Miltenyi Biotec GmbH

Germany

Miltenyi Biotec GmbH

Germany
SEARCH FILTERS
Time filter
Source Type

Patent
Miltenyi Biotec GmbH | Date: 2017-04-13

The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 13545 to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. Furthermore, the invention relates to a method for modifying cells comprising the steps


Patent
Miltenyi Biotec GmbH | Date: 2017-06-28

The present invention provides chimeric antigen receptors (CARs) comprising an antigen binding domain specific for SSEA4, a population of engineered cells expressing said CARs, and a pharmaceutical composition comprising said genetically modified cells expressing said CARs. The pharmaceutical composition may be for use of the treatment of cancer in a subject suffering from cancer, wherein at least a subpopulation of the cancerous cells of said cancer expresses SSEA4.


The present invention provides a chimeric antigen receptor (CAR), comprising an extracellular part, at least one intracellular signaling domain, and at least one transmembrane domain, wherein the extracellular part of said CAR comprises a) at least one antigen binding domain, and b) at least one cytokine receptor activating or blocking domain. The invention also provides isolated nucleic acid molecule(s) encoding for the said CAR, a cell comprising said nucleic acid molecule(s), a cell expressing said CAR and therapeutic uses of said CAR.


Patent
Miltenyi Biotec GmbH | Date: 2017-03-08

The present invention provides a method for in-vitro culturing and expanding natural killer (NK) cells in a cell culture medium comprising a population of NK cells, the method comprising a) adding an effective concentration of interleukin-21 (IL-21) at the beginning of the culturing process to said medium, b) adding repeatedly an effective concentration of interleukin-2 (IL-2) and/or interleukin-15 (IL-15) to said medium, and c) adding repeatedly feeder cells or membrane particles thereof to said medium, wherein said feeder cells are B cell derived which are EBV immortalized; and wherein said expansion of NK cells in said cell culture medium is maintained for at least 3 weeks.


Patent
Miltenyi Biotec GmbH | Date: 2017-03-08

The invention is directed to disposable for electroporation of cells, comprisinga fluid compartment in an interior of the disposable;a first fluid port for providing cell suspension to the fluid compartment, and a second fluid port for delivering a fluid comprising at least one compound to be electroporated into the cells to the fluid compartment;a first electrode and a second electrode disposed in the fluid compartment;at least one exit port which delivers the fluid from the fluid compartment wherein the first and second fluid port have a fluid communication to a mixing channel which has a fluid communication to the fluid compartment.


Patent
Miltenyi Biotec GmbH | Date: 2017-05-24

The invention is directed to a Perfusion device for biological tissues comprising- a casing having two parts, a first part (1) and a second part (9),- a holder (7) for a plurality of hollow penetration structures (8), wherein the hollow penetration structures (8) are provided with at least one orifice having fluid communication through the holder (7)- a support (5) for the biological tissue (6)characterized in that the support (5) for the biological tissue (6) is positioned in the casing at a distance to the holder (7) that by joining the first part (1) and the second part (9) to form the casing, the hollow penetration structures (8) are in proximity to the holder (7). Use of the perfusion device in a process for disaggregation of a biological tissue to yield target cells.


Patent
Miltenyi Biotec GmbH | Date: 2017-01-11

The invention is directed to a process for sorting target cells and non-target cells from a sample by a cell sorting valve microfabricated on a surface of a silicon substrate, with microfabricated channels leading from the cell sorting valve, wherein the cell sorting valve separates the target particles from non-target material;a disposable cartridge containing a sample reservoir, a sort reservoir and a waste reservoir; wherein the sample is provided in a buffer comprising nuclease.


Patent
Miltenyi Biotec GmbH | Date: 2017-01-11

A MEMS-based cell sorter is disclosed, which uses a novel combination of features to accomplish the cell sorting using a microfabricated cell sorting valve housed in a disposable cartridge. The features include an interposer that provides fluid communication between the microfluidic passages in the silicon substrate and a plurality of fluid reservoirs in the cartridge, including a sample, sort and waste reservoir The disposable cartridge may include other features that assist in the handling of small volumes of fluids, such as a siphon region in the sort reservoir and funnel-shaped regions in the sample and waste reservoirs. A mixing mechanism may be provided for stirring the contents of the sample reservoir.


Patent
Miltenyi Biotec GmbH | Date: 2017-03-01

The present invention provides a process for generation of genetically modified T cells, T cell subsets and/or T cell progenitors comprising the steps: a) providing a cell sample comprising T cells, T cell subsets and/or T cell progenitors b) preparation of the cell sample by centrifugation c) magnetic separation of the T cells, T cell subsets and/or T cell progenitors d) activation of the enriched T cells, T cell subsets and/or T cell progenitors using modulatory agents e) genetic modification of the T cells, T cell subsets and/or T cell progenitors f) expansion of the genetically modified T cells, T cell subsets and/or T cell progenitors in a cultivation chamber g) washing of the cultured T cells, T cell subsets and/or T cell progenitors characterized in that all steps are performed in a closed and sterile cell culture system.


Patent
Miltenyi Biotec GmbH | Date: 2017-01-11

A MEMS-based cell sorting systenn is disclosed, which uses a novel combination of features to accomplish the cell sorting in the microfabricated channels housed in a disposable cartridge. The MEMS-based cell sorting system may include a microfabricated cell sorting valve that is responsive to an applied magnetic field. The MEMS-based cell sorting system may further include an electromagnet that generates a magnetic field to actuate the microfabricated cell sorting valve. The electromagnet may have a design which allows it to create a very localized magnetic field while having adequate thermal properties to operate reliably.

Loading Miltenyi Biotec GmbH collaborators
Loading Miltenyi Biotec GmbH collaborators