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Bundoora, Australia

Zhou M-P.,Jiangsu Academy of Agricultural Sciences | Hayden M.J.,Molecular Plant Breeding CRC | Zhang Z.,Chinese Academy of Agricultural Sciences | Lu W.-Z.,Jiangsu Academy of Agricultural Sciences | Ma H.-X.,Jiangsu Academy of Agricultural Sciences
Journal of Applied Genetics | Year: 2010

Fusarium head blight (FHB) is a destructive disease in wheat. The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of the resistance to FHB worldwide, but the discrepancy in reported location of the major QTL could block its using in map based cloning and marker assisted selection. In this study, Chinese Spring-Sumai 3 chromosome 3B substitution line was used as resistant parent of the mapping population to reduce the confounded effect of genetic background in Sumai 3. The major QTL region was saturated with the Sequence Tagged Microsatellite (STM) and Sequence Tagged Site (STS) markers. A linkage map of chromosome 3B with 36 markers covering a genetic distance of 112.4 cM was constructed. Twelve new markers were inserted into the chromosome region where the major QTL was located. The average interval distance between markers was 1.5 cM. Multiple QTL Models (MQM) mapping indicated that the major QTL was located in the interval of Xgwm533 -Xsts9-1, and explained 45.6% of phenotypic variation of the resistance to FHB. The SSR (simple sequence repeat) marker Xgwm533 and STM marker Xstm748tcac are closely linked to the major QTL.

Panter S.,Molecular Plant Breeding CRC | Panter S.,Australian Department of Primary Industries and Fisheries | Chu P.G.,CSIRO | Ludlow E.,Molecular Plant Breeding CRC | And 17 more authors.
Transgenic Research | Year: 2012

Viral diseases, such as Alfalfa mosaic virus (AMV), cause significant reductions in the productivity and vegetative persistence of white clover plants in the field. Transgenic white clover plants ectopically expressing the viral coat protein gene encoded by the sub-genomic RNA4 of AMV were generated. Lines carrying a single copy of the transgene were analysed at the molecular, biochemical and phenotypic level under glasshouse and field conditions. Field resistance to AMV infection, as well as mitotic and meiotic stability of the transgene, were confirmed by phenotypic evaluation of the transgenic plants at two sites within Australia. The T 0 and T 1 generations of transgenic plants showed immunity to infection by AMV under glasshouse and field conditions, while the T 4 generation in an agronomically elite 'Grasslands Sustain' genetic background, showed a very high level of resistance to AMV in the field. An extensive biochemical study of the T 4 generation of transgenic plants, aiming to evaluate the level and composition of natural toxicants and key nutritional parameters, showed that the composition of the transgenic plants was within the range of variation seen in non-transgenic populations. © 2011 Springer Science+Business Media B.V.

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