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Bala M.,Mn Institute Of Applied Science | Nag T.N.,Mn Institute Of Applied Science | Kumar S.,DRMR | Vyas M.,Mn Institute Of Applied Science | And 2 more authors.
JAOCS, Journal of the American Oil Chemists' Society | Year: 2011

The chemical characteristics of Pongamia pinnata seeds, focussing on proximate composition and the fatty acid profile of its oil, are presented. The proximate composition of P. pinnata seeds was: 3.8% ash, 9.7% sugar, 7.07% protein, 24% oil, 10.7% free amino acids, and 0.24% free fatty acids. The oil was extracted from seeds by use of different solvents and the highest yield (29%) was obtained by use of n-hexane. Monounsaturated and polyunsaturated fatty acids accounted for 63.3 and 22.9%, respectively, of the seed oil. Oleic acid was the major fatty acid but a substantial amount of erucic acid was also detected; this was not reported in previous studies. The level of erucic acid and the presence of toxic flavonoids, for example karanjin, pongapin, and pongaglabrin, render the oil inedible according to WHO recommendations. However, low levels of saturated and polyunsaturated fatty acids with desirable cetane number and iodine value suggest potential for application as a biodiesel fuel. © 2010 AOCS.

Sharma A.,Mn Institute Of Applied Science | Singh S.,Mn Institute Of Applied Science | Nag T.N.,Mn Institute Of Applied Science
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2010

Antibacterial activity of Citrullus colocynthis and Tribulus terrestris (whole plant and fruit) extracts prepared in various solvents were studied against some pathogenic bacteria i.e. Bacillus cents, Escherichia coli, Mycobacterium smegmatis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and, S. epidermidis. Disc diffusion method was adopted to screen antibacterial activity and broth dilution method was used to determine Minimum Inhibitory Concentration (MIC). Selected plant extracts exhibited broader and moderate MIC within the range of 20-100ug/mL against all the bacterial pathogens. © Global Science Publications.

Bala M.,Mn Institute Of Applied Science | Nag T.N.,Mn Institute Of Applied Science | Mathur K.,Mn Institute Of Applied Science | Kumar S.,DRMR | And 3 more authors.
Romanian Biotechnological Letters | Year: 2010

From 8-10 days old seedlings of Pisum sativum L. (variety AP-1), various parts root, cotyledon as well as leaf were separated to use as explants. Root and cotyledonary explants did not show any response on MS (Murashige & Skoog) media supplemented with 2, 4-D at a concentration of 5 ppm. However, leaf explants showed callus formation on this media. The callus so formed was subcultured on MS media supplemented with 2, 4-D, IAA, and kinetin. After eight weeks, callus (green and friable) was harvested and used for extraction of lectin protein, known for a wide variety of biological activities in plants, animals and microbes. Seeds were also used to extract lectin protein. Seed extract showed haemagglutination activity of 800 HU/ml, where as callus extract showed 100HU/ml. In seed extract, specific haemagglutination activity was 4 times more than callus extract. Moreover, lectin from seed extract was found to be more thermostable than that of callus extract. In pH range of 6.0-8.0, no loss of lectin activity was observed in seed as well as callus extract. Above and below this pH range, lectin activity showed reduction. Among the various tested sugars, seed as well as callus extract showed specificity for fructose, glucose, maltose, mannose, Nacetyl glucosamine and sucrose. © 2010 University of Bucharest.

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