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Vijayawāda, India

Sreelakshmi A.,Montessori Mahila Kalasala
Biosciences Biotechnology Research Asia | Year: 2013

Naratriptan is a triptan drug. Two simple, sensitive and accurate spectrophotometric methods have been developed for the determination of Naratriptan in pure state and in its pharmaceutical dosage forms. The developed Method A, involves the formation of drug-picric acid complex in chloroform solution and it shows maximum absorption at λ max 400 nm; linearity in the range of 2.5-12.5 μg/mL. Method B is based on the formation of charge transfer complex formation of chloranilic acid with drug. The developed chromogen in Method B exhibits maximum absorption at λ max 525 nm and linearity in the range of 100-500 μg/mL. The results obtained were statistically evaluated and were found to be accurate and reproducible. Source


Sreelakshmi A.,Montessori Mahila Kalasala
Journal of Chemical and Pharmaceutical Sciences | Year: 2010

Ziprasidone is a typical antipsychotic agent. Two simple, sensitive and accurate spectrophotometric methods have been developed for the determination of Ziprasidone hydrochloride (ZPD) in pure state and in its pharmaceutical formulations. The developed Method A is based on the oxidation of the drug with Fe(III) and subsequent chelation of Fe(II) produced with 2,21 Bipyridyl to produce colored chromogen having maximum absorption at λmax 510 nm and Linearity in the range of 40-200 μg/mL. Method B involves oxidation followed by complex formation of the drug with Bathophenanthroline and it exhibits maximum absorption at λmax 630 nm; Linearity in the range of 4-20 μg/mL. The results obtained were statistically evaluated and were found to be accurate and reproducible. Source


Sreelakshmi A.,Montessori Mahila Kalasala
Oriental Journal of Chemistry | Year: 2010

Mizolastine is an antihistamine drug. Two simple, sensitive and accurate spectrophotometric methods have been developed for the determination of Mizolastine in pure state and in its pharmaceutical formulations. The developed Method A is based on the formation of picrate salt between picric acid and free base of Mizolastine and it shows maximum absorption at λ max 400 nm and Linearity in the range of 3-15 μg/mL. Method B involves reaction between free base of Mizolastine and chloranilic acid. The developed chromogen In Method B shows maximum absorption at λ max 530 nm and Linearity in the range of 50-250 μg/mL. The results obtained were statistically evaluated and were found to be accurate and reproducible. Source


Chandrasekhara Reddy M.,Montessori Mahila Kalasala | Chandrasekhara Reddy M.,Krishna University | Bramhachari P.V.,Krishna University | Sri Rama Murthy K.,Montessori Mahila Kalasala
Tropical and Subtropical Agroecosystems | Year: 2015

In the present study effect of different concentrations of sucrose and Thidiazuron (TDZ) on in vitro morphogenesis of C. ensifolia was investigated and rapid micropropagation protocol was developed from in vitro derived nodal explants. Among the sucrose concentrations tested, medium concentration (3%) of sucrose induced maximum number of healthy shoots (6.5±0.68). The shoots formed in this concentration are normal and elongated rapidly. Whereas at higher concentrations delayed shoot induction with stout shoots stunted in their growth was observed. Among different concentrations of TDZ tested healthy shoots with well developed leaves were formed in lower concentration i.e. 0.2 mg/l. the number of shoots formed in this concentration was limited to 3.6±0.18 shoots/explant. The shoots raised in vitro were best rooted on MS medium supplemented with 0.1 mg/l α-Naphthaleneacetic acid (NAA) in combination with 0.05 mg/l 6-Benzyladenine (BAP). The maximum root induction (84%) with mean number of roots of 6.7±0.26 and with mean root length 3.5±0.28 cm was observed after six weeks of inoculation. Complete plantlets developed in vitro were acclimatized successfully with 85% survival in field conditions. Source


Murthy K.S.R.,Montessori Mahila Kalasala | Kondamudi R.,Montessori Mahila Kalasala
Phytomorphology: An International Journal of Plant Morphology | Year: 2010

Nodal and nodal TCLs explants of endangered Ceropegia spiralis and C. pusilla were cultured on Murashige & Skoog media (full, half and quarter strengths) supplied with sucrosc, cytokinins and auxins alone and in combinations. Flowering was achieved after 5-10 weeks of culture. In C. spiralis, the maximum flowering (84%) was observed on Vi MS medium supplemented with NAA 10.74 μM/l, 3% sucrose and in C. pusilla, the maximum flowering (80%) was recorded on Vi MS containing IB A 0.0123 μM/l, with 3% sucrose. Although these flowers are smaller in size, the in vitro tlowers were morphologically comparable with that of in vivo derived tlowers. Source

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