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Monteso-Curto P.,Rovira i Virgili University | Ferre-Grau C.,Rovira i Virgili University | Lleixa-Fortuno M.,Rovira i Virgili University | Albacar-Rioboo N.,Rovira i Virgili University | Lejeune M.,Molecular Biology and Research Section
Archives of Psychiatric Nursing | Year: 2014

The aims of this study were to explore the prevalence and the conceptualizations of depression detected by the healthcare system, identified by the patient or classified/identified in the validated Goldberg's questionnaire in a community. We conducted a cross-sectional evaluation of 317 patients. The different types of depression diagnosed, identified, current or total were stratified by age and gender groups. The difference in the conceptualization of depression from the medical or ordinary people point of view indicate that depression care requires the understanding of the lifestyle, beliefs, attitudes, family and social networks of the people the physicians and nurses care for. © 2014 Elsevier Inc.

Lopez C.,Unitat de Suport A la Recerca de la Gerencia Territorial Terres de LEbre | Tomas B.,Hospital de Tortosa Verge de la Cinta | Korzynska A.,Nalecz Institute of Biocybernetics and Biomedical Engineering | Bosch R.,Hospital de Tortosa Verge de la Cinta | And 6 more authors.
American Journal of Clinical Pathology | Year: 2013

A new method that simplifies the evaluation of the traditional HER2 fluorescence in situ hybridization (FISH) evaluation in breast cancer was proposed. HER2 status was evaluated in digital images (DIs) captured from 423 invasive breast cancer stained sections. All centromeric/CEP17 and HER2 gene signals obtained from separated stacked DIs were manually counted on the screen. The global ratios were compared with the traditional FISH evaluation and the immunohistochemical status. The 2 FISH scores were convergent in 96.93% of cases, showing an "almost perfect" agreement with a weighted k of 0.956 (95% confidence interval, 0.928-0.985). The new method evaluates at least 3 times more nuclei than traditional methods and also has an almost perfect agreement with the immunohistochemical scores. The proposed enhanced method substantially improves HER2 FISH assessment in breast cancer biopsy specimens because the evaluation of HER2/CEP17 copy numbers is more representative, easier, and faster than the conventional method. © American Society for Clinical Pathology.

Lejeune M.,University of Barcelona | Lejeune M.,Molecular Biology and Research Section | Miro J.M.,University of Barcelona | Lazzari E.D.,University of Barcelona | And 17 more authors.
Clinical Infectious Diseases | Year: 2011

Background: It is unknown whether a Toxoplasma gondii-specific T cell response is restored after successful combined antiretroviral therapy (cART) in patients with AIDS and current or previous toxoplasmic encephalitis (TE). Methods: We performed a multicenter cross-sectional study with 17 healthy T. gondii-positive human immunodeficiency virus (HIV)-1-uninfected individuals and 90 patients coinfected with HIV-1 and T. gondii distributed in 5 groups according to their CD4+ T cell counts and T. gondii infection (with or without current or previous TE). We investigated the lymphocyte proliferative response (LPR) and interferon (IFN)-γ production in response to T. gondii soluble antigen extract (SATg) and as CD4+ and CD8+ T cell subsets. Results: SATg-specific LPR and IFN-γ production were not observed in many of the most immunosuppressed patients (CD4+ T cell count, <200 cells/μL, with or without current or previous TE). By contrast, these responses occurred in a considerable percentage (LPR, 43%; IFN-γ production, 80%) of patients receiving successful cART (CD4 + T cell count, >200 cells/μL) who presented with TE and had already stopped secondary TE prophylaxis. Similar results were found in immunocompetent asymptomatic patients who did not receive TE prophylaxis. The predictors of SATgspecific T cell responses and IFN-γ production were a cART-mediated increase in CD4+ T cell count and LPR to phytohemagglutinin and viral suppression and a decrease in the activated (CD38+) CD8+ T cell count, respectively. Conclusions: cART restores T. gondii-specific CD4 T cell responses in most patients with AIDS who had previous TE. Our data support the safety of withdrawing TE prophylaxis when the CD4+ T cell count returns to levels >200 cells/μL. © The Author 2011.

Roszkowiak L.,Nalecz Institute of Biocybernetics and Biomedical Engineering | Korzynska A.,Nalecz Institute of Biocybernetics and Biomedical Engineering | Lejeune M.,Molecular Biology and Research Section | Bosch R.,Hospital de Tortosa Verge de la Cinta | Lopez C.,Molecular Biology and Research Section
Advances in Intelligent Systems and Computing | Year: 2016

We present the METINUS (METhod of Immunohistochemical NUclei Segmentation), which is a improved and modified version of supporting tool for pathologists from 2010. The method supports examination of immunohistochemically stained thin tissue sections from biopsy of follicular lymphoma patients. The software localizes and counts FOXP3 expression in the cells’ nuclei supporting standard procedure of diagnosis and prognosis. The algorithm performs colour separation followed by object extraction and validation. Objects with statistical parameters not in specified range are disqualified from further assessment. To calculate the statistics we use the following: three channels of RGB, three channels of Lab colour space, brown channel and three layers completed with colour deconvolution. Division of the objects is done with support of watershed and colour deconvolution algorithm. Evaluation was performed on arbitrarily chosen 20 images with moderate quality of most typical tissues. We compared results of improved method with the previous version in the context of semiautomatic, pathologist controlled, computer-aided result of quantification as reference. Comparison is based on quantity of nuclei located per image using Kendall’s tau-b correlation coefficient. It shows concordance of 0.91 between results of proposed method and reference, while with previous version it is only 0.71. © Springer International Publishing Switzerland 2016.

De La Cruz-Merino L.,Hospital Universitario Virgen Macarena | Lejeune M.,Molecular Biology and Research Section | Nogales Fernandez E.,Hospital Universitario Virgen Macarena | Henao Carrasco F.,Hospital Universitario Virgen Macarena | And 5 more authors.
Clinical and Developmental Immunology | Year: 2012

Hodgkin's lymphoma represents one of the most frequent lymphoproliferative syndromes, especially in young population. Although HL is considered one of the most curable tumors, a sizeable fraction of patients recur after successful upfront treatment or, less commonly, are primarily resistant. This work tries to summarize the data on clinical, histological, pathological, and biological factors in HL, with special emphasis on the improvement of prognosis and their impact on therapeutical strategies. The recent advances in our understanding of HL biology and immunology show that infiltrated immune cells and cytokines in the tumoral microenvironment may play different functions that seem tightly related with clinical outcomes. Strategies aimed at interfering with the crosstalk between tumoral Reed-Sternberg cells and their cellular partners have been taken into account in the development of new immunotherapies that target different cell components of HL microenvironment. This new knowledge will probably translate into a change in the antineoplastic treatments in HL in the next future and hopefully will increase the curability rates of this disease. © 2012 Luis de la Cruz-Merino et al.

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