Yao C.H.,Palmer Laboratory of Cell and Molecular Biology |
Zhang P.,Yale University |
Zhang L.,Palmer Laboratory of Cell and Molecular Biology |
Zhang L.,Morris College
Biochemistry and Cell Biology | Year: 2012
The calmodulin-dependent kinase (CaMK) family has been recently recognized to participate in the regulation of osteoclastogenesis. However, there are some controversial reports regarding the mRNA expression patterns of CaMKs during osteoclastogenesis, although the protein expression pattern of most CaMKs during osteoclastogenesis have not been studied. In the present study, we attempted to address this issue by using a mouse bone marrow monocyte model and parallel Western blotting and quantitative real-time PCR. Our results revealed some interesting expression patterns of CaMKs during the process. Among all CaMKs examined, only CaMKIId exhibited consistent expression patterns between its mRNA and protein with both rising remarkably during osteoclastogenesis. CaMKIV protein was not detectable during the first three days of cell culture, but it rose on Day 5. The CaMK inhibitor, KN93, subdued osteoclastogenesis during the first three days of cell culture, a time when CaMKIV was absent while other KN93-sensitive CaMKs presented. In addition, KN93 was found to inhibit the expression of some early receptor activator of NF-kB (RANK) signaling intermediates (extracellular signal-regulated kinase (ERK) and Akt) in the non-differentiated mouse bone marrow monocytes. Collectively, these data reveal differential expression patterns of KN93-sensitive CaMK proteins and their mRNAs during osteoclastogenesis, supporting a CaMKII-RANK signaling interaction in the regulation of early osteoclastogenesis. © 2012 Published by NRC Research Press.
Keene K.,Morris College
Chiropractic Journal of Australia | Year: 2011
Objective: Chiropractors frequently treat lumbar disc pathology in practice, but typically in adult patients. This case involves a 16-year-old gridiron football player diagnosed with an L5 disc herniation associated with sacroiliac joint dysfunction, spina bifida occulta and knife-clasp syndrome. Clinical Features: A 16-year-old high school football player with a history of chronic low back pain and multiple traumas sought care for his chronic low back pain. Radiographic examination showed a non-union at S1, and MRI examination showed a disc herniation at L5-S1. Diagnosis was lumbar disc herniation with spina bifida occulta and knife-clasp syndrome. Intervention and Outcome: The patient was managed with chiropractic care over a period of 24 weeks. Flexion-distraction manipulation was utilized, along with physical therapy modalities and core-strengthening exercises. On re-evaluation his condition was significantly improved. Because of this diagnosis, he and his parents questioned the safety of sports participation and whether or not he would qualify for a military career; we therefore explored sports participation and career advice. Conclusion: This case describes chiropractic management of an adolescent with a constellation of conditions not usually seen in this age group. Information is provided on how to advise patients about the possibility of serious injury during sports participation as well as potential military career choices based on this diagnosis.
Gavrilov A.V.,Kazan State Technological University |
Yaw E.J.,Morris College
2013 International Conference on Interactive Collaborative Learning, ICL 2013 | Year: 2013
Globalization processes influence on modern educational system of Russian Federation is great. It becomes an important part of social development innovative model. Cluster systems combining educational institutions, science, enterprises and goods market is the promoter of this process. © 2013 IEEE.
Ponikvar-Svet M.,Jozef Stefan Institute |
Zeiger D.N.,American Journal Experts |
Keating L.R.,Morris College |
Liebman J.F.,University of Maryland Baltimore County
Structural Chemistry | Year: 2013
The contents of issues 1 and 2 for the calendar year 2013 are summarized in the current review of the journal Structural Chemistry. In addition, a brief thermochemical commentary is added to the summary of each paper. © 2013 Springer Science+Business Media New York.
Rollinson S.W.,Morris College
American Biology Teacher | Year: 2012
The growth of a pine tree is examined by preparing "tree cookies" (cross-sectional disks) between whorls of branches. The use of Christmas trees allows the tree cookies to be obtained with inexpensive, commonly available tools. Students use the tree cookies to investigate the annual growth of the tree and how it corresponds to the number of whorls on the tree. Heightdiameter and heightage relationships are also investigated. While aimed at high school classes, the procedure can be adapted for younger students. © 2012 by National Association of Biology Teachers. All rights reserved.