Luo K.,Modern Virology Research Center |
Li S.,Chinese Academy of Sciences |
Xie M.,Wuhan University |
Wu D.,Modern Virology Research Center |
And 7 more authors.
Biochemical and Biophysical Research Communications
Prion diseases are fatal neurodegenerative disorders resulting from structural conversion of the cellular isoform of PrPC to the infectious scrapie isoform PrPSc. It is believed that such structural alteration may occur within the internalization pathway. However, there is no direct evidence to support this hypothesis. Employing quantum dots (QDs) as a probe, we have recorded a real-time movie demonstrating the process of prion internalization in a living cell for the first time. The entire internalization process can be divided into four discrete but connected stages. In addition, using methyl-beta-cyclodextrin to disrupt cell membrane cholesterol, we show that lipid rafts play an important role in locating cellular PrPC to the cell membrane and in initiating PrPC endocytosis. © 2010 Elsevier Inc. All rights reserved. Source