Hakimuddin F.,Molecular Biology and Genetics Laboratory |
Abidi F.,Molecular Biology and Genetics Laboratory |
Jafer O.,Molecular Biology and Genetics Laboratory |
Li C.,Molecular Biology and Genetics Laboratory |
And 3 more authors.
Biomolecular Detection and Quantification | Year: 2016
Beak and feather disease is caused by Circovirus, which affects actively growing beak and feather cells of avian species. The disease affects mainly young birds while older birds may overcome the disease with few lasting effects. Due to lack of treatment, the only way to control the disease is through hygiene and early diagnosis. As a diagnostic tool, we have established a Taqman probe based real-time PCR assay to detect the presence of the viral genome in psittacine birds in UAE and reported the incidence of circovirus in different species of psittacine birds. The sensitivity of our assay was found to be very high with detection limit of up to 3.5. fg of DNA in the sample. The mean prevalence of circovirus was found to be 58.33% in African Grey Parrots, 34.42% in Cockatoos, 31.8% in amazon parrots and 25.53% in Macaws.The Taqman assay is a quick, reliable and sensitive detection method that has been instrumental in identifying this disease that was not previously reported in the region. © 2015 The Authors. Source