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Alshatwi A.A.,Molecular Cancer Biology Research Laboratory | Shafi G.,Molecular Cancer Biology Research Laboratory | Hasan T.,Molecular Cancer Biology Research Laboratory | Lsaif M.,King Saud University | Lei D.K.,University of Maryland College Park
Asian Pacific Journal of Cancer Prevention | Year: 2011

Certain single nucleotide polymorphisms (SNPs) in genes like p21 or bcl2 increase susceptibility to breast cancer but it has not, until now, been clear whether common polymorphic variants in the same genes also increase risk in Saudi Arabian population. The aim of this study was therefore to determine whether polymorphisms of p21 or Bcl2 might be associated with an increased risk of breast cancer in Saudi women. p21 (rs733590) C/T SNP was not found to be associated with breast cancer pathogenesis. However, we found that a reverse mutation T/C might be linked with breast cancer occurrence. Bcl2 genotypes were marginally associated overall with breast cancer risk. In addition, the alleles of this gene were significantly associated with risk of breast cancer. The allelic frequency of G was higher (0.68) in patients than in healthy women. AA vs. AG+GG genotype [OR=3.56 (1.24-10.68); P=0.008] was the dominant genotype. It is likely that these genes conferring measurably increased risks of breast cancer in our study population. Source


Alshatwi A.A.,Molecular Cancer Biology Research Laboratory | Shafi G.,Molecular Cancer Biology Research Laboratory | Hasan T.N.,Molecular Cancer Biology Research Laboratory | Alsaif M.A.,King Saud University | And 3 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2011

Dietary phytochemicals have a variety of antitumor properties. In the present study, the antitumor activity of methanolic extract of lemon fruit (lemon extract; LE) (LE) on the MCF-7 breast cancer cell line was investigated in vitro. Apoptotic cell death was analyzed using the TUNEL assay. In addition, the apoptosis mediated by LE extract in the MCF-7 cells was associated with the increased expression of the tumor suppressor p53 and caspase-3. Additionally, the expression of a pro-apoptotic gene, bax, was increased, and the expression of an anti-apoptotic gene, bcl-2, was decreased by LE extract treatment, resulting in a shift in the Bax:Bcl-2 ratio to one that favored apoptosis. The expression of a major apoptotic gene, caspase-3, was increased by LE extract treatment. In light of the above results, we concluded that LE extract can induce the apoptosis of MCF-7 breast cancer cells via Bax-related caspase-3 activation. This study provides experimental data that are relevant to the possible future clinical use of LE to treat breast cancer. Source

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