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Two local and three imported entomopathogenic nematodes (EPNs) were tested for control of the American cockroach (Periplaneta americana) and the German cockroach (Blattella germanica). Only two Steinernematidae caused substantial cockroach mortality; one was a local strain of Steinernema sp. (strain T1), and the other was an imported strain of S. carpocapsae. A homemade bait that contained cat food and attapulgite clay at a ratio of 3:7 (W:W, 10 g total per bait) and 1x10 6 Steinernema sp. (T1) per bait resulted in 48.0 ± 4.7% mortality of the American cockroach and 57.7 ± 8.0% mortality of the German cockroach. A similar bait containing S. carpocapsae caused 40.0 ± 3.3% mortality of the American cockroach and 86.7 ± 4.7% mortality of the German cockroach. The optimal concentration of Steinernema sp. (T1) and S. carpocapsae to control the American and German cockroach was 1x10 6 EPNs and 5.4x10 4 EPNs per bait, respectively. The most susceptible stage of the American cockroach to both EPNs was the last instar, but susceptibility of the German cockroach to both EPNs did not differ among cockroach stages.


Maketon M.,Kasetsart University | Somsook V.,Ministry of Agriculture and Cooperative | Rattanakorn P.,Kasetsart University | Hotaka D.,Kasetsart University
Nematropica | Year: 2011

An isolate of the entomopathogenic nematode Heterorhabditis indica, designated "T2," was collected in Kanchanaburi Province, Thailand. The life cycle of T2 in Galleria mellonella larvae required 10 days and two generations at 25°C. The first generation was hermaphroditic and the second was amphimictic. Survival of infective-stage juveniles in water-filled sponges at 15°C was 81% after 1 month. In the laboratory, T2 was able to penetrate and kill Aphis gossypii, Spodoptera exigua, Sitophilus zeamais, Tenebrio molitor, Bactrocera correcta, and Coptotermis gestroi but reproduced poorly in all of these hosts except S. exigua. In G. mellonella, T2 reproduced best at 25°C, and the optimum soil moisture for infection was 7 to 9%. Isolate T2 reared on yeast salt broth caused 84% mortality 2 days after inoculation of diamondback moth (Plutella xylostella L.) third-instar larvae. The best artificial medium for mass-rearing H. indica T2 was modified yeast salt broth containing 2.0% soluble starch, 1.5% yeast extract, 2.5% nutrient broth, 1.0% lard, and 2.0% MgCl2 • 6H2O.


Kawtrakul A.,Kasetsart University | Raksapatcharawong M.,Kasetsart University | Chanlekha H.,Kasetsart University | Khunthong V.,Kasetsart University | And 4 more authors.
Annual SRII Global Conference, SRII | Year: 2014

This work presents a system called Rice Watch, which has been developed to track, extract and process market price information and production yield for strategic planning in the economics and marketing of rice. Even though many sources of market information, such as commodity prices and stocks, are available on websites at any given time, such information is scattered and cannot be recomputed toward providing more usable information. Moreover, it is difficult to monitor large-scale areas under cultivation for production-market planning by using only surveys or statistical methods. Therefore, this work aims to apply integrated technologies, such as Information Extraction techniques, Machine aided Translation and Multi-Temporal Profile Matching techniques on satellite images, with two main goals: to provide market-information services to farmers and entrepreneurs for planning rice cultivation with better economic returns, and also to provide estimates for rice production at each stage of rice growth for market planning. © 2014 IEEE.


Lertmongkol S.,Kasetsart University | Sarobol E.,Kasetsart University | Premasthira C.-U.,Ministry of Agriculture and Cooperative
Kasetsart Journal - Natural Science | Year: 2011

Mungbean contains allelochemicals that can either inhibit or promote the growth and yield of subsequent crops in certain cropping systems. To examine the role of mungbean in a cropping system, the allelopathic effects of mungbean on the seed germination and plant growth of subsequent crops were evaluated in laboratory and pot experiments. In the laboratory experiment, the allelochemicals in mungbean inhibited the germination and root length of lettuce, whereas it had no negative effect on Echinochloa crus-galli seed germination. The pot experiment revealed that allelochemicals from decomposed mungbean in soil reduced the seed germination and plant height of subsequent crops especially in soybean (Glycine max) and lettuce (Lactuca sativa). The seed germination of soybean and lettuce was severely inhibited while the plant height of Echinochloa crus-galli was reduced. A high performance liquid chromatogram of the allelochemical compounds from the mungbean root and stem was composed of one major peak that had a retention time identical to that of thioglycerol and four other different peaks with one of these peaks having a retention time similar to that of aglycone.


Hotaka D.,Ministry of Agriculture and Cooperative | Amnuaykanjanasin A.,National Science and Technology Development Agency | Maketon C.,Kasetsart University | Siritutsoontorn S.,Kasetsart University | Maketon M.,Kasetsart University
Applied Entomology and Zoology | Year: 2015

Ten entomopathogenic fungi were tested for the control of female adult Thripspalmi Karny in the laboratory. The most virulent fungus Purpureocillium lilacinum CKPL-053, two commercial bioinsecticides and a chemical insecticide were tested for control of T. palmi in two Dendrobium sp. orchid farms. Controls of T. palmi in both farms were highest with P. lilacinum CKPL-053. Purpureocillium lilacinum CKPL-053 and the Odashima strain P. lilacinum produced paecilotoxins A, B, and C when cultured in complete medium 1 but not in potato dextrose broth. Jasmonic acid contents in Dendrobium sp. petals after application of P. lilacinum CKPL-053, B.bassiana CKB-048, M. robertsii CKM-048, and P. lilacinum CKPL-012 spore suspensions were significantly higher than with imidacloprid and water control treatments. This suggests that jasmonic acid might assist in activating plant defense mechanism to exhibit the repellence and/or resistance effect of Dendrobium sp. against T. palmi. The LC50 and LT50 for P. lilacinum CKPL-053 against female T. palmi were 2.67 × 105 conidia ml−1 and 2.6 days at the concentration of 1 × 108 conidia ml−1, respectively. © 2015, The Japanese Society of Applied Entomology and Zoology.


Khiaosa-ard R.,Chiang Mai University | Khiaosa-ard R.,ETH Zurich | Chungsiriwat P.,Ministry of Agriculture and Cooperative | Chommanart N.,Ministry of Agriculture and Cooperative | And 2 more authors.
Canadian Journal of Animal Science | Year: 2011

Belly, lean from the shoulder and backfat obtained from 80 pigs, fed either no or 1.6 kg tuna oil during fattening (35-90 kg body weight), were used to prepare bacon, Chinese-style sausage and Vienna-style sausage. The tuna oil had been supplemented either initially, at the end, or continuously during fattening. In all meat products, tuna oil supplementation clearly increased contents of n-3 fatty acids (FA), especially of the long-chain n-3 FA. Differences among supplementation periods were pronounced only in the n-3 FA proportion of total FA being lower with early tuna oil feeding. Thiobarbituric acid value, which was high in dry Chinese-style sausage, was mostly enhanced by tuna oil, whereas the period of tuna oil supplementation had no systematic influence. In conclusion, any mode of tuna oil supplementation investigated was efficient in enriching n-3 FA, but care should be taken in producing dry sausages due to their susceptibility to rancidity during storage time.


Singh K.,University of Prince Edward Island | Dey M.M.,University of Arkansas at Pine Bluff | Laowapong A.,Ministry of Agriculture and Cooperative | Bastola U.,Washington State University
Aquaculture Economics and Management | Year: 2015

We have examined the presence of price transmission asymmetry along the value chain, and the price transmission across four main aquaculture species in Thai fish market using monthly data from January 2001 to October 2010. This is an attempt to contribute to the literature on horizontal and vertical price transmission in the seafood markets including the price transmission asymmetry in the developing countries. We did not find any evidence of asymmetric price transmission in walking catfish (except in long-run), vannamei shrimp and tilapia; however, it is evident in Thai Asian sea bass market; wholesalers exercising some market power. In general, price of one species is not found to significantly affect price of the other species at the same level of value chain. © 2015, Taylor & Francis Group, LLC.


Uno K.,Aichi Konan College | Higashioto Y.,Yaizu Suisankagaku Industry Co. | Chaweepack T.,Chanthaburi Coastal Fisheries Research and Development Center | Ruangpan L.,Ministry of Agriculture and Cooperative
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2012

The present study examined the influences of industrial chitin extraction processes on the residual oxytetracycline (OTC) and oxolinic acid (OA) in shrimp carapaces and shells. The drugs were orally administered by catheter to the kuruma shrimp (Penaeus japonicus) and vannamei shrimp (Penaeus vannamei). The shrimps were sampled at 6-h post-dosing and their carapaces and shells were collected and used as raw material in the chitin extraction. Residual OTC levels in raw materials were 9 - 18 fold higher than the maximum residue limit (MRL) (0.2 μg/g). The residual OTC was reduced 10 - 30% by washing treatment. By the acid treatment with HCl, the OTC residues were reduced >98%. The deproteinized material contained negligible levels of OTC. Residual OA levels in raw materials was much higher (134 - 376 fold) than the MRL (0.03 μg/g). The residual OA was reduced considerably (34 - 68%) by washing treatment. By the demineralization, the residual OA could not be detected in both shrimp carapaces, while the levels of OA residues were determined more than MRL in shells. In the deproteinized materials, no residual OA were detected. Finally, residual OTC and OA could not be detected in the chitin materials for both shrimps. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey in cooperation with Japan International Cooperation Agency (JICA), Japan.


Uno K.,Aichi Konan College | Chaweepack T.,Chanthaburi Coastal Fisheries Research and Development Center | Ruangpan L.,Ministry of Agriculture and Cooperative
Aquaculture International | Year: 2010

The present study examined the pharmacokinetics and bioavailability of oxytetracycline (OTC) in vannamei shrimp (Penaeus vannamei) after intra-sinus (10 mg/kg) and oral (10 and 50 mg/kg) administration and also investigated the net changes of OTC residues in the shrimp after the thermal, acid and alkaline processing methods. The hemolymph concentrations of OTC after intra-sinus dosing were best described by a two-compartment open model. The oral bioavailability was found to be 48.2 and 43.6% at doses of 10 and 50 mg OTC/kg, respectively. The peak hemolymph concentrations after 10 and 50 mg OTC/kg doses were 3.37 and 17.4 μg/ml; the times to peak hemolymph concentrations were 7 and 10 h. The elimination half-lives were found to be 15. 0 and 11.5 h for the low and high dose, respectively. The residual OTC was rapidly eliminated from muscle with the elimination half-life value of 19.4 and 15.4 h, respectively, for the groups treated with doses of 10 and 50 mg/kg. The residual OTC levels in the muscle fell below the MRL (0.2 μg/g) at 72 and 96-h post-dosing at dose levels of 10 or 50 mg/kg, respectively. Residual OTC levels in muscle and shell were approximately 20-50% lower in the thermal treatment such as boiling, baking and frying. By the acid treatment, OTC residues were reduced to ≥80%, while those were reduced to around 30% by alkaline treatment. © 2010 Springer Science+Business Media B.V.


Moonjun R.,University of Twente | Udomsri S.,Ministry of Agriculture and Cooperative | Taiyarom A.,Development of Mineral Resources
33rd Asian Conference on Remote Sensing 2012, ACRS 2012 | Year: 2012

New approach of soil survey methods is known as "digital soil mapping(DSM)", requires new techniques such as geo-information system and remote sensing techniques to produce accurate soil map. Airborne gamma-ray imagery (AGRI) is taken into an account for the very important source of information for soil study at regional scale. The ratio of eTh and K concentrations as representing soil component and chemical concentration during the rock weathering process. Digital terrain modelling (DTM) is representing topographic feature, explaining the landform pattern as the most important soil forming factor. Aim of this study was to combine ratio of gamma-ray and CTI as a covariable using statistical modeling as local predicting approach. Geographically Weight Regression (GWR) was tested to predict percent of top-soil clay CTI, using GWR method can therefore be possible to map of top-soil pH and percent clay.

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