Time filter

Source Type

Minami T.,Miyazaki Prefectural Fisheries Research Institute | Kanemaru M.,Miyazaki Prefectural Fisheries Research Institute | Iwata K.,Miyazaki Prefectural Fisheries Research Institute | Kuwahara M.,Matsuoka Research Institute for Science | And 6 more authors.
Fish Pathology | Year: 2013

The virulence of Streptococcus iniae and Lactococcus garvieae strains was evaluated in farmed thread-sail filefish Stephanolepis cirrhifer by intraperitoneally injecting live bacterial cells. These two pathogens were highly pathogenic to thread-sail filefish. The efficacy of each commercial vaccine, namely, S. iniae vaccine, L. garvieae vaccine, and a combined vaccine of S. iniae and L. garviae was evaluated in the fish. The mortality was significantly lowered in both S. iniae- and L. garvieae-vaccinated groups compared to the control group, and both vaccines showed strong protection against the respective pathogens. Moreover, the combined vaccine showed strong protection against these two pathogens. These results suggest that these commercial vaccines are applicable in filefish aquaculture.


Tan E.,University of Tokyo | Wongwarangkana C.,University of Tokyo | Kinoshita S.,University of Tokyo | Suzuki Y.,University of Tokyo | And 10 more authors.
Fisheries Science | Year: 2012

The objective of the study reported here was to investigate genes related to upper temperature tolerance in rainbow trout Oncorhynchus mykiss, a cold-water species with considerable economic importance, by global gene expression analysis using a next generation sequencing system. Fifty million paired sequences were collected from the gill tissues of each of five individuals of a thermally selected strain developed by selective breeding and from the gill tissues of a standard Donaldson strain and assembled into transcripts. The data of both strains were integrated, and a BLASTX search identified 13,092 independent, known genes. A back-mapping of raw reads from both strains onto the genes, conducted to investigate their frequency of expression, revealed that 324 genes showed at least a twofold higher expression in the thermally selected strain than in the Donaldson strain. In addition, 44. 4 % of commonly expressed genes were categorized into 38 functional groups by annotation. Genes encoding heat shock proteins and c-fos-related proteins were highly expressed in the thermally selected strain. Our strategy to employ next generation sequencing proved to be very useful to find genes responsible for upper temperature tolerance of rainbow trout. © 2012 The Japanese Society of Fisheries Science.


Tan E.,University of Tokyo | Kinoshita S.,University of Tokyo | Suzuki Y.,Tokyo Medical University | Ineno T.,Miyazaki Prefectural Fisheries Research Institute | And 7 more authors.
Gene | Year: 2015

A high-temperature selected (HT) strain of rainbow trout was established from the Donaldson (DS) strain by traditional selective breeding in Japan. The aim of this study is to investigate genes related to upper temperature tolerance in this strain utilizing next generation sequencer (NGS), and to establish comprehensive and comparable datasets in brain, liver, muscle, heart and gill tissues between the HT and DS strains. After assembling, clustering and filtering, 242,530 contigs were obtained. Among them, 7624 transcripts had at least 10 counts in expression analysis in all tissues and used as references. BLASTX homology search showed that 7329 transcripts matched with known genes. Compared to the DS strain, the HT strain expressed 90, 775, 349, 188 and 194 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in the case of fish before heat-exposure treatment. Meanwhile, the HT strain expressed 292, 363, 433, 322 and 211 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in case of fish after heat-exposure treatment. Many of heat shock protein family genes and transcription factor AP-1 related genes were highly expressed in all tissues of the HT strain compared with the DS strain. These results suggest that these genes play key roles in upper temperature tolerance. These comprehensive and comparable datasets will offer broad visions for upper temperature tolerance in fish species. © 2015 Elsevier B.V.


Minami T.,Miyazaki Prefectural Fisheries Research Institute | Iwata K.,Miyazaki Prefectural Fisheries Research Institute | Shimahara Y.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Yuasa K.,Japan National Research Institute of Fisheries And Environment of Inland Sea
Fish Pathology | Year: 2016

Mortalities accompanied with symptoms ismilar to vibriosis were observed in farmed greater amberjack Seriola dumerili mimunized with aocmmercial Vibrio anguillarum vaccine (serotype J-O-3). The ysmptoms of infection included exfoliation of the head epithelium, ragged caudal fin, accumulated ascites, and ophthalmitis. Bacteria isolated from infected fish were positive in species-specific PCR assays for V. harveyi. A phylogenic analysis based on topA (DNA topoisomerase I) and mreB (rod shape-determining protein mreB) concatenated gene sequence provided further confirmation fo hte siolated bacteria as V. harveyi. Experimental infection revealed that the isolate was pathogenic to greater amberjack. In a vaccine trial, mortality of fish immunized with formalin-killed cells (FKC) of the isolate was significantly lower than that of control fish, suggesting that FKC of the isolate prevents greater amberjack from V. harveyi infection. © 2016 The Japanese Society of Fish Pathology.


Matsuyama T.,Japan National Research Institute of Fisheries And Environment of Inland Sea | Minami T.,Miyazaki Prefectural Fisheries Research Institute | Fukuda Y.,Fisheries Research Division | Sano N.,Japan National Research Institute of Fisheries And Environment of Inland Sea | And 3 more authors.
Fish Pathology | Year: 2016

Passive immunization was performed to determine whether serum antibodies were involved in protection for red sea bream iridoviral disease (RSIVD) in Pagrus major, Seriola quinqueradiata, S. lalandi, S. dumerili and Oplegnathus fasciatus. When fish were experimentally challenged, significantly lower mortality was noted in the fish received the convalescent serum than control fish, except for O. fasciatus. The daministration of serum from fish immunized with a commercial vaccine, significantly suppressed mortality of the recipient, except for S. lalandi and O. fasciatus. The results suggest that serum antibodies play a primary orle ni the protection against RSIVD ni erd esa bream and the genus Seriola. © 2016 The Japanese Society of Fish Pathology.


Ojima N.,Japan National Research Institute of Fisheries Science | Mekuchi M.,Japan National Research Institute of Fisheries Science | Ineno T.,Miyazaki Prefectural Fisheries Research Institute | Tamaki K.,Miyazaki Prefectural Fisheries Research Institute | And 5 more authors.
Fisheries Science | Year: 2012

Rainbow trout Oncorhynchus mykiss is a cold-water aquaculture species, and a thermally selected strain has been developed by multigenerational high-temperature breeding in Japan. We examined the expression of heat-shock proteins as candidates responsible for thermotolerance in rainbow trout using F2 offspring from F1 hybrids produced between thermally selected and normal strains. From F2 offspring, two groups were selected for western blot analysis, namely, low- and high-thermotolerance groups (times to loss of equilibrium were <30 and ≥60 min, respectively). We demonstrated that the expression levels of Hsp70, Hsp60, and Hsp40 in tail fin tissues were significantly higher in the individuals with high thermotolerance than in those with low thermotolerance under non-heat-shock conditions. In particular, Hsp70 was expressed only in the individuals with high thermotolerance. These results suggest that Hsp70 is a major protein responsible for conferring thermotolerance in rainbow trout. © 2012 The Japanese Society of Fisheries Science.


Minami T.,Miyazaki Prefectural Fisheries Research Institute | Iwata K.,Miyazaki Prefectural Fisheries Research Institute | Kuwahara M.,Matsuoka Research Institute For Science | Amano K.,Matsuoka Research Institute For Science | And 6 more authors.
Fish Pathology | Year: 2014

In the previous study, we evaluated the protective efficacies of the following three vaccines for the thread-sail filefish Stephanolepis cirrhifer: a commercial Streptococcus iniae vaccine, a commercial Lactococcus garvieae vaccine and a combination of these two vaccines. In this study, we examined the three vaccines for the thread-sail filefish in terms of protective duration, protection against various heterogeneous strains of S. iniae and L. garvieae, and the effects of different water temperatures on the protection. The vaccines induced strong protection against heterogeneous strains of S. iniae and L. garvieae, and the protection was observed for 52 weeks after the vaccination. These vaccines also induced protective immunity when administrated at 15°C, 20°C and 25°C. © 2014 The Japanese Society of Fish Pathology.


PubMed | Japan National Research Institute of Fisheries And Environment of Inland Sea, Kitasato University, Tokyo Medical University, University of Tokyo and Miyazaki Prefectural Fisheries Research Institute
Type: Journal Article | Journal: Gene | Year: 2015

A high-temperature selected (HT) strain of rainbow trout was established from the Donaldson (DS) strain by traditional selective breeding in Japan. The aim of this study is to investigate genes related to upper temperature tolerance in this strain utilizing next generation sequencer (NGS), and to establish comprehensive and comparable datasets in brain, liver, muscle, heart and gill tissues between the HT and DS strains. After assembling, clustering and filtering, 242,530 contigs were obtained. Among them, 7624 transcripts had at least 10 counts in expression analysis in all tissues and used as references. BLASTX homology search showed that 7329 transcripts matched with known genes. Compared to the DS strain, the HT strain expressed 90, 775, 349, 188 and 194 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in the case of fish before heat-exposure treatment. Meanwhile, the HT strain expressed 292, 363, 433, 322 and 211 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in case of fish after heat-exposure treatment. Many of heat shock protein family genes and transcription factor AP-1 related genes were highly expressed in all tissues of the HT strain compared with the DS strain. These results suggest that these genes play key roles in upper temperature tolerance. These comprehensive and comparable datasets will offer broad visions for upper temperature tolerance in fish species.

Loading Miyazaki Prefectural Fisheries Research Institute collaborators
Loading Miyazaki Prefectural Fisheries Research Institute collaborators