Fiser A.,Morris College
Proteins: Structure, Function and Bioinformatics | Year: 2016
Structure conservation, functional similarities, and homologous relationships that exist across diverse protein topologies suggest that some regions of the protein fold universe are continuous. However, the current structure classification systems are based on hierarchical organizations, which cannot accommodate structural relationships that span fold definitions. Here, we describe a novel, super-secondary-structure motif-based, topology-independent structure comparison method (SmotifCOMP) that is able to quantitatively identify structural relationships between disparate topologies. The basis of SmotifCOMP is a systematically defined super-secondary-structure motif library whose representative geometries are shown to be saturated in the Protein Data Bank and exhibit a unique distribution within the known folds. SmotifCOMP offers a robust and quantitative technique to compare domains that adopt different topologies since the method does not rely on a global superposition. SmotifCOMP is used to perform an exhaustive comparison of the known folds and the identified relationships are used to produce a nonhierarchical representation of the fold space that reflects the notion of a continuous and connected fold universe. The current work offers insight into previously hypothesized evolutionary relationships between disparate folds and provides a resource for exploring novel ones. © 2016 Wiley Periodicals, Inc.
Agency: NSF | Branch: Standard Grant | Program: | Phase: ADVANCED TECH EDUCATION PROG | Award Amount: 200.00K | Year: 2014
The Pathways of Applied Career Experience (PACE) Science Program provides opportunities for students enrolled in the Biotechnology and Chemical Technology programs to complete internships and other applied industry experiences to supplement student learning. Partnerships with Industry Alliance Committee members are increasing student internship offerings and utilizing industry feedback to align key courses with industry needs and therefore establishing a feedback mechanism that continually improves student outcomes. Increasing internship participation rates for all students and aligning key courses with industry needs contributes highly trained entry-level employees into the local technology economy. The PACE program addresses students in need of remediation and improves the retention of all students by using Process Oriented Guided Inquiry Learning (POGIL) activities in selected courses. POGIL activities engage students in higher levels of thinking and collaborative group learning. It is a proven pedagogy that promotes many skills employers seek: teamwork, communication, problem solving, strong work ethic, analytical approach, taking initiative, critical thinking and time management. By implementing POGIL, technology students are more active in their learning and are better equipped with useful skills to enter the workforce. The PACE Science Program allows CCM to meet the needs of the STEM economy and the alliance with local industries demonstrates the success of the project by the number of prepared technicians produced and successfully employed.
Lipton R.B.,Morris College
CONTINUUM Lifelong Learning in Neurology | Year: 2015
Purpose of the Review: This review focuses on patients withmigrainewho frequently use acute medications. Recent Findings: Chronic migraine and medication-overuse headache are common in the general population and often coexist. According to new diagnostic criteria, both diagnoses can be made for an individual patient. Evidence is slowly emerging on the most appropriate management approach for both disorders. Summary: Although the relationship of the primary headache disorder and the pattern of overuse varies, medication-overuse headache is a secondary disorder attributable to the overuse of acute medications. While distinguishing chronic migraine and medication-overuse headache may not always be possible, treatment approaches are similar for the two disorders. © 2015, American Academy of Neurology.