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Ulaanbaatar, Mongolia

Takeda S.,Minami Nippon Dairy Co op. | Takeda S.,University of Miyazaki | Takeshita M.,Minami Nippon Dairy Co op. | Kikuchi Y.,Minami Nippon Dairy Co op. | And 6 more authors.
International Immunopharmacology | Year: 2011

Some probiotics possess immunomodulatory activities and have been used as complementary and alternative medicines. We previously found that 10 lactic acid bacteria (LAB) strains isolated from traditional Mongolian dairy products showed probiotic potential in vitro. In this study, we assessed the immunomodulatory activity of 10 LABs on influenza virus (IFV) infection in relation to their efficacies in IFV-infected mice. In an intranasal IFV infection model in mice, oral administration of boiled Lactobacillus plantarum 06CC2 strain (20 mg/mouse), one of the 10 LABs, twice daily for 10 days starting two days before infection was significantly effective in protecting the body weight loss of infected mice, reducing virus yields in the lungs on days 2, 4, and 6 after infection, and prolonging survival times without toxicity. The total numbers of infiltrated cells in the bronchoalveolar lavage fluid (BALF), especially macrophages and neutrophils, were significantly reduced by 06CC2 administration on day 2. On day 2, tumor necrosis factor (TNF)-α production in BALF was also reduced significantly, but interferon-α, interleukin-12, and interferon-γ productions were augmented and natural killer (NK) cell activity was significantly elevated. Furthermore, the gene expressions of interleukin-12 receptor and interferon-γ in Peyer's patches were augmented by 06CC2 administration on day 2. Thus, 06CC2 was suggested to alleviate influenza symptoms in mice in correlation with the augmentation of NK cell activity associated with the enhancement of interferon-α and Th1 cytokine productions through intestinal immunity and the reduction of TNF-α in the early stage of infection. © 2011 Elsevier B.V. Source


Biswas G.,University of Miyazaki | Korenaga H.,University of Miyazaki | Nagamine R.,University of Miyazaki | Kawahara S.,University of Miyazaki | And 6 more authors.
International Immunopharmacology | Year: 2013

The important role played by cytokines in host innate immunity and the interaction of subsets of immune and inflammatory cells through cytokines offer avenues for immune interventions. We investigated 16 cytokine gene responses in the Japanese pufferfish, Takifugu rubripes orally treatedwith a heat-killed lactic acid bacterium (LAB), Lactobacillus paracasei spp. paracasei (strain 06TCa22) (Lpp) isolated from a Mongolian dairy product at 1 mg g-1 bodyweight d-1 for 3 days. Additionally,we assessed superoxide anion production (SAP) and phagocytic activity (PA) of head kidney cells and resistance to Vibrio harveyi infection in treated fish. Significant up-regulation of pro-inflammatory (IL-1β, IL-6, IL-17A/F-3, TNF-aα and TNF-N), cell-mediated immunity inducing (IL-12p35, IL-12p40 and IL-18), antiviral/intra-cellular pathogen killing (I-IFN-1 and IFN-γ), anti-inflammatory (IL-10) and peripheral T cell expansion and survival controlling (IL-2, IL-7, IL-15, IL-21 and TGF-β1) cytokines was observed in the treated fish. Furthermore, significantly increased SAP, PA and pathogen resistance were observed in the treated fish compared to untreated fish. Our results indicate the enhancement of cytokine mediated immunity in T. rubripes by the use of the heat-killed Lpp as a potential immunostimulant andwould be of great use in immunomodulation trialswith the possibility tomonitor positive immune response. © 2013 Elsevier B.V. All rights reserved. Source


Takeda S.,Minami Nihon Rakuno Kyodo Co. | Kawahara S.,University of Miyazaki | Hidaka M.,Kyushu University of Health and Welfare | Yoshida H.,Kyushu University of Health and Welfare | And 6 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2013

We investigated 10 lactic acid bacteria strains with probiotic potential prepared from Mongolian dairy products for their ability to induce T helper type-1 (Th1) cytokine production in mouse immune cells in vitro and in vivo. Among these strains, the Lactobacillus plantarum 06CC2 strain was effective in elevating the level of interleukin (IL)-12p40 in co-culture with J774.1 cells and the levels of IL-12 and interferon (IFN)-γ in co-culture with mouse spleen cells in vitro. Oral administration of this strain augmented the gene expression of IFN-γ and IL-12p40 and enlarged the population of CD4 +, CD25+, and CD49b+ cells in the spleens of normal mice. It also significantly elevated the gene expression of IL-12 receptor β2 as well as IL-12p40 and IFN-γ in Peyer's patches. Thus oral administration of strain 06CC2 was effective in inducing Th1 cytokine production activating the Th1 immune response associated with intestinal immunity in normal mice. Source


Takeda S.,Minami Nihon Rakuno Kyodo Co. | Fujimoto R.,Minami Nihon Rakuno Kyodo Co. | Takenoyama S.-I.,Minami Kyushu University | Takeshita M.,Minami Nihon Rakuno Kyodo Co. | And 5 more authors.
Food Science and Technology Research | Year: 2013

We screened suitable lactic acid bacteria (LAB) strains for use in fermented dairy products by analyzing milk fermented with 10 probiotic LAB strains from Mongolian dairy products. Lactobacillus paracasei paracasei strain 06TCa19 was selected because of its favorable effects on pH, lactic acid production, and viable bacterial numbers after fermentation of skim milk. Then, we prepared 06TCa19 and control fermented milks and conducted a randomized, double-blind crossover study with 46 healthy women to determine the effects of the strain on human defecation. The ingestion of 06TCa19 fermented milk improved the subjects' fecal characteristics, including shape and color. Analysis of stool samples from 8 subjects revealed that l-lactic acid levels and Lactobacillus and Bifidobacterium numbers increased. Moreover, strain 06TCa19 was suggested to reach and survive in the intestines, and is, therefore, suitable for fermented dairy products and can potentially improve human defecation. Source


Takeda S.,Minami Nihon Rakuno Kyodo Co. | Hidaka M.,Kyushu University of Health and Welfare | Yoshida H.,Kyushu University of Health and Welfare | Takeshita M.,Minami Nihon Rakuno Kyodo Co. | And 7 more authors.
Journal of Functional Foods | Year: 2014

Antiallergic activities of 10 lactic acid bacteria strains prepared from Mongolian dairy products as orally administered probiotics were examined in three murine type I allergy models (compound 48/80 stimulation, passive cutaneous anaphylaxis reaction, and ovalbumin sensitization models). Among the 10, only Lactobacillus plantarum strain 06CC2 significantly alleviated allergic symptoms in all three models and reduced the levels of total IgE, ovalbumin-specific IgE, and histamine in the sera of ovalbumin-sensitized mice. In vitro study, interferon-γ and interleukin-4 secretions from spleen cells of ovalbumin-sensitized mice administered the 06CC2 strain were significantly enhanced and suppressed, respectively, in the presence of ovalbumin. In Peyer's patches of ovalbumin-sensitized mice, strain 06CC2 significantly enhanced mRNA expressions of interferon-γ and interleukin-12 receptor β2, but suppressed that of the interleukin-4. Thus, strain 06CC2 probably promoted Th1 immunity through intestinal immunity and improved the Th1/Th2 balance in type I allergic mice, resulting in alleviation of allergic symptoms. © 2014 Elsevier Ltd. Source

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