Molecular Mutagenesis and DNA Repair Unit

Genova, Italy

Molecular Mutagenesis and DNA Repair Unit

Genova, Italy

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Ciribilli Y.,University of Trento | Monti P.,Molecular Mutagenesis and DNA Repair Unit | Bisio A.,University of Trento | Nguyen H.T.,University of California at San Diego | And 9 more authors.
Nucleic Acids Research | Year: 2013

Structural and biochemical studies have demonstrated that p73, p63 and p53 recognize DNA with identical amino acids and similar binding affinity. Here, measuring transactivation activity for a large number of response elements (REs) in yeast and human cell lines, we show that p53 family proteins also have overlapping transactivation profiles. We identified mutations at conserved amino acids of loops L1 and L3 in the DNA-binding domain that tune the transactivation potential nearly equally in p73, p63 and p53. For example, the mutant S139F in p73 has higher transactivation potential towards selected REs, enhanced DNA-binding cooperativity in vitro and a flexible loop L1 as seen in the crystal structure of the protein-DNA complex. By studying, how variations in the RE sequence affect transactivation specificity, we discovered a RE-transactivation code that predicts enhanced transactivation; this correlation is stronger for promoters of genes associated with apoptosis. © 2013 The Author(s). Published by Oxford University Press.

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