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Prague, Czech Republic

Kovandova H.,Czech Technical University | Valka R.,Military University Hospital Prague
Promet - Traffic - Traffico | Year: 2014

The paper deals with safety of transport from the point of view of an important social problem, which are accidents at railroad (including tramway) and road level crossings, which means compatibility of rail and road vehicles crash. The scale of such accidents can be very wide. Regarding the statistics and frequency of accidents particularly at railway level crossings and tramway level crossings, a collision simulating the collision of a tram and passenger car was experimentally carried out. The experiment took place at the site of testing laboratory of Rail Vehicles Research Institute in Cerhenice. The experiment was conducted with a passenger car Škoda Superb of the first generation that was exposed to two collisions from both sides. Firstly by the impactor for tram headstock tests and secondly by the tram headstock itself. Both the impactor and the headstock were placed on the experimental vehicle for tests of passive safety of rail vehicles. Various speeds were chosen so that the passenger car could be used for two subsequent experiments without the influence on properties of skeleton´s supporting structure. © 2014, Faculty of Transport and Traffic Engineering. All rights reserved.

Nemec I.,Military University Hospital Prague
Acta Chirurgiae Plasticae | Year: 2013

Closure of a nasal tip defect has specific characteristics with regards to the shape, colour, thickness and texture of skin. During the reconstruction it is necessary to observe aesthetic and functional characteristics to achieve an optimal result. There are several methods for closure of a defect in this area. One of the possible techniques is usage of a local flap. In the selection of an optimal procedure it is suitable to consider the aesthetic subunits in the nose. Usage of the V-Y flap is beneficial compared to a rotation flap or a transposition flap, which are associated with significant scaring and deformities, such as a dog-ear. Such conditions may require further corrections later. Ercocen et al. described a V-Y island dorsal nasal flap, which they used to close a nasal tip defect. The blood supply of the flap was based on the terminal branches of angular artery on both sides (1). In our case report we used the aforementioned flap to close a nasal defect. The flap was based on the terminal branches of the angular artery only on one side.

Vitku J.,Institute of Endocrinology | Chlupacova T.,Institute of Endocrinology | Sosvorova L.,Institute of Endocrinology | Hampl R.,Institute of Endocrinology | And 5 more authors.
Talanta | Year: 2015

Abstract Bisphenol A (BPA) is a widely known endocrine disruptor with estrogenic, antiestrogenic or antiandrogenic properties. BPA could interfere with estrogen metabolism as well with receptor-mediated estrogen actions. Both environmental BPA and estrogens may be traced in body fluids, of which, besides the blood plasma, the seminal fluid is of particular interest regarding their possible interactions in the testis. The method for simultaneously determining BPA and estrogens is then needed, taking into account that their concentrations in these body fluid may differ. Here the method was developed and validated for measurements of BPA, estrone (E1), estradiol (E2) and estriol (E3) in blood plasma and seminal plasma using liquid chromatography-tandem mass spectrometry. Due to the phenolic moiety of all compounds, dansyl chloride derivatization could be used. The analytical criteria of the method with respect to expected concentration of the analytes were satisfactory. The lower limits of quantifications (LLOQ) amounted to 43.5, 4.0, 12.7, 6.7 pg/mL for plasma BPA, E1, E2 and E3, and 28.9, 4.9, 4.5, 3.4 pg/mL for seminal BPA, E1, E2 and E3, respectively. The concentrations of individual steroids differed between body fluids. To the best of our knowledge, this is the first method that enabled the measurement of estrogens and BPA together in one run. The concentrations of E1, E2 and for the first time also of E3 in seminal plasma in normospermic men are reported. © 2015 Elsevier B.V. All rights reserved.

Vitku J.,Institute of Endocrinology | Sosvorova L.,Institute of Endocrinology | Chlupacova T.,Institute of Endocrinology | Hampl R.,Institute of Endocrinology | And 6 more authors.
Physiological Research | Year: 2015

The general population is potentially exposed to many chemicals that can affect the endocrine system. These substances are called endocrine disruptors (EDs), and among them bisphenol A (BPA) is one of the most widely used and well studied. Nonetheless, there are still no data on simultaneous measurements of various EDs along with steroids directly in the seminal fluid, where deleterious effects of EDs on spermatogenesis and steroidogenesis are assumed. We determined levels of BPA and 3 estrogens using LC-MS/MS in the plasma and seminal plasma of 174 men with different degrees of infertility. These men were divided according their spermiogram values into 4 groups: (1) healthy men, and (2) slightly, (3) moderate, and (4) severely infertile men. Estradiol levels differed across the groups and body fluids. Slightly infertile men have significantly higher BPA plasma and seminal plasma levels in comparison with healthy men (p<0.05 and p<0.01, respectively). Furthermore, seminal BPA, but not plasma BPA, was negatively associated with sperm concentration and total sperm count (-0.27; p<0.001 and -0.24; p<0.01, respectively). These findings point to the importance of seminal plasma in BPA research. Overall, a disruption of estrogen metabolism was observed together with a weak but significant impact of BPA on sperm count and concentration. © 2015 Institute of Physiology v.v.i.

Sosvorova L.,Institute of Endocrinology | Mohapl M.,Military University Hospital Prague | Vcelak J.,Institute of Endocrinology | Hill M.,Institute of Endocrinology | And 2 more authors.
Physiological Research | Year: 2015

Cytokines are widely known mediators of inflammation accompanying many neurodegenerative disorders including normal pressure hydrocephalus (NPH). NPH is caused by impaired cerebrospinal fluid (CSF) reabsorption and treated by surgical shunt insertion. The diagnostics is still complicated and the shunt effect is not durable; after several years, dementia may develop. In the clinical practice, biomarkers support the diagnostics as well as the further time course of many neurodegenerative diseases. Until recently, no reliable biomarker for NPH was evaluated. The attempt of this review was to make a survey concerning cytokines as possible NPH markers. Among all reviewed cytokines, the most promising are CSF IL-10 and IL-33, enabling to follow-up the disease progression and monitoring the effectiveness of the shunt insertion. © 2015 Institute of Physiology v.v.i.

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