Lund, Sweden
Lund, Sweden

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Grant
Agency: European Commission | Branch: FP7 | Program: MC-ITN | Phase: FP7-PEOPLE-2010-ITN | Award Amount: 3.05M | Year: 2012

PEPMIP represents a joint European effort involving eleven partners aimed at the development of the next generation of dedicated separation materials, designed to recognize peptides and proteins, and the implementation of these materials in new high performance methods for peptide and protein analysis. Artificial receptors will be developed by various Molecular Imprinting techniques. This will be supplemented by a new class of generic peptide and protein fractionation tools that will be integrated in new formats to produce new protein/peptide separation and detection solutions. The research results will lead to technological advances having a major impact on 1) health care since it will profit from methods involving PEPMIPs for earlier, more reliable diagnosis of diseases, 2) drug discovery allowing a faster target or biomarker identification; and 3) biochemistry research laboratories in resulting in improved protein fractionation tools for revealing low abundant post translational modifications. The training will focus on 10 early stage researchers (ESRs) who, within four work packages, will develop a well-balanced spectrum of scientific, business and entrepreneurial skills that will be particularly attractive to European industry when the ESRs eventually leave PEPMIP.


Martinez Bueno M.J.,University of Almeria | Herrera S.,University of Almeria | Ucles A.,University of Almeria | Aguera A.,University of Almeria | And 6 more authors.
Analytica Chimica Acta | Year: 2010

This paper describes the development of an analytical procedure to determine malachite green (MG) residues in salmon samples using molecularly imprinted polymers (MIPs) as the extraction and clean-up material, followed by liquid chromatography-linear ion trap mass spectrometry (LC-QqQLIT-MS/MS). MG and two structurally related compounds, crystal violet (CV) and brilliant green (BG) were employed for the selectivity test. The imprinted polymers exhibited high binding affinity for MG, while CV and BG showed less binding capacity: 47% and 34%, respectively. The recovery values of MG in salmon samples fortified with leucomalachite green (LMG) were determined by measuring the amount of MG in the sample, after carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which converts the LMG back into chromic-form. The average recovery of MG in spiked salmon muscle over the concentration range 1-100ngg-1 was 98% with a relative standard deviation value (R.S.D.) below 12%. The method detection limits (MDLs) obtained for MG, CV, BG and their leuco-metabolites were in the range of 3-20ngkg-1 (ppt). © 2010 Elsevier B.V.


Kecili R.,MIP Technologies AB | Billing J.,MIP Technologies AB | Nivhede D.,MIP Technologies AB | Sellergren B.,Malmö University | And 2 more authors.
Journal of Chromatography A | Year: 2014

This study describes the identification and evaluation of molecularly imprinted polymers (MIPs) for the selective removal of potentially genotoxic aminopyridine impurities from pharmaceuticals. Screening experiments were performed using existing MIP resin libraries to identify resins selective towards those impurities in the presence of model pharmaceutical compounds. A hit resin with a considerable imprinting effect was found in the screening and upon further investigation, the resin was found to show a broad selectivity towards five different aminopyridines in the presence of the two model active pharmaceutical ingredients (APIs) piroxicam and tenoxicam. © 2014 Elsevier B.V.


Kecili R.,MIP Technologies AB | Kecili R.,TU Dortmund | Billing J.,MIP Technologies AB | Leeman M.,MIP Technologies AB | And 4 more authors.
Separation and Purification Technology | Year: 2013

The present study describes an easy and efficient approach for the removal of methyl p-toluenesulfonate, a potentially genotoxic impurity (GTI) from a model active pharmaceutical ingredient (API), 21-chlorodiflorasone. For this purpose, several polystyrene-divinylbenzene based and silica based scavengers were tested and the scavengers which showed the highest removal efficiency and selectivity were chosen for further evaluations. The time course of GTI and API scavenging and also the capacities of different scavengers were studied in this paper. The best GTI removal performance and selectivity was obtained with silica based Si-Trisamine and macroporous polystyrene-divinylbenzene based MP-Trisamine, both leading to 100% methyl p-toluenesulfonate removal with no loss of 21-chlorodiflorasone. Non-specifically bound API could be recovered quantitatively by using 2-propanol:THF (1:1). © 2012 Published by Elsevier B.V. All rights reserved.


Chimuka L.,University of Witwatersrand | van Pinxteren M.,Helmholtz Center for Environmental Research | Billing J.,MIP Technologies AB | Yilmaz E.,MIP Technologies AB | Jonsson J.T.,Lund University
Journal of Chromatography A | Year: 2011

A selective extraction technique based on the combination of membrane assisted solvent extraction and molecularly imprinted solid phase extraction for triazine herbicides in food samples was developed. Simazine, atrazine, prometon, terbumeton, terbuthylazine and prometryn were extracted from aqueous food samples into a hydrophobic polypropylene membrane bag containing 1000 μL of toluene as the acceptor phase along with 100. mg of MIP particles. In the acceptor phase, the compounds were re-extracted onto MIP particles. The extraction technique was optimised for the type of organic acceptor solvent, amount of molecularly imprinted polymers particles in the organic acceptor phase, extraction time and addition of salt. Toluene as the acceptor phase was found to give higher triazine binding onto MIP particles compared to hexane and cyclohexane. Extraction time of 120. min and 100. mg of MIP were found to be optimum parameters. Addition of salt increased the extraction efficiency for more polar triazines. The selectivity of the technique was demonstrated by extracting spiked cow pea and corn extracts where clean chromatograms were obtained compared to only membrane assisted solvent extraction or only molecularly imprinted solid phase extraction. The study revealed that this combination may be a simple way of selectively extracting compounds in complex samples. © 2010 Elsevier B.V.


Kecili R.,MIP Technologies AB | Kecili R.,TU Dortmund | Nivhede D.,MIP Technologies AB | Billing J.,MIP Technologies AB | And 3 more authors.
Organic Process Research and Development | Year: 2012

A variety of chemical compounds, intermediates, and reagents are used during the process of synthesizing active pharmaceutical ingredients (APIs). Some of these chemicals, intermediates, and reagents, as well as byproducts of synthetic processes, can have toxic properties and be present as impurities at low levels in the API or final drug formulation. If present at high concentrations, the toxic impurities could cause adverse health effects in humans. This paper describes a simple and rapid approach for selective removal of acrolein from APIs using iodixanol as a model API. Several scavengers were tested, and the resins which showed highest binding efficiency and selectivity were chosen for further evaluations. The kinetics of acrolein scavenging in the presence of the API iodixanol and the scavenging capacity of resins were demonstrated in this paper. The most complete scavenging is obtained with PS-NH 2 which removes 97.8% of acrolein without any substantial removal of the API during 20 min of reaction time. © 2012 American Chemical Society.


Grant
Agency: European Commission | Branch: FP7 | Program: MC-ITN | Phase: PEOPLE-2007-1-1-ITN | Award Amount: 2.94M | Year: 2008

The NEMOPUR Initial Training Network will focus on creating a new generation of molecular purification technologies for Active Pharmaceutical Ingredient (API) manufacture, with a particular focus on removal of organic impurities from API production. New membranes with improved chemical stability in a wide range of solvents, and better controlled cutoff properties, novel molecularly imprinted polymers with readily scalable production chemistries, and composite molecularly imprinted film macroporous support membranes will be developed. The applications of these technologies will be through the industrial partners. The project is multidisciplinary, involving chemists, materials scientists and chemical engineers. The consortium is intersectorial, comprising 3 universities/research institutes, 2 technology SMEs and 4 pharmaceutical manufacturers, and will have a strong emphasis on knowledge creation, technology commercialisation, and entrepreneurship. The training programme involves Early Stage Researchers (ESR), at both university and industrial partners, each of whom will complete a PhD thesis through a combination of local and network wide research experience. Experienced Researchers (ER) will complete their training, focussing on knowledge transfer, commercialisation and entreprenuership skills. In addition to their individual research, ESR and ER will complete complementary training through a series of Personal Skills Modules, and a course on Technology Commercialisation and Entrepreurship. NEMOPUR seeks to contribute to improving the European knowledge supply chain through this industry-academia programme of applied research aimed at developing engineers and scientists who are academically excellent and achieve PhD degrees, but who thrive at the interface between fundamental research and industrial application.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: NMP-2008-1.2-2 | Award Amount: 3.31M | Year: 2009

The present WATERMIM proposal is focused on the advancement and optimization of the MIP technology in order to produce functional materials with well-defined morphologies with respect to pore structure and selectivity for water treatment applications. The project aims at the elimination of the random distribution and the uneven accessibility of receptor sites in the volume of the imprinted material that is crucial for its performance. Such novel materials will immediately gain practical relevance, especially, due to their increased selectivity and superior stability under long and harsh technical conditions. The simultaneous optimization of the imprinting efficiency, polymer membrane morphology and separation conditions will enable the development of a truly molecular selective water purification process, based on affinity interactions that would have a large application impact on the water treatment industry. All types of synthetic organic compounds (i.e., triazines, pharmaceutical compounds and endocrine disruptors) are considered target compounds in the WATERMIM project. More specifically, the present project aims at the following S&T objectives: Selection of template molecules and synthesis of functional monomers. Optimization of molecularly imprinted polymer (MIP) composition by computational design techniques and combinatorial screening. Synthesis of well-defined MIP nanoparticles and microgels. Production of novel composite membranes utilizing preformed MIP nanoparticles. Production of composite filters both on organic and inorganic supports via novel grafting techniques. Synthesis of molecularly imprinted membranes (MIMs) for molecular sensor applications. Separation and catalytic decomposition of the pollutants. Advanced monitoring of the target compounds. Benchmark testing of the produced MIMs for water purification.


Duarte M.,MIP Technologies AB | Billing J.,MIP Technologies AB | Yilmaz E.,MIP Technologies AB
Journal of Applied Polymer Science | Year: 2016

Molecularly imprinted polymers (MIPs) have been synthesized in the absence of a solvent using fumed silica nanoparticles to create a porous network. The method employed led to a chiral imprinting effect and allowed for an excellent control over the internal morphology of imprinted and non-imprinted polymer (NIP) materials. The polymers possess high surface areas (>300 m2) and identical pore size (112 Å). The MIP exhibited an imprinting factor (IF) of 9 and a selectivity value (α) of 1.83 for (-)-ephedrine. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44104. © 2016 Wiley Periodicals, Inc.


Leeman M.,MIP Technologies AB | Santacruz S.,San Francisco de Quito University
Analytical and Bioanalytical Chemistry | Year: 2011

Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min-1 and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 106 g mol-1, and most of the second size population had molar masses above 107 g mol-1. Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties. © 2010 Springer-Verlag.

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