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Navi Mumbai, India

Deshpande R.H.,MIMSR Medical college Latur | Kulkarni R.V.,MIMSR Medical college Latur
International Journal of Pharma and Bio Sciences

Aim of the case report was to present a rare case of CAD with Malaria. Methods and materials were as per the clinical protocol and investigations done in the department of pathology.Cold Agglutinin Disease (CAD) is a subgroup of autoimmune Haemolytic Anemia (AIHA). Cold reactive immune globulins (agglutinins) which are directed against erythrocyte surface antigen ('1' antigen) are essential for the pathogenesis of CAD. Classically, the patients with CAD present with chronic anemia and acrocyanosis. Several factors like the antibody titre and the temperature range determine the ability of Cold agglutinin to induce haemolysis. The conclusion of study was that,the specific problem that occurs in the laboratory due to pathologic cold agglutinins need to be kept in mind for the accurate diagnosis. Here, we are reporting a case with of CAD in association with malaria in a 13 yrs old female. Source

Shital P.,MIMSR Medical college Latur | Rujuta A.,MIMSR Medical college Latur | Sanjay M.,MIMSR Medical college Latur
Journal of Cancer Research and Clinical Oncology

Background: Lung cancer is the leading cause of cancer deaths around the world. Globally, lung cancer is the largest contributor to new cancer diagnosis and to death from cancer. Various conventional diagnostic techniques (CDTs) such as endobronchial forcep biopsy (FB), bronchial washing (BW) and bronchial brushing (BB), and transbronchial needle aspiration cytology (TBNA) are employed during fiber-optic bronchoscopy. Methods: This is a prospective study conducted between June 2012 and September 2013 at Bronchoscopy unit of MIMSR Medical College Latur, India, to find the role of TBNA in exophytic endobronchial lesions in confirming the diagnosis of lung cancer and to find additive yield over other techniques such as BB, BW and FB, and included 150 patients on the basis of clinical and radiological features of malignancy. In exophytic endobronchial lesions, predominant endoscopic findings were cauliflower, polypoidal-like or nodular or multinodular endobronchial growth. TBNA, FB, BB and BW were performed in all the cases during FOB. Histopathological and cytological examinations of specimens were performed at Pathology department. The statistical analysis was done using chi-square test. Results: Total 150 patients, between age group 24-80 years, mean age was 57.93 years, male population constitutes 86.17 % of total. 76.14 % cases were smoker of them 61.87 % cases having history of >40 pack years. Commoner symptoms were cough (91.33 %), hemoptysis (54.00 %) and chest pain (46.66 %), and mass lesion (40.66 %), hilar opacity (31.33 %) and collapse segmental/lobar (29.33 %) were commoner radiological abnormalities. Yield of TBNA, CDT and TBNA plus CDT in exophytic lesions is 60.66, 79.33 and 84.66 %, respectively, in diagnosis of lung malignancies (P < 0.001). Sensitivity of forcep biopsy and TBNA in diagnosing lung malignancy was 88.18 and 71.65 %, respectively (P < 0.01). FOB-related hypoxemia documented in two cases and minor bleeding in six cases. Other complications such as significant bleeding, pneumothorax and death were not seen. Minor bleeding was seen with forcep biopsy mainly in 4.0 % cases. TBNA has decreased repeat procedure, decreases cost for diagnosis. IHC on TBNA specimens had increased histological-type confirmation. Conclusion: Transbronchial needle aspiration is a beneficial, safe and minimally invasive bronchoscopic technique with insignificant side effect in the diagnosis bronchogenic carcinoma. TBNA considered safe, especially when fleshy vascular endobronchial growth is present and risk of bleeding is high with forcep biopsy. Inadequate tissue sampling due to the presence of necrosis, blood clot over the lesion and formation of crush artifacts by FB makes TBNA valuable in these lesions. TBNA will definitely decrease need for repeat bronchoscopy. © 2014 Springer-Verlag. Source

Nagoba B.S.,MIMSR Medical college Latur | Kasture R.M.,MIMSR Medical college Latur
Research Journal of Pharmaceutical, Biological and Chemical Sciences

Improved bioavailability is an added advantage for most of the poorly soluble drugs in water. In recent years research work is concentrated on various methods to improve the solubility characteristics of poorly soluble drugs and crystallization phenomenon is one among them. The solubility problem can be solved by changing the crystal habit of drug, which improves the solubility and dissolution. Crystallization is also a purification process to remove the impurities from pharmaceutical products by, recrystallization technique. So, in the present investigation an attempt has been made to improve the solubility characteristics of Meloxicam (NSAID's) using crystallization method by solvent evaporation technique. To increase the therapeutic efficiency and quality of existing marketed dosage formulations. In this method, crystallization takes places mainly due to the removal of solvent by evaporation and reprecipitation in water in which drug is insoluble. The biphasic layer formed due to water immiscible solvent and is evaporated by maintaining the temperature above to corresponding boiling point of solvents. In our work two immiscible solvents, chloroform and ethyl acetate used and microcrystals prepared under slow and turbulent stirring conditions. The precipitated crystals were filtered using whatmann paper and dried at 60° C for 1 hour. The formulated crystals of Meloxicam were subjected to various physico-chemical parameters like size distribution, shape and drug, solvent interactions with DSC, IR, XRD etc., and found to be smaller in size than pure and crystalline in nature with free from any interactions. For all the samples in-vitro drug release parameters studied U.S.P. XXIII dissolution rate test apparatus (Electrolab) employing paddle stirrer for one hour. In 900 ml phosphate buffer (pH 7.4) and compared with pure drug samples. The microcrystals produced with 30% v/v chloroform and 30% v/v ethyl acetate as solvents prepared under turbulent stirring conditions precipitated in water as crystallization media found to be best formulations for improved drug dissolution when compared to the pure drug for Meloxicam respectively. The results thus conclusively proved that the method of precipitation by solvent evaporation technique can be used to produce microcrystals of poorly soluble non-steroidal anti-inflammatory drugs. Source

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