Modern Institute of Technology MIT

Rishīkesh, India

Modern Institute of Technology MIT

Rishīkesh, India
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Lily M.K.,Modern Institute of Technology MIT | Bahuguna A.,Modern Institute of Technology MIT | Dangwal K.,Modern Institute of Technology MIT | Garg V.,Banasthali University
Annals of Microbiology | Year: 2010

Benzo [a] pyrene (BaP), a pentacyclic polyaromatic hydrocarbon, is 1 of the 12 target compounds defined in the new US Environmental Protection Agency strategy for controlling persistent, bioaccumulative, and toxic pollutants. We previously isolated a novel strain Bacillus subtilis BMT4i capable of utilizing BaP as sole source of carbon and energy. The present study investigated (1) whether the BaP degradation pathway is inducible, and (2) whether it is plasmid-encoded. Furthermore, physical (temperature, pH, and UV-induced photolysis of BaP) and chemical (BaP concentration, surfactant, and ionic strength) parameters for BaP degradation were determined. Our findings revealed a ten-fold enhanced degradation rate in induced vs non-induced culture in the presence of chloramphenicol, suggesting that the BaP degradation pathway is inducible. Physical methods demonstrated the lack of plasmid in BMT4i-a result further complimented by plasmid curing, which had no effect on BaP degradability thus a chromosomal localization can be inferred. Maximum BaP degradation in BMT4i was observed under the following physical and chemical conditions: 30°C, pH 8.0, UV-induced photolysis of BaP-basal salt mineral medium (BSM), 150 μg/ml BaP, 0.01% Tween-20, and 400-1,800 μM MgSO4. These conditions could be beneficial in the development and standardization of effective bioremediation protocols using B. subtilis BMT4i. © 2010 Springer-Verlag and the University of Milan.


Bahuguna A.,Modern Institute of Technology MIT | Lily M.K.,Modern Institute of Technology MIT | Munjal A.,Banasthali University | Singh R.N.,Sbs Pg Institute Of Biomedical Science And Research | Dangwal K.,Modern Institute of Technology MIT
Journal of Environmental Sciences | Year: 2011

A new bacterial strain DMT-7 capable of selectively desulfurizing dibenzothiophene (DBT) was isolated from diesel contaminated soil. The DMT-7 was characterized and identified as Lysinibacillus sphaericus DMT-7 (NCBI GenBank Accession No. GQ496620) using 16S rDNA gene sequence analysis. The desulfurized product of DBT, 2-hydroxybiphenyl (2HBP), was identified and confirmed by high performance liquid chromatography analysis and gas chromatography-mass spectroscopy analysis respectively. The desulfurization kinetics revealed that DMT-7 started desulfurization of DBT into 2HBP after the lag phase of 24 hr, exponentially increasing the accumulation of 2HBP up to 15 days leading to approximately 60% desulfurization of the DBT. However, further growth resulted into DBT degradation. The induced culture of DMT-7 showed shorter lag phase of 6 hr and early onset of stationary phase within 10 days for desulfurization as compared to that of non-induced culture clearly indicating the inducibility of the desulfurization pathway of DMT-7. In addition, Lysinibacillus sphaericus DMT-7 also possess the ability to utilize broad range of substrates as sole source of sulfur such as benzothiophene, 3,4-benzo DBT, 4,6-dimethyl DBT, and 4,6-dibutyl DBT. Therefore, Lysinibacillus sphaericus DMT-7 could serve as model system for efficient biodesulfurization of diesel and petrol. © 2011 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.


Saini R.,Patanjali Ayurved Ltd | Garg V.,Banasthali University | Dangwal K.,Modern Institute of Technology MIT
International Journal of Pharma and Bio Sciences | Year: 2012

Ficus palmata, Pyrus pashia and Ficus auriculata are the popular wild edible fruits of Uttarakhand. The present study aimed to evaluate their total phenolic, flavonoid contents, antioxidant and antiproliferative activities by using two so vents namely 80% aqueous methanol and acetone. The results showed highest phenolic and flavonoid contents in the Ficus pa mata acetone extract, while lowest in Ficus auriculata and Pyrus pashia methanol extracts respectively. Antioxidant activities were highest in the acetone extract of Ficus palmata and Pyrus pashia respectively. RP-HPLC analysis revealed abunda ce of gallic acid and catechin in all the fruit extracts, while traces of caffeic acid, coumaric acid and ellagic acid.Allthe fruit extracts possessed antiproliferative activities (22-63%) against cervical cancer cells C33A being highest in Ficus palmata, while showing no cytotoxicity to the normal peripheral blood mononuclear cells. Therefore, Ficus palmata fruitextracts could be used for the developing herbal formulation that can reduce the oxidative stress and prevent cancer development.


Saini R.,Patanjali Ayurved Ltd. | Garg V.,Banasthali University | Dangwal K.,Modern Institute of Technology MIT
Food Science and Biotechnology | Year: 2013

Himalyan bayberry (Myrica esculenta) fruit in Indian Himalayan region was evaluated for total polyphenol contents, antioxidant, and anticancer activities in 4 different solvent systems namely 80% each of methanol, acidicmethanol, acetone, and acidic-acetone to ensure the optimum recovery. Himalyan bayberry acidic-acetone (MeAA) extracts showed highest recovery of phenolics, flavonoids, freeradical scavenging, and ferric-reducing activities followed by acetone (MeA), acidic-methanol (MeAM), and methanol (MeM) extracts. Both MeA and MeAA extracts showed potent anticancer activities leading to 70-92% reduction in the viability of C33A, SiHa, and HeLa cells while exhibiting no cytotoxicity towards normal transformed cell lines. The RP-HPLC analysis revealed abundance of gallic acid [793.74 mg/100 g of fruit weight (f.w.) in MeA], myricetin (345.6 mg/100 g f.w. in MeAA), caffeic acid (246.6 mg/100 g f.w. in MeM), catechin (190.181 mg/100 g f.w. in MeAA) while traces of chlorogenic acid (17.67 mg/100 g f.w. in MeAA), transcinnamic acid (5.91 mg/100 g f.w. in MeA), p-coumaric acid (5.84 mg/100 g f.w. in MeA), and ellagic acid (2.21 mg/100 g f.w. in MeA) corroborating the high antioxidant and anticancer activities of Himalyan bayberry fruit extracts. © 2013 The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.


Saini R.,Patanjali Ayurved Ltd | Dangwal K.,Modern Institute of Technology MIT | Singh H.,Modern Institute of Technology MIT | Garg V.,Banasthali University
Journal of Food Science and Technology | Year: 2012

Yellow Himalayan raspberry, a wild edible fruit, was analyzed for phenolic contents, and antioxidant, antibacterial and antiproliferative activities. Phenolics were extracted using 80 % aqueous solvents containing methanol, acidic methanol, acetone and acidic acetone. Our analysis revealed that the acidic acetone extracts recovered the highest level of total phenolics (899 mg GAE/100 g FW) and flavonoids (433.5 mg CE/100 g FW). Free radical scavenging activities (DPPH, ABTS, superoxide and linoleate hydroperoxide radicals) and ferric reducing activity were highest in the acetone and acidic acetone extracts. No metal chelating or antibacterial activity was detected in any of the extracts. Acetone and methanol extracts showed potent antiproliferative activity against human cervical cancer cells (C33A) with an EC50 of inhibition at 5.04 and 4. 9 mg/ml fruit concentration respectively, while showing no cytotoxicity to normal PBMCs cells. Therefore, the present study concluded that the yellow Himalayan raspberry is a potent source of phytochemicals having super antioxidant and potent antiproliferative activities. © 2012, Association of Food Scientists & Technologists (India).


PubMed | Banasthali University, Patanjali Ayurved Ltd and Modern Institute of Technology MIT
Type: Journal Article | Journal: Journal of food science and technology | Year: 2015

Yellow Himalayan raspberry, a wild edible fruit, was analyzed for phenolic contents, and antioxidant, antibacterial and antiproliferative activities. Phenolics were extracted using 80% aqueous solvents containing methanol, acidic methanol, acetone and acidic acetone. Our analysis revealed that the acidic acetone extracts recovered the highest level of total phenolics (899mg GAE/100g FW) and flavonoids (433.5mg CE/100g FW). Free radical scavenging activities (DPPH, ABTS, superoxide and linoleate hydroperoxide radicals) and ferric reducing activity were highest in the acetone and acidic acetone extracts. No metal chelating or antibacterial activity was detected in any of the extracts. Acetone and methanol extracts showed potent antiproliferative activity against human cervical cancer cells (C33A) with an EC50 of inhibition at 5.04 and 4. 9mg/ml fruit concentration respectively, while showing no cytotoxicity to normal PBMCs cells. Therefore, the present study concluded that the yellow Himalayan raspberry is a potent source of phytochemicals having super antioxidant and potent antiproliferative activities.


PubMed | Modern Institute of Technology MIT
Type: Journal Article | Journal: Journal of environmental sciences (China) | Year: 2011

A new bacterial strain DMT-7 capable of selectively desulfurizing dibenzothiophene (DBT) was isolated from diesel contaminated soil. The DMT-7 was characterized and identified as Lysinibacillus sphaericus DMT-7 (NCBI GenBank Accession No. GQ496620) using 16S rDNA gene sequence analysis. The desulfurized product of DBT, 2-hydroxybiphenyl (2HBP), was identified and confirmed by high performance liquid chromatography analysis and gas chromatography-mass spectroscopy analysis respectively. The desulfurization kinetics revealed that DMT-7 started desulfurization of DBT into 2HBP after the lag phase of 24 hr, exponentially increasing the accumulation of 2HBP up to 15 days leading to approximately 60% desulfurization of the DBT. However, further growth resulted into DBT degradation. The induced culture of DMT-7 showed shorter lag phase of 6 hr and early onset of stationary phase within 10 days for desulfurization as compared to that of non-induced culture clearly indicating the inducibility of the desulfurization pathway of DMT-7. In addition, Lysinibacillus sphaericus DMT-7 also possess the ability to utilize broad range of substrates as sole source of sulfur such as benzothiophene, 3,4-benzo DBT, 4,6-dimethyl DBT, and 4,6-dibutyl DBT. Therefore, Lysinibacillus sphaericus DMT-7 could serve as model system for efficient biodesulfurization of diesel and petrol.


PubMed | Modern Institute of Technology MIT
Type: Journal Article | Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] | Year: 2013

Benzo [a] Pyrene (BaP) is a highly recalcitrant, polycyclic aromatic hydrocarbon (PAH) with high genotoxicity and carcinogenicity. It is formed and released into the environment due to incomplete combustion of fossil fuel and various anthropogenic activities including cigarette smoke and automobile exhausts. The aim of present study is to isolate bacteria which can degrade BaP as a sole source of carbon and energy. We have isolated a novel strain BMT4i (MTCC 9447) of Bacillus subtilis from automobile contaminated soil using BaP (50 g /ml) as the sole source of carbon and energy in basal salt mineral (BSM) medium. The growth kinetics of BMT4i was studied using CFU method which revealed that BMT4i is able to survive in BaP-BSM medium up to 40 days attaining its peak growth (10(29) fold increase in cell number) on 7 days of incubation. The BaP degradation kinetics of BMT4i was studied using High Performance Liquid Chromatography (HPLC) analysis of BaP biodegradation products. BMT4i started degrading BaP after 24 hours and continued up to 28 days achieving maximum degradation of approximately 84.66 %. The above findings inferred that BMT4i is a very efficient degrader of BaP. To our best of knowledge, this is the first report showing utilization of BaP as a sole source of carbon and energy by bacteria. In addition, BMT4i can degrade a wide range of PAHs including naphthalene, anthracene, and dibenzothiophene therefore, it could serve as a better candidate for bioremediation of PAHs contaminated sites.

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