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Gao H.,Biochemistry and Molecular Biology Teaching and Research Section | Zhou T.,Biochemistry and Molecular Biology Teaching and Research Section | Li S.,Biochemistry and Molecular Biology Teaching and Research Section | Zhang C.,Biochemistry and Molecular Biology Teaching and Research Section | Chen H.,Biochemistry and Molecular Biology Teaching and Research Section
Engineering Technology, Engineering Education and Engineering Management - International Conference on Engineering Technology, Engineering Education and Engineering Management, ETEEEM 2014 | Year: 2015

It aims to analyze the impact heme oxygenase -1 (heme oxygenase 1, HO-1) on regulating factors of human hepatoma cell HepG2’s cell cycle, through constructing recombinant vector of pcDNA3.1 containing wild-type and mutant HO-1 gene (+)-wtHO-1 and pcDNA3.1 (+)-mHO-1G143H. By using the method of liposome-mediated, the recombinant vector was transfected hepatoma cell line HepG2. And the transfected one with empty vector was treated as a control group. By the selection of G418, stable expression of wild-type and mutant HO-1 in HepG2 liver cancer cell lines were established. Use the blot of semi- quantitative RT-PCR and Western to test transfected cell lines expressing levels of HO-1 mRNA and protein. As HO-1 expression in stably transfected cell lines altered, we use Western blot to test transfected cell lines P21, P27 protein expression levels. As result shows, we got 1 HO-over-expression of wild-type and mutant in HepG2 cells; wild-type and mutant’s over expression of HO-1 can induce the expression of tumor suppressor genes p21 and p27.we got the conclusion that HO-1’s over-expression of tumor suppressor genes p21 and p27 is unrelated to the expression of heme decomposition products. HO-1 may regulate the expression of p21 and p27 through other mechanisms. © 2015 Taylor & Francis Group, London.

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