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Azzalin A.,University of Pavia | Moretti E.,University of Pavia | Arbustini E.,Molecular Genetic Laboratory Transplant Research Area Fondazione Irccs Policlinico S Matteo | Magrassi L.,University of Pavia | Magrassi L.,CNR Institute of Molecular Genetics
Journal of Neuro-Oncology | Year: 2014

Shc3 protein levels are high in human glioblastoma but they decrease in vitro. We found that SHC3 mRNA and protein increased when glioblastoma cells grew as multicellular tumor spheroid (MTS). Shc3 expression was also induced in adherent cultures by increasing cell density. Among the Shc family members, only Shc2 and Shc3 increased with cell density. Shc3 and focal adhesion kinase (Fak) interact as shown by co-immunoprecipitation. Inhibition of Fak activation reduced Shc3 increase and MTS formation and changed Shc3 phosphorylation pattern. Our results suggest that in gliomas cell density modulates Shc3 protein levels and its activity, at least in part, through Fak activation. © Springer Science+Business Media New York 2014.


Magrassi L.,University of Pavia | Magrassi L.,CNR Institute of Molecular Genetics | Marziliano N.,Molecular Genetic Laboratory Transplant Research Area Fondazione Irccs Policlinico S Matteo | Inzani F.,Molecular Genetic Laboratory Transplant Research Area Fondazione Irccs Policlinico S Matteo | And 7 more authors.
Cancer Letters | Year: 2010

By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo. © 2009 Elsevier Ireland Ltd. All rights reserved.

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