Gao S.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Chang G.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Tian J.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Gao S.,China National Institute of Biological Sciences |
Cai T.,China National Institute of Biological Sciences
Genomics Data | Year: 2014
Finding the markers to predict the quality of induced pluripotent stem cells (iPSCs) will accelerate its practical application. The fully pluripotent iPSCs has been determined as viable all-iPSC mice can be generated through tetraploid (4N) complementation. The activation of the imprinted Dlk1-Dio3 gene cluster was reported to correlate with the pluripotency of iPSCs. However, recent studies demonstrated that the loss of imprinting at the Dlk1-Dio3 locus does not strictly correlate with the reduced pluripotency of iPSCs. In our study (ref ), iPSC lines with the same genetic background and proviral integration sites were established, and the pluripotency state of each iPSC line was well characterized using tetraploid (4N) complementation assay. The gene expression and global epigenetic modifications of "4N-ON" and the corresponding "4N-OFF" iPSC lines were compared through deep sequencing analysis of mRNA expression, small RNA profiling, histone modifications (H3K4me3, H3K27me3 and H3K4me2) and DNA methylation. Very few differences were detected in the iPSC lines that were investigated. However, an imprinted gene, Zrsr1 was disrupted in the "4N-OFF" iPSC lines. Here we provide more detail about the dataset and the R script with additional data for others to repeat the finding. © 2014.
Wang B.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Ma W.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Xu X.,Ministry of Agriculture Key Laboratory of Animal Genetics |
Wang C.,Ministry of Agriculture Key Laboratory of Animal Genetics |
And 5 more authors.
Journal of Animal Science and Biotechnology | Year: 2013
Background: The p21-activated kinase 1 (PAK1) is essential for mitosis and plays an important role in the regulation of microtubule assembly during oocyte meiotic maturation in mice; however, little is known about its role in porcine oocytes.Result: Total p21-activated kinase 1 (PAK1) and phosphorylated PAK1 at Thr423 (PAK1Thr423) were consistently expressed in porcine oocytes from the germinal vesicle (GV) to the second metaphase (MII) stages, but phosphorylation of histone H3 at Ser10 (H3Ser10) was only expressed after the GV stage. Immunofluorescence analysis revealed that PAK1Thr423 and H3Ser10 colocalized on chromosomes after the GV stage. Blocking of endogenous PAK1Thr423 by injecting a specific antibody decreased the phosphorylation level of H3Ser10; however, it had no impact on chromatin condensation, meiotic progression, cleavage rate of blastomeres or the rate of blastocyst formation.Conclusion: Phosphorylation of PAK1Thr423 is a spontaneous activation process and the activated PAK1Thr423 can promote the phosphorylation of H3Ser10; however, this pathway is not required for meiotic maturation of porcine oocytes or early embryonic development. © 2013 Wang et al.; licensee BioMed Central Ltd.