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Fei L.-N.,Huaqiao University | Fei L.-N.,Molecular Medicine Engineering Research Center | Wang Q.-Z.,Huaqiao University | Wang Q.-Z.,Molecular Medicine Engineering Research Center | And 2 more authors.
Yaoxue Xuebao | Year: 2012

RNA interference (RNAi) has been proved as a novel approach for gene therapy. However, RNAi mono-therapy only aims at single gene, it therefore may ultimately fail to cure cancers caused by polygene variation. To overcome the deficiency of RNAi mono-therapy, "combinatorial RNA interference" (coRNAi) was put forward as a new strategy. By co-expressing the inducers of RNAi triggering single or multiple targets directly and other RNA- or protein-based silencers, coRNAi keeps target genes silent, prevents carcinogenic progression and induces apoptosis of tumor cells. This paper mainly reviews the major strategies of coRNAi and their applications in cancer gene therapy.

Wang Q.,Huaqiao University | Wang Q.,Molecular Medicine Engineering Research Center | Lu Y.,Huaqiao University | Lu Y.,Molecular Medicine Engineering Research Center | And 6 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2011

Recombinant adeno-associated viral vectors (rAAV) have been widely used as gene therapy vectors in clinical trials. Here, we reviewed the genomic structures and replication mechanisms of wt-AAV. Then, the assembly of capsid and the encapsidation of genomic DNA, two major events during AAV pakaging, was discussed in detail. Although the overall pattern of virus assembly and encapsidation is known, the molecular mechanisms and the structure-function relationship involved in these processes are not well understood. Further elucidatation of these processes may improve the production technology of rAAV and develop gene drug based on rAAV. © 2011 CJB, All rights reserved.

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