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Kim S.,Konkuk University | Lee S.Y.,Konkuk University | Han J.-K.,MILAE Resources ML Co. | Lee J.-K.,Kyonggi University | Choi M.-J.,Konkuk University
Korean Journal of Food Science and Technology | Year: 2015

Lactobacillus is a probiotic that suppresses the growth of pathogens while preventing constipation, diarrhea, and intestinal inflammation. However, various environmental conditions such as pH and temperature affect the growth of Lactobacillus. In this study, Lactobacillus plantarum was encapsulated with starch using a spray dryer to protect the viability of the organism during storage and to increase its acid tolerance. The lower water activity and storage temperature resulting from this method influenced the survival of L. plantarum. In encapsulated Lactobacillus powders, viability of Lactobacillus was increased during storage at 20°C relative to that of L. plantarum stored at 35°C in the same water activity conditions. Furthermore, L. plantarum encapsulated in starch with ginger showed increased viability when compared to non-encapsulated L. plantarum stored without treatment. Finally, based on a simulated digestion test, encapsulated L. plantarum survived at a pH of 2, whereas the non-encapsulated L. plantarum did not. Thus, coating the encapsulated powder with these materials was effective in maintaining Lactobacillus viability.

Sim I.,Korea University | Park K.-T.,Korea University | Park K.-T.,MILAE Resources ML Co. | Lim Y.-H.,Korea University
Microbiology and Biotechnology Letters | Year: 2016

The aim of this study was to investigate the potential of lactic acid bacteria (LAB) strains as probiotics. Two strains were isolated from healthy chicken cecum and their acid and bile tolerance, residual organic acids, antibacterial activity against pathogenic bacteria, and immunomodulation activity were measured. Identification of the isolated strains was performed using the API 50CHL system and phylogenetic analysis using 16S rDNA sequencing. The isolates were determined to be Lactobacillus sakei strains. The acid tolerance of strains L2 and L8 was high enough that 75% of the inoculum survived in pH 2 for 2 h. The bile tolerance of both strains was observed at a 1% Oxgall concentration in MRS broth. The production of organic acids (lactic acid and acetic acid) and pH changes during growth were monitored and the maximum concentrations were obtained after 48 h of incubation. Culture supernatants of the two LAB strains showed strong antibacterial activity against pathogenic bacteria. The heat-killed LAB cells also induced high levels of immune cell proliferation compared with the control, and stimulated IL-6 and TNF-α production in mouse macrophages. Therefore, L. sakei strains L2 and L8 can be considered suitable probiotic bacteria. © 2016, The Korean Society for Microbiology and Biotechnology.

Lee S.-Y.,Konkuk University | Jo Y.-J.,Konkuk University | Choi M.-J.,Konkuk University | Lee B.-Y.,Korea University | And 3 more authors.
Korean Journal for Food Science of Animal Resources | Year: 2014

This study was designed to find the most suitable method and wall material for microencapsulation of the Lactobacillus plantarum to maintain cell viability in different environmental conditions. To improve the stability of L. plantarum, we developed an encapsulation system of L. plantarum, using water-in-oil emulsion system. For the encapsulation of L. plantarum, corn starch and glyceryl monostearate were selected to form gel beads. Then 10% (w/v) of starch was gelatinized by autoclaving to transit gel state, and cooled down at 60°C and mixed with L. plantarum to encapsulate it. The encapsulated L. plantarum was tested for the tolerance of acidic conditions at different temperatures to investigate the encapsulation ability. The study indicated that the survival rate of the microencapsulated cells in starch matrix was significantly higher than that of free cells in low pH conditions with relatively higher temperature. The results showed that corn starch as a wall material and glycerol monostearate as a gelling agent in encapsulation could play a role in the viability of lactic acid bacteria in extreme conditions. Using the current study, it would be possible to formulate a new water-in-oil system as applied in the protection of L. plantarum from the gastric conditions for the encapsulation system used in chicken feed industry.

Chon H.,MILAE Resources ML Co. | Choi B.,MILAE Resources ML Co. | Jeong G.,Seoul National University | Lee E.,Korea Research Institute of Bioscience and Biotechnology | Lee S.,Korea Research Institute of Bioscience and Biotechnology
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

The objective of this study was to evaluate the immunomodulatory effects of specific bacterial metabolites of Lactobacillus plantarum 10hk2 to induce anti-inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264.7. The effects of the extracellular metabolites of this bacterial strain were examined by dividing them into protein and polysaccharide fractions. A specific protein fraction (8.7. kDa) was found to be a strong IL-10 inducer in LPS-stimulated RAW 264.7 cells and suppressed LPS-induced NF-κB induction and inhibited LPS-induced phosphorylation of I-κB and p38 MAPK. To the best of our knowledge, this was the first study that investigated the anti-inflammatory effects of an extracellular peptide derived from lactic acid bacteria. In addition, we characterized the inhibitory mode of this molecule in the induction of proinflammatory cytokines. Based on the findings presented in this study, this molecule holds promise for use as an agent to modulate inflammation related diseases. © 2009 Elsevier Ltd.

Chon H.,MILAE Resources ML Co. | Kim G.,MILAE Resources ML Co. | Kim S.,MILAE Resources ML Co.
Natural Product Communications | Year: 2010

This study was conducted to investigate the antioxidant properties and inhibitory effects on the inflammation-related cytokines of plant extracts fermented with Lactobacillus paracasei LS-2 in comparison with the unfermented aqueous plant extract. Attempted have also been made to identify fermented plant extracts that display no cytotoxicity against murine macrophage cells, while still maintaining their biological characteristics. Most of the fermented plant extracts showed reduced cytotoxicity. Among the fourteen plant extracts tested, the fermented plant extract of Scutellaria baicalensis Georgi increased the induction of anti-inflammatory mediators; however, they were still cytotoxic. Interestingly, the plant extract of Artemisia capillaris Thunb. induced a dramatic increase in the induction of anti-inflammatory mediators with no cytotoxicity through fermentation. Our findings suggest that fermented A. capillaris holds promise for use as a valuable natural non-cytotoxic antioxidant and immuno-modulating agent.

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