Molecular Devices | Date: 2015-01-20
A plenum assembly configured for electrophysiology assays, such as patch clamp techniques, includes one or more ground electrode assemblies. The ground electrode assemblies are individually removable from a plenum base of the plenum assembly in a non-destructive manner, and may be reinstalled in the plenum base in a manner that reestablishes electrical contact with ground circuitry without requiring soldering or other additional steps. A rejuvenating apparatus is provided for rejuvenating one or more ground electrode assemblies removed from the plenum base.
Molecular Devices | Date: 2015-07-30
An optical element holder includes receptacles for retaining optical elements, and is configured to move a selected optical element into an optical path whereby a light beam passes through the selected optical element. The optical element holder is configured to retain the optical elements in a manner that mitigates or avoids misalignment of the optical elements, thereby mitigating or avoiding unwanted deviations in the path of the light beam. The optical element holder may be part of a microscope or other optical instrument.
Molecular Devices | Date: 2015-08-14
System, including methods and apparatus, for sample processing. In exemplary embodiments, the system comprises a plurality of devices to perform a protocol on sample holders supporting samples, and also comprises a control system that coordinates operation of the plurality of devices, such that the protocol is performed automatically. Each device of at least two of the plurality of devices may have one or more status lights configured to display a plurality of different visual indicators each indicating a different status of the device. The at least two devices may utilize a same indicator scheme as one another for each different status indicated by the visual indicators. In some embodiments, the indicator scheme is user-configurable. In some embodiments, the one or more status lights of at least one device are provided by one or more recessed light-emitting strips.
Molecular Devices | Date: 2015-10-19
Microscope system for, and methods of, imaging a sample including biological cells. In an exemplary method, light transmitted through the sample may be detected for a first set of focal positions to collect a first stack of images. Values of a focus metric may be calculated for the first stack of images. A candidate focal position may be determined based on the values. Photoluminescence may be detected from the sample for a second set of focal positions to collect a second stack of images. The second set of focal positions may define a smaller range than the first set of focal positions. At least one focal position of the second set of focal positions may be based on the candidate focal position. In other words, the candidate focal position may serve as a guide for finding a suitable photoluminescence focal position.
Molecular Devices | Date: 2015-07-22
A system is provided for performing filter-based and monochromator-based measurements. The system includes a light source (250) and a plurality of detectors (112, 114). An excitation monochromator (130) outputs a selected wavelength component of the excitation light. Emitted light from a sample follows a selected emission optical path. An emission monochromator (132) outputs a selected wavelength component of the emitted light when part of the selected path. An interface cartridge (104) includes emission light ports positioned to direct the emitted light from the sample along a corresponding optical path. The interface cartridge aligns a selected optical path with the main measurement optical axis. A movable sliding switch mechanism (120) provides optical channels corresponding to positions on the sliding switch mechanism to complete a selected emission optical path. The position on the sliding switch mechanism is selected by moving the sliding switch mechanism to align the optical channel for the position with the main measurement optical axis.
Molecular Devices | Date: 2015-07-24
In a method for measuring luminescence of a biological sample utilizing two different luminescence reagents, the sample is agitated to improve mixing with the second luminescence reagent, allowing for a shorter delay time between injection of the second reagent and measurement of the resulting luminescence activity. The improved mixing may also allow for a shorter measurement time, thereby improving throughput when assaying a large number of samples.
Molecular Devices | Date: 2016-02-25
The present invention is directed to multiplexed fluorescence detection, including time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission and spectral differences in excitation is used to enhance the ability to separate signals in an assay from multiple fluorescent labels. Different classes of labels may be utilized, including upconversion phosphors as well as lanthanide chelates and transition metal chelates. The methods may be implemented in optical plate readers, including cartridge-based multi-mode readers.
Molecular Devices | Date: 2015-10-20
An apparatus for automatically conditioning a patch plate and a plenum of an electrophysiology measurement system is provided. An arm is linearly movable between a non-operative position and an operative position. An end effector mounted to one side of the arm is configured to condition the patch plate. Another end effector mounted to an opposite side of the arm is configured to condition the plenum. A linear actuator is coupled to the arm and is configured to drive movement of the arm between the operative position and the non-operative position. When the arm is positioned in the operative position, the arm is situated between the patch plate and the plenum.
Molecular Devices | Date: 2015-07-29
The invention relates to a system and a method for measuring a target in a sample (512), the target being capable of generating an emitted light (505) and/or transmitted light (707) in response to an excitation light (503, 703). In an example system, an excitation light source (504) generates the excitation light along an excitation optical path. An attenuation filter arrangement (506) selectively adds an attenuation filter to the excitation optical path. The attenuation filter attenuates the excitation light by a corresponding attenuation factor. The excitation light exits the attenuation filter arrangement (506) along the excitation optical path to illuminate the sample. A light energy detector (516) receives the emitted light generated in response to the excitation light, and outputs a measured signal level corresponding to an emitted light level. If the light energy detector (516) indicates an overflow, signal measurement is repeated with attenuation filters of increasing attenuation factors until the measured signal level does not overflow.
Molecular Devices | Date: 2016-02-10
The invention relates to a computer-implemented method for counting micronuclei of a plurality of cells in a cell-containing sample, the method comprising:receiving (902) a raw image of the cell-containing sample;receiving (904) one or more micronuclei parameters specifying an estimated size, a minimum intensity, and a distance range from a main nucleus for micronuclei in the raw image;transforming (908) the raw image into a second segmented image comprising one or more unattached micronuclei based on the one or more micronuclei parameters;transforming (910) the raw image into a third segmented image comprising one or more nuclei clusters based on the one or more micronuclei parameters such that the third segmented image has sufficient resolution to include attached micronuclei in the one or more nuclei clusters;detecting (912) any attached micronuclei in the one or more nuclei clusters in the third segmented image;assigning (914) each of the unattached and the attached micronuclei to a cell among the plurality of cells; andcalculating (916) a micronuclei count for each of the plurality of cells by tallying the unattached and the attached micronuclei that are assigned to the cell.