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Ando A.,Tokai University | Shigenari A.,Tokai University | Ota M.,Shinshu University | Sada M.,Japan National Cardiovascular Center Research Institute | And 9 more authors.
Tissue Antigens | Year: 2011

A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci. © 2011 John Wiley & Sons A/S. Source


Okamura T.,Tohoku University | Okamura T.,National Institute of Livestock and Grassland Science | Onodera W.,Tohoku University | Tayama T.,Tohoku University | And 11 more authors.
Animal Genetics | Year: 2012

Respiratory disease is the most important health concern for the swine industry. Genetic improvement for disease resistance is challenging because of the difficulty in obtaining good phenotypes related with disease resistance; however, identification of genes or markers associated with disease resistance can help in the genetic improvement of pig health. The purpose of our study was to investigate whether quantitative trait loci (QTL) associated with disease resistance were segregated in a purebred population of Landrace pigs that had been selected for meat production traits and mycoplasmal pneumonia of swine (MPS) scores over five generations. We analysed 1395 pigs from the base to the fifth generation of this population. Two respiratory disease traits [MPS scores and atrophic rhinitis (AR) scores] and 11 immune-capacity traits were measured in 630-1332 animals at 7 weeks of age and when the animal's body weight reached 105 kg. Each of the pigs, except sires in the base population, was genotyped using 109 microsatellite markers, and then, QTL analysis of the full-sib family population with a multi-generational pedigree structure was performed. Variance component analysis was used to detect QTL associated with MPS or AR scores, and the logarithm of odds (LOD) score and genotypic heritability of the QTL were estimated. Five significant (LOD > 2.51) and 18 suggestive (LOD > 1.35) QTL for respiratory disease traits and immune-capacity traits were detected. The significant QTL for Log-MPS score, located on S. scrofa chromosome 2, could explain 87% of the genetic variance of this score in this analysis. This is the first report of QTL associated with respiratory disease lesions. © 2012 The Authors, Animal Genetics © 2012 Stichting International Foundation for Animal Genetics. Source


Kadowaki H.,Miyagi Livestock Experimental Station | Suzuki E.,Miyagi Livestock Experimental Station | Kojima-Shibata C.,Miyagi Livestock Experimental Station | Suzuki K.,Tohoku University | And 4 more authors.
Livestock Science | Year: 2012

The objective of this experiment was to evaluate the selection of a pig line with improved resistance to swine mycoplasmal pneumonia and meat production in Landrace pigs. The selection was conducted over 5 generations using estimated breeding values of daily gain (DG), backfat thickness (BF), and mycoplasmal pneumonia scores (MPS). In order to evaluate various lung lesions, we established two different rearing environments (regularly cleaned vs. not cleaned) for the pigs. The heritability estimates for DG, BF, and MPS were found to be 0.65, 0.60, and 0.07, respectively. To compensate for the low heritability of MPS we examined the genetic correlation of immunity traits with MPS to determine their usefulness in a selection index. Cortisol (COR) plasma levels at 105. kg body weight had a high positive genetic correlation (0.65) with MPS, and therefore used as an immunity trait. The average breeding values in the fifth generation, as expressed in standard deviation units, were 1.32 for DG, -0.70 for MPS, and -0.04 for BF and COR. The selection traits showed good genetic progress in 5 generations demonstrating the possibility of selecting for both disease resistance and high meat production in animals. © 2012. Source

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