Mohammed S.I.,North Maharashtra University |
Chopda M.Z.,Moolji Jaitha College |
Patil R.H.,Science and Commerce College |
Vishwakarma K.S.,North Maharashtra University |
Maheshwari V.L.,North Maharashtra University
Asian Pacific Journal of Tropical Disease | Year: 2016
Objective: To evaluate antidiabetic and antioxidative properties of Coccinia grandis leaf extract in experimental diabetic rats and to study the co-relation of the two in vivo. Methods: Ethanolic extract, showing the highest activity in in vitro experiments, was prepared in saline and given orally to streptozotocin induced albino Wistar diabetic rats for 21 days. Biochemical parameters, histopathological analyses, liver and muscles glycogen and in vivo antioxidant activity in normal, diabetic control, standard (metformin) and treated animals were determined and compared. Results: Treatment of experimental rats with diabetes induced by streptozotocin by ethanolic leaf extract (500 mg/kg) caused significant (P < 0.001) reduction in blood glucose (312-169 mg/100 mL), increase in body weight (181-210 g) and serum insulin (1.28-3.10 IU/dL). It also maintained lipid profile and liver and kidney functions within normal range compared with diabetic control rats and almost at par with metformin-a standard antidiabetic drug. The oxidative stress induced decline in glutathione and catalase in liver and kidney tissues showed up to 60% recovery as a function of treatment. Histopathology of pancreas showed marked restorative effect in diabetic rats. Conclusions: The results suggest that Coccinia grandis ethanolic leaf extract has strong antidiabetic activity and can be meaningfully utilized in the management of diabetes. © 2016 Asian Pacific Tropical Medicine Press.
Khadse V.R.,Moolji Jaitha College |
Thakur S.,North Maharashtra University |
Patil K.R.,CSIR - National Chemical Laboratory |
Patil P.,North Maharashtra University
Sensors and Actuators, B: Chemical | Year: 2014
We report the fabrication of a highly sensitive and fast humidity sensor based on cerium oxide (CeO2) nanoparticles, which were prepared at low cost via a simple non-isothermal precipitation method. The as-prepared CeO2 nanoparticles were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) and the results indicated the formation of face centered cubic phase of CeO2 with average crystallite size of approximately 10-12 nm. Humidity sensors based on CeO 2 nanoparticles exhibit high and linear response within the whole relative humidity (RH) range of 11-97% at an operating frequency of 60 Hz. The corresponding impedance changes by approximately three orders of magnitude within the entire humidity range from 11% to 97% RH. The response and recovery times are about 2-3 and 9-10 s, respectively, when RH was switched between 11% and 97%. Furthermore, the sensors also show relatively small hysteresis, excellent reproducibility, long term stability and broad range of operation (11-97% RH). The complex impedance spectra of the sensor at different RHs and the equivalent circuit were analyzed to explore the humidity-sensing mechanism. This study demonstrates that the CeO2 nanoparticles prepared by non-precipitation method can be used as the humidity-sensing material for the fabrication of humidity sensors. © 2014 Elsevier B.V.
Patil K.J.,Moolji Jaitha College |
Mahajan R.T.,Technology and Research
International Journal of Pharma and Bio Sciences | Year: 2016
The phenotypic, phylogenetic, geographical diversity and sustainability ability of micro-algae capable of producing enzymes provides a challenge for the accurate determination of biotechnological potential. For production of economically important products like agar, alginates, biodiesel, bio-fuel and medicinally important products algae extensively employed. Although, so lengthily for enzyme production. The modified methods developed to facilitate fast and reliable screening of enzyme production. Experiments carried out on nine macro algae and ten micro-algal strains isolated from Jalgaon region, Maharashtra (India) and two micro-algal strains obtained from BIT Ranchi, an approach found to provide a more reliable measure for enzyme production. Out of 21 algae, 5 strains are positive for lipase, 20 for phosphatase, 06 for amylase, 07 for gelatinase, 05 for urease and 19 for catalase. This is an important escalation for isolation and screening of enzyme production from microalgae.
Patil K.J.,Moolji Jaitha College |
Mahajan R.T.,Technology and Research |
Lautre H.K.,P.A. College |
Patil V.A.,P.A. College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2016
Algae are also very important ecologically because they are the beginning of the food chain for other animals and used in production of many economical valuable products. Whereas, ample of reports are available in morphological studies on fresh water algae throughout the globe. However, enzyme research of algal origin is ephemeral. Hydrolytic enzymes have great reasonable significance owing to their central role in biological processes. In this study, acid phosphatase enzyme characterized from Scenedesmus obliquus fresh water alga isolated province Waghur River located near Skegaon, district Jalgaon, Maharashtra (India). The acid phosphatase is a monomer protein purified by ion-exchange and gel filtration to 6.36 fold with an apparent molecular mass 59.41 kDa on SDS PAGE. Farther, it evidenced by LCMS analysis. It has 56.10 kDa molecular with sequence (K)IINSDNVQEAAR(E), which is partial analogous with the glucose-1-phosphate adenylyl transferase, a small subunit of Arabidopsis thaliana. The purified acid phosphatase has an optimum pH of 5.0, and optimum temperature for the hydrolysis of p-Nitro phenyl phosphate at 50oC and the km and Vmax 0.24 mm and 0.02 μmol min-1mg-1 respectively at the same conditions. The activation energy found to be 32.35 and Q10 value was 1.54 between 40 and 50°C and fairly stable at temperature up to 37°C. The activity of the enzyme enhanced by Triton X, Guaiacol and EDTA and heavy metals Fe3+, Cu2+, and Ca2+. The enzyme was strongly inhibited by organic solvents, SDS, tween 80 and heavy metals Zn2+, Mg2+, Hg2+ and K+. The present article reveals on bio-molecular characterization of acid phosphatase with kinetic studies.
Patil K.J.,P.A. College |
Chopda M.Z.,Moolji Jaitha College |
Mahajan R.T.,Moolji Jaitha College
Indian Journal of Science and Technology | Year: 2011
Industries prefer biocatalysts rather than chemical catalyst. Lipase a biocatalyst is a versatile enzyme that not only hydrolyzes the esters of long chain aliphatic acids form glycerol at oil or water interface but also involved in hydrolysis, transesterification, alcoholysis, and aminolysis. Lipases are widely distributed in microorganisms, plants and animals. Among them microbial lipases are preferred because of easily obtainable. Lipases are used in many fields like food, dairy, detergent, pharmaceutical, agrochemical and oleochemical industries. Based on the data compiled it reveals that the contribution of bacterial lipases is 45%, fungal 21%, animal 18%, plants 11% and algae 3%. This article provides information about comparative account of bacterial, fungal, plant and animal origin lipases along with their biochemical profiles. It also focuses on the need in search of algal lipases. © Indian Society for Education and Environment (iSee).