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Bochum-Hordel, Germany

Asran A.S.,Martin Luther University of Halle Wittenberg | Asran A.S.,National Research Center of Egypt | Sanger T.,University of Duisburg - Essen | Laub M.,Morphoplant GmbH | And 3 more authors.
Biomedizinische Technik | Year: 2014

The objective of the present work is to fabricate and develop growth factor-PDLLA nano-composites by electrospinning into 2D fleece or 3D tubular scaffolds that can mimic the nature and function of the native extracellular matrix of bone tissue or artificial blood vessels. The bioactive growth factors 125I-rhVEGF165, 125IrhBMP- 2 and the fibrous protein collagen were incorporated directly into the nascent PDLLA fiber during the electrospinning process. The morphology and distribution of the bioactive molecules in the electrospun fibers were investigated using TEM. The micro mechanical deformation was studied in tension tests using ESEM. The electrospun PDLLA nanofibers loaded with rhVEGF165 and collagen showed a ductile behavior with necking and cold drawing deformation on stretching. The incorporation of rhVEGF165, rhBMP-2 and collagen molecules into the scaffolds of PDLLA nanofibers could enhance cell attachment, reduce the scaffolds lifetime and promote angiogenesis and osteogenesis process, and thereby induce bone formation. © 2014 by Walter de Gruyter Berlin Boston. Source


Luers S.,Morphoplant GmbH | Jennissen H.P.,Universitatsklinikum Essen
Biomedizinische Technik | Year: 2014

Two methods of contact angle measurement are applied on screw type dental implants composed of titanium with different surfaces: The standard Wilhelmy method (dynamic measurement) and an extended sessile drop method (static measurement), where droplets with a volume in the picoliter range are generated. Both methods lead to comparable but in detail different results. © 2014 by Walter de Gruyter Berlin Boston. Source


Zurlinden K.,Morphoplant GmbH | Laub M.,Morphoplant GmbH | Dohle D.-S.,University of Duisburg - Essen | Jennissen H.P.,University of Duisburg - Essen
Biomedizinische Technik | Year: 2012

Angiogenesis and osteogenesis are closely related processes sharing some essential mediators like VEGF (Vascular Endothelial Growth Factor) and BMP-2 (Bone Morphogenetic Protein-2). As vascularization is crucial to bone formation biofunctionalization of bone replacement materials with both of these proteins may result in enhanced bone formation. Here we will present immobilization of self prepared biologically active BMP-2 and VEGF on different bone replacement materials and their controlled release. The amount of bound protein can be steered as a function of protein concentration in the incubation solution. Desorption behaviour of proteins differs strongly depending on the type of bone replacement material used and protein ranging from 0.1-2 days for the burst phase and from 17-115 days for the sustained release phase. A combined loading and release of the growth factors is now planned. © 2012 by Walter de Gruyter Berlin Boston. Source


Sanger T.,University of Duisburg - Essen | Sh. Asran A.,Martin Luther University of Halle Wittenberg | Laub M.,Morphoplant GmbH | Michler G.H.,Martin Luther University of Halle Wittenberg | Jennissen H.P.,University of Duisburg - Essen
Biomedizinische Technik | Year: 2014

Electro spun PDLLA-nanofibers were functionalized with 125I-rhBMP-2 and 125I-rhVEGF165, either by protein-adsorption to, or by incorporation into electrospun PDLLA-nanofibers. Both, adsorbed and incorporated growth factors could be visualized via autoradiography. By fitting the release data according the two phase exponential decay, release rates as well as release-half-lives could be calculated mathematically. Moreover different release rates could be observed, suitable to design multimodal hybrid materials. Loading the PDLLA-nanofibers with rhBMP-2 and/or rhVEGF165 would be of benefit in bone regeneration. © 2014 by Walter de Gruyter Berlin Boston. Source

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