MILAE Resources ML Co.

South Korea

MILAE Resources ML Co.

South Korea
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Park S.,Kangwon National University | Kim C.Y.,Kangwon National University | Park B.,Kangwon National University | Kim K.,Kangwon National University | And 3 more authors.
Korean Journal of Veterinary Research | Year: 2016

The Ecklonia cava Kjellman by-product (ECBP) as a feed additive was evaluated in improvement of productivity and immune enhancement against Salmonella Gallinarum (SG). Lohmann Brown chickens proved SGfree were randomly divided into 3 groups of 8 chickens each. Chickens were fed with the experimental diet treatment: T0, Non treatment-commercial feed; T1, commercial feed with 0.5% ECBP; T2, commercial feed with 0.1% Lactobacillus plantarum. In this study, we evaluated the effect of T1 and T2 groups on the body weight and protective efficacy against SG in chickens. The results demonstrated that treatment of T1 group as a feed additive affected significantly body weight gaining in chickens. In addition, T1 group showed a significant different colonization of SG when compared to T2 and T0 groups. We also studied that serum IgG and interferon-ã levels were significantly different compared with other treatment groups. Therefore, we suggest that ECBP can be used as a good candidate of feed additives in chicken industry.


Ohh M.H.,Korea University | Kim S.J.,Milae Resources ML. Co. | Han J.K.,Milae Resources ML. Co. | Pak S.C.,Charles Sturt University | Chee K.-M.,Korea University
Journal of Intercultural Ethnopharmacology | Year: 2016

Aim: Uterine leiomyomas are the most common benign uterine neoplasms associated with significant morbidity. Herbal formulas capable of restoring yin-yang balance by dispersing blood stasis may be useful for managing fibroid symptoms. Materials and Methods: In this study, the antitumor properties of three herbs viz., Trogopterus xanthipes Milen-Edwards, Paeonia lactiflora Pallas, and Ulmus davidiana Planch were evaluated in nude mice injected intravenously with human malignant myomas. Tumor fragments were xenografted subcutaneously through a flank incision in female mice. The mice entered the study for 8 weeks when their tumors reached the threshold volume (260 mm3). The mice were randomly allocated to receive subcutaneous injections of normal saline (Group 1; negative control), P. lactiflora Pallas (Group 2), U. davidiana Planch (Group 3), T. xanthipes Milen-Edwards (Group 4), and intravenous injections of paclitaxel (Group 5; positive control). The weight and tumor volume were measured, followed by histopathology. Results: A few cases of abdominal distention and death were observed in the negative control group. Furthermore, a considerable enlargement of the liver and spleen was observed in the negative control group at autopsy with a gradual increase in body weight during the experiment. The mean tumor volume which increased in negative control mice reduced in mice treated with herbal remedies or paclitaxel from day 14 onwards (P < 0.05). The degree of necrosis and apoptosis induction from herbal treatments was similar to that of paclitaxel. Conclusion: Collectively, three herbs viz., T. xanthipes Milen-Edwards, P. lactiflora Pallas, and U. davidiana Planch were able to induce necrosis and apoptosis of uterine leiomyoma cells, proving antitumor properties against uterine fibroids. © SAGEYA.


Ohh M.-H.,Korea University | Kim S.,MILAE RESOURCES ML. Co. | Pak S.C.,Charles Sturt University | Chee K.-M.,Korea University
Asian-Australasian Journal of Animal Sciences | Year: 2016

Astaxanthin (ASX) is a xanthophyll pigment isolated from crustaceans and salmonids. Owing to its powerful antioxidant activity, ASX has been reported to have the potential to protect against gastric ulcers and a variety of other illnesses. Histamine (His) is a dietary factor that causes gastric erosion and ulceration in young chicks. In this study, we examined whether ASX had protective effects on dietary histamine-induced lesions in the gizzard and proventriculus of broiler chickens. Four experimental treatment groups were planned: basal diet (BD), BD+His, BD+ASX, and BD+ASX+His, with four chicks (5 days old) in each group and three replications (i.e., a total of 12 chicks per group). The BD was supplemented with either 0.4% His or 100 ppm ASX. The birds were fed ad libitum for 3 weeks, and diets contained no antimicrobial compounds. Supplementing the diet with His significantly decreased body weight gain, but increased the weights of the gizzard and proventriculus of the chicks as compared with those of chicks in the BD group (p<0.05). ASX did not affect His-dependent changes in chick body weight or weights of the gizzard and proventriculus. The loss of gastric glands in the proventriculus, which was observed in His-treated chicks, was not prevented by ASX administration. The frequency of proventricular ulceration, however, was lowered by treatment with ASX, without significant differences between the two supplementation levels. In conclusion, our data showed that ASX might be helpful for alleviating structural damage to the digestive system in poultry under certain stressful conditions. Copyright © 2016 by Asian-Australasian Journal of Animal Sciences.


Chon H.,Milae Resources ML Co. | Choi B.,Milae Resources ML Co. | Jeong G.,Seoul National University | Lee E.,Korea Research Institute of Bioscience and Biotechnology | Lee S.,Korea Research Institute of Bioscience and Biotechnology
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

The objective of this study was to evaluate the immunomodulatory effects of specific bacterial metabolites of Lactobacillus plantarum 10hk2 to induce anti-inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264.7. The effects of the extracellular metabolites of this bacterial strain were examined by dividing them into protein and polysaccharide fractions. A specific protein fraction (8.7. kDa) was found to be a strong IL-10 inducer in LPS-stimulated RAW 264.7 cells and suppressed LPS-induced NF-κB induction and inhibited LPS-induced phosphorylation of I-κB and p38 MAPK. To the best of our knowledge, this was the first study that investigated the anti-inflammatory effects of an extracellular peptide derived from lactic acid bacteria. In addition, we characterized the inhibitory mode of this molecule in the induction of proinflammatory cytokines. Based on the findings presented in this study, this molecule holds promise for use as an agent to modulate inflammation related diseases. © 2009 Elsevier Ltd.


Sim I.,Korea University | Park K.-T.,Korea University | Park K.-T.,Milae Resources ML Co. | Lim Y.-H.,Korea University
Microbiology and Biotechnology Letters | Year: 2016

The aim of this study was to investigate the potential of lactic acid bacteria (LAB) strains as probiotics. Two strains were isolated from healthy chicken cecum and their acid and bile tolerance, residual organic acids, antibacterial activity against pathogenic bacteria, and immunomodulation activity were measured. Identification of the isolated strains was performed using the API 50CHL system and phylogenetic analysis using 16S rDNA sequencing. The isolates were determined to be Lactobacillus sakei strains. The acid tolerance of strains L2 and L8 was high enough that 75% of the inoculum survived in pH 2 for 2 h. The bile tolerance of both strains was observed at a 1% Oxgall concentration in MRS broth. The production of organic acids (lactic acid and acetic acid) and pH changes during growth were monitored and the maximum concentrations were obtained after 48 h of incubation. Culture supernatants of the two LAB strains showed strong antibacterial activity against pathogenic bacteria. The heat-killed LAB cells also induced high levels of immune cell proliferation compared with the control, and stimulated IL-6 and TNF-α production in mouse macrophages. Therefore, L. sakei strains L2 and L8 can be considered suitable probiotic bacteria. © 2016, The Korean Society for Microbiology and Biotechnology.


Lee S.-Y.,Konkuk University | Jo Y.-J.,Konkuk University | Choi M.-J.,Konkuk University | Lee B.-Y.,Korea University | And 3 more authors.
Korean Journal for Food Science of Animal Resources | Year: 2014

This study was designed to find the most suitable method and wall material for microencapsulation of the Lactobacillus plantarum to maintain cell viability in different environmental conditions. To improve the stability of L. plantarum, we developed an encapsulation system of L. plantarum, using water-in-oil emulsion system. For the encapsulation of L. plantarum, corn starch and glyceryl monostearate were selected to form gel beads. Then 10% (w/v) of starch was gelatinized by autoclaving to transit gel state, and cooled down at 60°C and mixed with L. plantarum to encapsulate it. The encapsulated L. plantarum was tested for the tolerance of acidic conditions at different temperatures to investigate the encapsulation ability. The study indicated that the survival rate of the microencapsulated cells in starch matrix was significantly higher than that of free cells in low pH conditions with relatively higher temperature. The results showed that corn starch as a wall material and glycerol monostearate as a gelling agent in encapsulation could play a role in the viability of lactic acid bacteria in extreme conditions. Using the current study, it would be possible to formulate a new water-in-oil system as applied in the protection of L. plantarum from the gastric conditions for the encapsulation system used in chicken feed industry.


Kim S.,Konkuk University | Lee S.Y.,Konkuk University | Han J.-K.,MILAE Resources ML Co. | Lee J.-K.,Kyonggi University | Choi M.-J.,Konkuk University
Korean Journal of Food Science and Technology | Year: 2015

Lactobacillus is a probiotic that suppresses the growth of pathogens while preventing constipation, diarrhea, and intestinal inflammation. However, various environmental conditions such as pH and temperature affect the growth of Lactobacillus. In this study, Lactobacillus plantarum was encapsulated with starch using a spray dryer to protect the viability of the organism during storage and to increase its acid tolerance. The lower water activity and storage temperature resulting from this method influenced the survival of L. plantarum. In encapsulated Lactobacillus powders, viability of Lactobacillus was increased during storage at 20°C relative to that of L. plantarum stored at 35°C in the same water activity conditions. Furthermore, L. plantarum encapsulated in starch with ginger showed increased viability when compared to non-encapsulated L. plantarum stored without treatment. Finally, based on a simulated digestion test, encapsulated L. plantarum survived at a pH of 2, whereas the non-encapsulated L. plantarum did not. Thus, coating the encapsulated powder with these materials was effective in maintaining Lactobacillus viability.


Chon H.,Milae Resources ML Co. | Kim G.,Milae Resources ML Co. | Kim S.,Milae Resources ML Co.
Natural Product Communications | Year: 2010

This study was conducted to investigate the antioxidant properties and inhibitory effects on the inflammation-related cytokines of plant extracts fermented with Lactobacillus paracasei LS-2 in comparison with the unfermented aqueous plant extract. Attempted have also been made to identify fermented plant extracts that display no cytotoxicity against murine macrophage cells, while still maintaining their biological characteristics. Most of the fermented plant extracts showed reduced cytotoxicity. Among the fourteen plant extracts tested, the fermented plant extract of Scutellaria baicalensis Georgi increased the induction of anti-inflammatory mediators; however, they were still cytotoxic. Interestingly, the plant extract of Artemisia capillaris Thunb. induced a dramatic increase in the induction of anti-inflammatory mediators with no cytotoxicity through fermentation. Our findings suggest that fermented A. capillaris holds promise for use as a valuable natural non-cytotoxic antioxidant and immuno-modulating agent.


Park K.-T.,Milae Resources ML Co. | Oh M.,Milae Resources ML Co. | Nam J.,Milae Resources ML Co. | Ji K.,Milae Resources ML Co. | Han J.-K.,Milae Resources ML Co.
Korean Journal of Food Science and Technology | Year: 2014

A mutant strain of Lactobacillus paracasei (ML-7) was derived by ultraviolet irradiation of the parent L. paracasei LS-2 strain, followed by colony selection on pH 3.5 MRS agar plates, on which L. paracasei LS-2 cannot grow. The L. paracasei ML-7 mutant strain produced an increased amount of organic acids compared to the L. paracasei LS-2 parent strain. Broiler chickens were randomly fed with one of three experimental diets: a basal diet as a control, and basal diets supplemented with 0.3% of the L. paracasei LS-2 and L. paracasei ML-7 strains. When concluding the study, the final body weights of the broilers fed with the supplemented basal diets were found to be significantly higher (1.9% for LS-2 and 6.2% for ML-7) than the control broiler group. Broilers fed with basal diet supplemented with the mutant L. paracasei ML-7 strain showed increased body weight and food intake, compared to broilers fed with the basal diet with or without the parent L. paracasei LS-2 strain. copyright © 2014 The Korean Society of Food Science and Technology.


PubMed | Milae Resources ML Co.
Type: Journal Article | Journal: Comparative immunology, microbiology and infectious diseases | Year: 2010

The objective of this study was to evaluate the immunomodulatory effects of specific bacterial metabolites of Lactobacillus plantarum 10hk2 to induce anti-inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264.7. The effects of the extracellular metabolites of this bacterial strain were examined by dividing them into protein and polysaccharide fractions. A specific protein fraction (8.7 kDa) was found to be a strong IL-10 inducer in LPS-stimulated RAW 264.7 cells and suppressed LPS-induced NF-B induction and inhibited LPS-induced phosphorylation of I-B and p38 MAPK. To the best of our knowledge, this was the first study that investigated the anti-inflammatory effects of an extracellular peptide derived from lactic acid bacteria. In addition, we characterized the inhibitory mode of this molecule in the induction of proinflammatory cytokines. Based on the findings presented in this study, this molecule holds promise for use as an agent to modulate inflammation related diseases.

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