Military Technical Institute of Protection

Brno, Czech Republic

Military Technical Institute of Protection

Brno, Czech Republic
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Gryc K.,VSB - Technical University of Ostrava | Stransky K.,Brno University of Technology | Michalek K.,VSB - Technical University of Ostrava | Winkler Z.,Military Technical Institute of Protection | And 6 more authors.
Materiali in Tehnologije | Year: 2012

The paper is devoted to selected aspects of the current issues of the high-temperature interaction of the synthetic multicomponent oxidic systems and selected grades of steel. Analysing the consequences of the interaction between slag and molten steel is an integral part of modern research that can be connected with subsequent, applied research in the field of producing and refining steel. The interactions between the synthetic slag and the molten metal is realized after the processing of the liquid steel in the primary metallurgical aggregates, i.e., at the beginning of refining processes of secondary metallurgy. The addition of these synthetic systems (slag) significantly affects many technological processes and indirectly affects the final quality of the cast steel. This influence can be seen on two levels: the metallurgical and the metallographic. Both of these levels of evaluation complement each other. For the evaluation of the metallurgical aspects of refining processes a number of thermodynamic relations were used, supplemented by empirically established formulas and coefficients. Metallographic analyses utilize modern tools in the study of the structure and the chemical composition of materials, i.e., from light microscopy to sophisticated systems of microanalysis of elemental composition using a scanning electron microscope combined with an energy-dispersive X-ray micro-analyser. It is obvious that this area deserves more focused research and more attention, particularly in the context of the on-going needs for the identification and quantification of phenomena taking place in the following metallurgical innovations.

Skladal P.,Masaryk University | Skladal P.,Military Technical Institute of Protection | Svabenska E.,Masaryk University | Svabenska E.,Military Technical Institute of Protection | And 5 more authors.
Electroanalysis | Year: 2012

The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5α in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108CFU/mL within 30min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150CFU/L in air was clearly indicated (20min analysis including 5min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Szklorzova H.,Research Institute of Building Materials | Holesinsky R.,Research Institute of Building Materials | Kratky J.,Military Technical Institute of Protection | Krestan J.,Military Technical Institute of Protection | And 2 more authors.
American Concrete Institute, ACI Special Publication | Year: 2012

The experimental work is aimed at designing the concrete mixtures for special applications, in which several types of aggregate and reinforcement are used. The effects of aggregate types and sizes and types of reinforcement (oriented and randomly dispersed) are presented. The reinforcements used are various metallic and non-metallic nets and dispersed fibers. Mechanical and explosion tests were performed and the effect of different kinds of aggregate and reinforcement on explosion resistance and mechanical performance of the concrete samples were evaluated.

Halamek E.,University of Defense | Kadlcak J.,Military Technical Institute of Protection
Defence Science Journal | Year: 2012

To detect nerve agents in practice, the analytical methods such as gas, liquid and thin-layer chromatography, mass spectrometry or capillary electrophoresis are usually used. Apart from these analytical methods, we developed an analytical device (tape photocolorimetric biosensor) based on the modified Ellmaǹs cholinesterase biochemical reaction for multidetection of cholinergic organophosphorus compounds. Enzyme butyrylcholinesterase was used as a biorecognizing component and its activity was evaluated by red, blue, green (RGB) sensor. This method eliminates errors in the evaluation and provides automatic data collection with their subsequent evaluation. The unique method of dosing allows appropriate dispensing of reagents in microlitres volumes and the whole system is simple to operate. Suitability of the constructed biosensors was evaluated using the six organophosphates (Tabun, sarin, Soman, cyclosin, VX and R33 compound). Biosensor showed the ability to measure substances at concentrations ranging between ~ 1×10-8 mg/l - 1×10 -6 mg/l in the air, according to their inhibition effect. © 2012, DESIDOC.

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