VERONA, WI, United States

Minitube Of America

www.minitube.com
VERONA, WI, United States
SEARCH FILTERS
Time filter
Source Type

Didion B.A.,Minitube Of America | Braun G.D.,HyLife Ltd. | Duggan M.V.,Fast Genetics
Animal Reproduction Science | Year: 2013

Worldwide, greater than 90% of sows are inseminated with fresh semen. Less than 1% is inseminated using frozen semen. Albeit, frozen semen is an effective technology for the transfer of genes between breeding pyramids and also to reliably provide semen for planned matings. Little information exists on the long term use of frozen boar semen in commercial pork production operations. The objective in the present study was to assess application of frozen semen throughout a 4 year period comprising more than 2600 AI services. The frozen semen sourced from a boar stud in Manitoba, Canada. All artificial insemination (AI) occurred on a single 1800 sow farm in Indiana, USA. The sperm-rich fraction was collected and only those collections having ≥80% motility and ≤15% abnormalities were further processed. Semen was prepared for cryopreservation using Androhep® CryoGuard™, packaged in 0.5mL French straws (average 500 million total sperm per straw) and frozen using a programmable freezer (IceCube™). For each frozen ejaculate, a post-thaw quality check was performed. Ninety eight percent of the ejaculates that were frozen showed at least a 50% post-thaw motility and were approved for shipment. For AI, eight straws were thawed (to achieve at least 2.0×109motile sperm) and diluted with 60mL of extender pre-warmed to 26°C. Within 2-5min of thawing, the sows or gilts were inseminated via intra-cervical deposition using a standard AI pipette. Sows and gilts were inseminated three times PM/AM/PM and AM/PM/AM, respectively. Of 2696 recorded services, 2122 (78.7%) of the females farrowed. The mean (±SD) total number piglets born were 12.5 (±3.9). A progressive improvement of fertility over time was observed mainly due to adaptive procedures associated with an introduced technology. In summary, acceptable fertility is possible with frozen semen and has merit for application as a reproductive management tool. © 2013 Elsevier B.V.


Trademark
Minitub International Ag, Minitub Gmbh and Minitube Of America | Date: 2013-05-29

Culture, storage and dilution media for maintaining the life and maximizing the viability of animal semen, oocytes, embryos, stem cells and other biological cells for scientific and research use. Culture, storage and dilution media for maintaining the life and maximizing the viability of semen, oocytes, embryos, stem cells and other biological cells for medical and veterinary use; semen extender and storage media used in the artificial insemination process for medical and veterinary use.


Grant
Agency: Department of Health and Human Services | Branch: | Program: STTR | Phase: Phase II | Award Amount: 3.00M | Year: 2012

DESCRIPTION (provided by applicant): Xenotransplantation with pig organs offers the best near term hope for satisfying the limitation imposed by shortage of human organs. This research project is directed toward producing as a product a genetically modified pig that will be the best potential donor for xenotransplants to humans. Although hyperacute rejection of pig organs in primate recipients has been overcome by the production of (-1,3-galactosyltransferase knockout (GalT-KO) pigs, experiments to datewith GalT-KO organs strongly suggest that clinically relevant chronic immunosuppression of recipients will be insufficient to overcome rejection of xenotransplants. Tolerance induction through mixed hematopoietic chimerism is currently the best means for inducing such tolerance, and this methodology has recently been shown to allow human allograft acceptance without chronic immunosuppression. The major problem in achieving mixed chimerism across the pig-to- primate barrier has the difficulty in achieving durable chimerism. Our proposed product will consist of genetically modified pigs with enhanced xenogeneic hematopoietic chimerism potential that we will make available commercially to the research community, thereby providing a strategy for advancing solid organ xenotransplantation toward the clinic. We will achieve this goal by overcoming the species incompatibility of the CD47 (IAP, integrin associated protein) cell surface molecule, which appears to be the key barrier to the establishment of chimerismfollowing pig-to-primate bone marrow transplantation. Ubiquitously expressed, CD47 binds SIRP( receptors on macrophages and thereby inhibits phagocytosis. Expression of primate CD47 on pig cells greatly reduces their susceptibility to phagocytosis by humanmacrophages. In Phase I of this STTR proposal we developed and verified the tools necessary for production, via nuclear transfer, of pigs expressing primate CD47 on a GalT-KO background. We successfully developed targeting/expression vectors for CD47 and molecular assays for identifying targeted cell clones. We have also verified a primary cell clone isolation and have isolated primary fetal fibroblast lines for use in the production of CD47 transgenic pigs. In Phase II, we now intend to use these toolsto produce GalT-KO pigs expressing primate CD47 via nuclear transfer, analyze CD47 expression in these pigs, evaluate the efficacy of CD47 expression using in vitro systems, and perform proof-of-concept transplant experiments to validate the usefulness ofthis product in achieving mixed xenogeneic chimerism and tolerance. PUBLIC HEALTH RELEVANCE: The demand for transplantable human organs far exceeds the current supply and the gap between demand and supply continues to grow. The transplantation of pighearts, kidneys and other organs (known as xenotransplantation) offers the opportunity to greatly reduce or eliminate this shortage. Having now established the necessary tools to produce genetically modified pigs that will facilitate such pig-to-human organ transplants, we now intend to produce these pigs as a commercial product that will further the development of the filed of xenotransplantation.


Trademark
Minitube Of America | Date: 2013-05-29

Culture, storage and dilution media for maintaining the life and maximizing the viability of animal semen, oocytes, embryos, stem cells and other biological cells other than for medical and veterinary use. Culture, storage and dilution media for maintaining the life and maximizing the viability of semen, oocytes, embryos, stem cells and other biological cells for medical and veterinary use; semen extender and storage media used in the artificial insemination process for medical and veterinary use.


Trademark
Minitube Of America | Date: 2013-05-10

Culture, storage and dilution media for maintaining the life and maximizing the viability of animal semen, oocytes, embryos, stem cells and other biological cells. Culture, storage and dilution media for maintaining the life and maximizing the viability of semen, oocytes, embryos, stem cells and other biological cells; semen extender and storage media used in the artificial insemination process; animal semen; biological tissue, namely, stem cells, gametes and embryos for medical or clinical use; living animal organs, namely, hearts, lungs, pancreas, kidneys, livers, skin, and uterus for medical or clinical use as surgical implants and transplants. microfluidic devices, namely, microchannels with and without cross-channels, microchambers, microreactors, filters, columns, mixers, suction ports, separators, valves and pumps, used to store, handle, fertilize, evaluate, culture and identify oocytes and embryos for use in artificial reproduction; general laboratory equipment, namely, microscopes, pipettors, water baths, peristaltic pumps, balances, thermometers, photometers and laboratory ovens; computer software used to analyze, evaluate and store images of sperm cells, oocytes, embryos and other biological cells. Equipment for the collection, processing, packaging and preservation of animal semen; embryo and oocyte recovery, comprising of filters, fluid flow control mechanisms and searching and culture dishes; equipment used in the embryo transfer process for pigs, cattle, horses, dogs, sheep, goats and other animals; equipment for the artificial insemination and fertilization of pigs, cattle, horses, dogs, sheep, goats and other animals, namely, semen collection and storage vessels, semen measurement, evaluation and processing apparatus, semen dispensing instruments, semen dispensing catheters, embryo collection instruments, embryo storage and transfer containers, and other animal husbandry equipment, namely, breeding saddles.


Trademark
Minitube Of America | Date: 2013-03-12

semen extender and storage medium used in the artificial insemination process for long term storage of the semen of animals.


Patent
Minitube Of America | Date: 2013-06-06

A multiple chambered container for biological liquids useful in artificial insemination is disclosed.


A transparent cover is provided for positioning on a slide to observe a fluid situated on the slide. The cover has a cover body which has an upper surface and an opposing lower surface. Portions of the lower surface are parallel to an observation region on the upper surface of the cover body. A sample inlet opening and a vent hole extend through the cover body with the observation region positioned therebetween. At least one rib protrudes downwardly from the lower surface.


Trademark
Minitube Of America | Date: 2013-05-10

Culture, storage and dilution medium for maintaining the life, maximizing the viability, and assisting in gender selection of animal semen, oocytes, embryos, stem cells and other biological cells other than for medical and veterinary use. Culture, storage and dilution medium for maintaining the life, maximizing the viability, and assisting in gender selection of animal semen, oocytes, embryos, stem cells and other biological cells for medical and veterinary use.


Trademark
Minitube Of America | Date: 2014-03-11

Equipment for the artificial insemination and fertilization of animals, namely, bags used to collect, filter, process, store, transport, preserve and dispense animal semen.

Loading Minitube Of America collaborators
Loading Minitube Of America collaborators