Molecular Devices | Date: 2015-01-20
A plenum assembly configured for electrophysiology assays, such as patch clamp techniques, includes one or more ground electrode assemblies. The ground electrode assemblies are individually removable from a plenum base of the plenum assembly in a non-destructive manner, and may be reinstalled in the plenum base in a manner that reestablishes electrical contact with ground circuitry without requiring soldering or other additional steps. A rejuvenating apparatus is provided for rejuvenating one or more ground electrode assemblies removed from the plenum base.
Molecular Devices | Date: 2015-07-30
An optical element holder includes receptacles for retaining optical elements, and is configured to move a selected optical element into an optical path whereby a light beam passes through the selected optical element. The optical element holder is configured to retain the optical elements in a manner that mitigates or avoids misalignment of the optical elements, thereby mitigating or avoiding unwanted deviations in the path of the light beam. The optical element holder may be part of a microscope or other optical instrument.
Molecular Devices | Date: 2015-08-14
System, including methods and apparatus, for sample processing. In exemplary embodiments, the system comprises a plurality of devices to perform a protocol on sample holders supporting samples, and also comprises a control system that coordinates operation of the plurality of devices, such that the protocol is performed automatically. Each device of at least two of the plurality of devices may have one or more status lights configured to display a plurality of different visual indicators each indicating a different status of the device. The at least two devices may utilize a same indicator scheme as one another for each different status indicated by the visual indicators. In some embodiments, the indicator scheme is user-configurable. In some embodiments, the one or more status lights of at least one device are provided by one or more recessed light-emitting strips.
Molecular Devices | Date: 2015-10-19
Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.
Molecular Devices | Date: 2015-01-23
System, including methods and apparatus, for identifying and picking spectrally distinct colonies. In an exemplary method, a filter may be received in an optical path extending from a light source to a grayscale image detector. The filter may be configured to increase an intensity difference between a first type of colony and a spectrally distinct second type of colony. Colonies including both types may be received in the optical path. An image of the colonies may be obtained with the grayscale image detector. At least one of the types of colony may be identified in the image. One or more colonies of the at least one type may be picked robotically.
Molecular Devices | Date: 2016-01-21
A system and method to segment an image captured from an image capture device of a high content imaging system includes an image acquisition module that receives the image captured by the image capture device. A coarse object detection module develops a coarse segmented image, wherein each pixel of the coarse segmented image is associated with a corresponding pixel in the captured image and is identified as one of an object pixel and a background pixel. A marker identification module selects at least one marker pixel from the pixels of the coarse segmented image, wherein each marker pixel is one of a contiguous group of object pixels in the coarse segmented image that is furthest from a background pixel relative to neighboring pixels of the group. An object splitting module that comprises a plurality of processors operating in parallel that associates each object pixel of the coarse segmented image with a marker pixel, wherein a distance based metric between the object pixel and the marker pixel is less than the distance based metric between the object pixel and any other marker pixel in the coarse segmented image.
Molecular Devices | Date: 2016-02-09
A system and method for analyzing multi-dimensional images includes a high content imaging system that includes an image capture device. An image acquisition module receives a series of images of a biological sample captured by the image capture device, and the series of images includes a sequence of image planes. A human interface module receives from a user computer specifications of a first image analysis step and a second image analysis step. The first image analysis step specifies a first image processing operation that processes an image plane of a series of images in accordance with at least another image plane of the series of images and the second image analysis step specifies a second image processing operation that processes each image plane of a series of images independently of the other image planes of the series. An image analysis module having a plurality of processors operating in parallel processes the first series of images in accordance with the first image processing step to generate a first output series of images, and processes the first series of images in accordance with second image processing step to generate a second output series of images. The human interface module displays at least one image plane of the first output series of images and at least one image plane of the second output series of images on a display associated with the user computer.
Molecular Devices | Date: 2015-07-22
A system is provided for performing filter-based and monochromator-based measurements. The system includes a light source (250) and a plurality of detectors (112, 114). An excitation monochromator (130) outputs a selected wavelength component of the excitation light. Emitted light from a sample follows a selected emission optical path. An emission monochromator (132) outputs a selected wavelength component of the emitted light when part of the selected path. An interface cartridge (104) includes emission light ports positioned to direct the emitted light from the sample along a corresponding optical path. The interface cartridge aligns a selected optical path with the main measurement optical axis. A movable sliding switch mechanism (120) provides optical channels corresponding to positions on the sliding switch mechanism to complete a selected emission optical path. The position on the sliding switch mechanism is selected by moving the sliding switch mechanism to align the optical channel for the position with the main measurement optical axis.
Molecular Devices | Date: 2016-02-25
The present invention is directed to multiplexed fluorescence detection, including time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission and spectral differences in excitation is used to enhance the ability to separate signals in an assay from multiple fluorescent labels. Different classes of labels may be utilized, including upconversion phosphors as well as lanthanide chelates and transition metal chelates. The methods may be implemented in optical plate readers, including cartridge-based multi-mode readers.
Molecular Devices | Date: 2016-02-10
The invention relates to a computer-implemented method for counting micronuclei of a plurality of cells in a cell-containing sample, the method comprising:receiving (902) a raw image of the cell-containing sample;receiving (904) one or more micronuclei parameters specifying an estimated size, a minimum intensity, and a distance range from a main nucleus for micronuclei in the raw image;transforming (908) the raw image into a second segmented image comprising one or more unattached micronuclei based on the one or more micronuclei parameters;transforming (910) the raw image into a third segmented image comprising one or more nuclei clusters based on the one or more micronuclei parameters such that the third segmented image has sufficient resolution to include attached micronuclei in the one or more nuclei clusters;detecting (912) any attached micronuclei in the one or more nuclei clusters in the third segmented image;assigning (914) each of the unattached and the attached micronuclei to a cell among the plurality of cells; andcalculating (916) a micronuclei count for each of the plurality of cells by tallying the unattached and the attached micronuclei that are assigned to the cell.