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Zhu H.-Q.,Peoples Hospital of Guangxi Autonomous Region | Liu Y.,Guangxi Medical University | Chen F.,Peoples Hospital of Guangxi Autonomous Region | Liu D.-H.,Minzu Hospital of Guangxi Zhuang Autonomous Region
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Patellar tendon plays a very important role in the movement of knee joint. Patellar tendon rupture is relatively rare in clinic, so the misdiagnosis rate is high. There are many ways for patellar ligament reconstruction and the therapeutic effect is very different. Improper treatment can lead to patella malposition that would seriously affect the function of the knee joint. OBJECCTIVE: To explore the clinical efficacy of autologous tendon graft with preserved distal insertions on the treatment of old patellar tendon rupture. METHODS: Eight patients with unilateral old patellar tendon rupture underwent reconstruction using semitendinosus-gracilis tendons with preserved distal insertions. After patellar ligament reconstruction, semi-circular gypsum was used for fixation and the knee joint was kept in flexion of 15° for 1 week. At 2 weeks after patellar ligament reconstruction, surgical incision suture was removed, and 2 weeks later, gypsum was removed for quadriceps isometric and isotonic exercises. At 6 weeks after reconstruction, adjustable knee brace was used for the range of motion exercise, three times per day, and the flexion angle was increased for 10° every week. The patients had fully weight-bearing walking at 3 months after patellar ligament reconstruction. RESULTS AND CONCLUSION: All patients had no complications after patellar ligament reconstruction, and X-ray film showed that the patella returned to normal height. Function of knee joint had generally returned to normal. No patient had knee discomfort after walking for two kilometers in 24 months. Compared with that before patellar ligament reconstruction, Lysholm score of the patients at 24 months after reconstruction was increased significantly (P < 0.05). The results indicate that semitendinosus-gracilis tendons with preserved distal insertions used for patellar ligament reconstruction can reconstruct and treat old patellar tendon rupture. Source

Peng F.,Wuhan University | Wu H.,Huazhong University of Science and Technology | Zheng Y.,Minzu Hospital of Guangxi Zhuang Autonomous Region | Xu X.,Huazhong University of Science and Technology | Yu J.,Huazhong University of Science and Technology
Lasers in Medical Science | Year: 2012

Mesenchymal stem cells (MSCs) are promising for use in regenerative medicine. Low-level light irradiation (LLLI) has been shown to modulate various processes in different biological systems. The aim of our study was to investigate the effect of red light emitted from a lightemitting diode (LED) on bone marrow MSCs with or without osteogenic supplements. MSCs both with and without osteogenic supplements were divided into four groups, and each group was irradiated at doses of 0, 1, 2 and 4 J/cm 2. Cellular proliferation was evaluated using WST-8 and 5-ethynyl-2'-deoxyuridine (EdU) fluorescence staining. The alkaline phosphatase activity, mineralization, and expression of osteoblast master genes (Collαl, Alpl, Bglap and Runx2) were monitored as indicators of MSC differentiation towards osteoblasts. In groups without osteogenic supplements, red light at all doses significantly stimulated cellular proliferation, whereas the osteogenic phenotype of the MSCs was not enhanced. In groups with osteogenic supplements, red light increased alkaline phosphatase activity and mineralized nodule formation, and stimulated the expression of Bglap and Runx2, but decreased cellular proliferation. In conclusion, nonconherent red light can promote proliferation but cannot induce osteogenic differentiation of MSCs in normal media, while it enhances osteogenic differentiation and decreases proliferation of MSCs in media with osteogenic supplements. © Springer-Verlag London Ltd 2011. Source

Zou W.,Minzu Hospital of Guangxi Zhuang Autonomous Region
Nan fang yi ke da xue xue bao = Journal of Southern Medical University | Year: 2013

In patients with diabetes, glucose fluctuations, insulin resistance, poor circulation, and likely immune damage can easily lead to infections. Splenic abscess is rare in diabetic patients and is associated with a high mortality rate. Type 2 diabetes causes increased risks of splenic abscess, and timely and effective treatment can lower the mortality rate. We report here a case of type 2 diabetes complicated by multiple splenic abscesses. Source

Xiang Y.H.,Minzu Hospital of Guangxi Zhuang Autonomous Region
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2012

To Investigate the influences of chronic intermittent hypoxia (CIH) and continuous hypoxia (CH) on renin angiotensin system (RAS) in serum and tissues of rats, and therefore to investigate the mechanism of CIH-induced hypertension and hypoxia induced pulmonary hypertension. Eighteen male Sprague-Dawley (SD) rats were divided into 3 groups: CIH group, CH group and control group (UC). CIH rats were subjected to alternating cycles of hypoxia (6% ∼ 8% O(2) in N(2) for 20 ∼ 25 s) and normoxia (21% O(2) in N(2) for 2 min) every 180 s for 7 h/d. CH rats were consistently given nitrogen (oxygen concentration 8% - 12% in the cabin, 7 h/d), while the UC rats were not treated. Systolic blood pressure (SBP) in the CIH rats at the end of 6th week was significantly elevated compared with baseline SBP (P < 0.001), and that in the CH and the UC rats (P < 0.05). At the end of 6th week, the expression of ACE and ACE2 in the renal arteriole was significantly different (P < 0.05), and the levels of AngII in serum and kidney tissues were increased. Ang-(1-7) was decreased in the CIH rats compared with the CH and the UC rats (P < 0.05). The levels of AngII in pulmonary tissues were increased, while the levels of Ang-(1-7) were decreased in the CH rats compared with the CIH and the UC rats (P < 0.05). SBP showed a positive correlation with AngII in serum and kidney tissues, and a negative correlation with Ang-(1-7) in serum and kidney tissues. There were significant differences in arterial wall thickness, WT%, and WA% of renal arterioles and pulmonary arterioles among the 3 groups. Wall thickness of pulmonary arterioles and kidney arterioles was positively correlated with AngII in pulmonary and kidney tissues (r = 0.386, 0.414, P < 0.05), and negatively correlated with Ang-(1-7) (r = -0.401, -0.394, P < 0.05). CIH and CH showed different effects on RAS in the serum and the tissues of rats. CIH mainly affected levels of RAS in the serum, kidney tissues and renal arterioles, and was closely related with blood pressure. CH mainly affected the levels of RAS in lung tissues and pulmonary small arteries, which may be related with pulmonary, hypertension and pulmonary arterial remodeling. Source

Liu T.,Guangxi Medical University | Zhou Y.,Minzu Hospital of Guangxi Zhuang Autonomous Region | Wang J.-Y.,Wuhan Asia Heart Hospital | Su Q.,Guangxi Medical University | And 3 more authors.
Journal of Cardiovascular Pharmacology and Therapeutics | Year: 2016

Background: Cardiomyocyte apoptosis by coronary microembolization (CME) contributes to myocardial dysfunction, in which mitochondrial pathway and death receptor pathway are activated. Lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) is a membrane protein involved in apoptosis. The study aimed to explore the role of LOX-1 in the activation of these 2 major apoptotic pathways. Methods: Twenty Bama miniature swine were randomized into 4 groups (n = 5 per group). The groups were Sham, CME, LOX-1 small-interfering RNA (siRNA), and control siRNA. Microspheres were injected into the left anterior descending artery of swine to establish CME model. Twelve hours after operation, cardiac function, serum c-troponin I level, microinfarct, and apoptotic index were examined. The levels of LOX-1, Bcl-2, Bax, cytochrome c as well as cleaved caspase 9, -8, and -3 were detected. Results: Myocardial dysfunction, enhanced serum c-troponin I, microinfarct, and apoptosis were induced following CME. Moreover, CME induced increased expression of LOX-1, Bax, cytochrome c, cleaved caspase 9, -8, and -3 as well as decreased Bcl-2 expression levels. The LOX-1 siRNA reversed these effects by CME except cleaved caspase 8 expression, while the control siRNA had no effect. Conclusion: Coronary microembolization induces cardiomyocyte apoptosis via the LOX-1-dependent mitochondrial pathway and caspase 8-dependent pathway. © The Author(s) 2015. Source

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