Meir R.,Kimron Veterinary Institute |
Krispel S.,Migal |
Simanov L.,Kimron Veterinary Institute |
Eliahu D.,Migal |
And 3 more authors.
Viral Immunology | Year: 2012
Infectious bronchitis virus (IBV) is prevented primarily by the use of live attenuated vaccines, which are known to have a limited strain range of protection. Alternative vaccines against the emerging new virus strains can improve control of the disease. The aim of this study was to evaluate the immunogenic potential of two recombinant viral proteins, when administered by eyedrop, without the assistance of a vector. The recombinant S1 (rS1) and N (rN) proteins of the M41 strain expressed in E. coli were tested, and the live attenuated vaccine H120 was used as a positive control. Protection was evaluated by re-isolation of virus from tracheas of vaccinated chickens after challenge with strain M41. After three immunizations, rS1 glycoprotein induced 40% protection, while vaccination with rN provided no protection. Vaccination with rS1, rN, or H120 induced a cellular immune response as demonstrated by in vitro ChIFN-γ production by splenocytes of vaccinated birds. Vaccination with H120, and to a lesser extent rS1, induced HI and virus-specific IgG antibody production. These findings indicate that recombinant viral proteins administered through the mucosal route can evoke an immune response without the assistance of a vector. © Copyright 2012, Mary Ann Liebert, Inc. 2012.
Masaphy S.,MIGAL |
Masaphy S.,Galilée College |
Fungal Ecology | Year: 2013
Morchella species ascocarps (morels) are sought-after edible mushrooms that exhibit pyrophilous behavior, proliferating in fire-affected soils of certain types of forests. The factors governing fruiting in this habitat are poorly understood. An observational approach was used to determine the spatial distribution on preferred micro-sites of black morel fruiting in a forest after a summer fire, subjected to different post-fire forestry management activities. Clearing the burnt tree stumps from the site, compaction of the burnt soil by heavy machinery (bulldozers) and covering the soil with chopped wood created preferred micro-sites for black morel fruiting. Fewer fruit bodies developed on untouched burnt soil, and almost none on non-burnt soil at the same site. These observations enhance understanding the ecological principles underlying the distribution and abundance of morel ascocarp development in natural habitats; such an understanding could contribute to conservation and management of morel fruiting in the wild. © 2013 Elsevier Ltd and The British Mycological Society.
Zohar S.,MIGAL |
Kviatkovski I.,Galilée College |
Masaphy S.,MIGAL |
Masaphy S.,Galilée College
International Biodeterioration and Biodegradation | Year: 2013
Arthrobacter sp. 4Hβ, capable of degrading and utilizing high concentrations of p-nitrophenol (PNP) as sole carbon, nitrogen and energy source, was isolated from agricultural soil by a simple enrichment culture. Bacterial growth was accompanied by PNP disappearance and nitrite accumulation, with optimal degradation conditions of 30°C and pH 8. The degradation pathway was via hydroquinone production. Prolonged acclimation period, lower tolerance and reduced degradation rate were obtained upon culturing the bacteria in the presence of increasing PNP concentrations. Complete and 17% removal of 400 and 800mgl-1 initial PNP concentration, respectively, was obtained within 24h of incubation. This latter inhibition of PNP degradation was overcome by increasing the inoculum size. The data suggest that manipulating inoculum size can increase strain 4Hβ's tolerance to, and degradation of high PNP concentrations, as required in certain bioremediation technologies. © 2012 Elsevier Ltd.
Shahar E.,Migal |
Shahar E.,Hadassah Hebrew University Medical Center |
Gorodetsky R.,Hadassah Hebrew University Medical Center |
Gaberman E.,Hadassah Hebrew University Medical Center |
And 3 more authors.
Vaccine | Year: 2010
Antitumor activity of molecules and cells of the innate immune system has been reported. Here we propose a method for targeting preferred innate immune cells and magnifying their tumoricidal effect at the tumor microenvironment, by modular multiple-component complexes (termed TILTAN). As a model, micro-scale complexes were assembled carrying monoclonal anti-HER2 antibodies, lipopolysaccharide and/or mannose. The complexes showed high binding capacity to HER2-positive cancer cells in vitro, high induction of interleukin-1 RNA transcription by the activated monocytes and ability to mediate monocytes' attachment to HER2-positive cells. TILTAN treatment was found safe in in vivo testing and induced change in interleukin-1 RNA transcription in tumors xenografts. We thus present a new vision of targeting a desired innate immune response to the tumor microenvironment. © 2010 Elsevier Ltd.
Dag A.,Israel Agricultural Research Organization |
Naor A.,Golan Research Institute PO Box 97 Kazrin 12900 Israel |
Ben-Gal A.,Israel Agricultural Research Organization |
Harlev G.,Hebrew University of Jerusalem |
And 6 more authors.
Journal of the Science of Food and Agriculture | Year: 2014
BACKGROUND: Over the last two decades, the area of cultivated super-high-density olive orchards has increased rapidly. Water stress is an important tool in super-high-density orchards to reduce tree growth and promote suitability for overhead mechanical harvesters. Little is known regarding the effect of water stress in super-high-density orchards on oil quality parameters. In this study the effect of irrigation rate on oil quality parameters was evaluated in a six-year-old super-high-density 'Koreneiki' olive orchard for five consecutive seasons. Five water status levels, determined by irrigating in order to maintain various midday stem water potential threshold values (-1.5, -2, -2.5, -3 and -4 MPa), were applied during the oil accumulation stage. RESULTS: The MUFA/PUFA ratio and free fatty acid content generally decreased as a function of increasing tree water stress. In most seasons a reduction in polyphenols was found with decreasing irrigation level. Peroxide value was not affected by the water stress level. CONCLUSION: The present study demonstrates that limiting irrigation and exposure of olive trees to water stress in a super-high-density orchard lowers free fatty acid content and therefore benefits oil quality. However, the decreased MUFA/PUFA ratio and the reduction in polyphenol content that were also found under increased water stress negatively influence oil quality. © 2014 Society of Chemical Industry.
Turgeman T.,Ben - Gurion University of the Negev |
Sitrit Y.,Ben - Gurion University of the Negev |
Danai O.,Edible Mushrooms |
Danai O.,Galilée College |
And 6 more authors.
Agroforestry Systems | Year: 2012
A Tuber melanosporum plantation established in 1994/1995 on Kibbutz Bar'am (in the Upper Galilee, Israel) gradually lost its T. melanosporum mycorrhiza. In 1999, T. aestivum inoculated seedlings were inadvertently introduced into the plantation to fill the gaps between trees. A single T. melanosporum fruit body was found in 1999. Although no truffles were found after 1999 and until 2009, in that year and in 2010, truffles were collected. Morphological and molecular analyses proved these to be T. aestivum. Thus, the intentionally introduced T. melanosporum mycorrhiza was replaced by that of another introduced mycorrhizal fungus, T. aestivum. Local oak species produced higher yields compared to introduced host species known to be good T. melanosporum plant symbionts. The yield was comparable to that reported for young commercial orchards, but the fruiting season was earlier than in Europe. © 2012 Springer Science+Business Media B.V.
Bachner-Hinenzon N.,Migal |
Shlomo L.,Technion - Israel Institute of Technology |
Khamis H.,Technion - Israel Institute of Technology |
Ertracht O.,Western Galilee Hospital |
And 5 more authors.
Echocardiography | Year: 2016
Background: It is challenging to detect small nontransmural infarcts visually or automatically. As it is important to detect myocardial infarction (MI) at early stages, we tested the hypothesis that small nontransmural MI can be detected using speckle tracking echocardiography (STE) at the acute stage. Methods: Minimal nontransmural infarcts were induced in 18 rats by causing recurrent ischemia–reperfusion of the left anterior descending (LAD) coronary artery, followed by a 30-min ligation and by reperfusion. A week later, the scar size was measured by histological analysis. Each rat underwent three echocardiography measurements: at baseline, 1 day post-MI, and 1 week post-MI. To measure the peak circumferential strain (CS), peak systolic CS, radial strain (RS), and time-to-peak (TTP) of the CS, short-axis view of the apex was analyzed by a STE program. The TTP was normalized by the duration of the heart cycle to create percent change of heart cycle. Results: Histological analysis after 1 week showed scar size of 4±6% at the anterior wall. At 24 h post-MI, the peak CS, peak systolic CS, and RS were reduced compared to baseline at the anterior wall due to the MI, and at the adjacent segments—the anterior septum and lateral wall, due to stunning (P<.05). However, only the anterior wall, the genuine damaged segment, showed prolonged TTP vs baseline (baseline 36%, 24 h 48%, P<.05). Conclusion: The TTP of the CS can distinguish between regions adjacent to MI (stunned or tethered) and MI, even in small nontransmural infarcts. © 2016, Wiley Periodicals, Inc.
PubMed | Migal, Technion - Israel Institute of Technology, Western Galilee Hospital and Assaf Harofeh Medical Center
Type: Journal Article | Journal: Echocardiography (Mount Kisco, N.Y.) | Year: 2016
It is challenging to detect small nontransmural infarcts visually or automatically. As it is important to detect myocardial infarction (MI) at early stages, we tested the hypothesis that small nontransmural MI can be detected using speckle tracking echocardiography (STE) at the acute stage.Minimal nontransmural infarcts were induced in 18 rats by causing recurrent ischemia-reperfusion of the left anterior descending (LAD) coronary artery, followed by a 30-min ligation and by reperfusion. A week later, the scar size was measured by histological analysis. Each rat underwent three echocardiography measurements: at baseline, 1day post-MI, and 1week post-MI. To measure the peak circumferential strain (CS), peak systolic CS, radial strain (RS), and time-to-peak (TTP) of the CS, short-axis view of the apex was analyzed by a STE program. The TTP was normalized by the duration of the heart cycle to create percent change of heart cycle.Histological analysis after 1week showed scar size of 46% at the anterior wall. At 24h post-MI, the peak CS, peak systolic CS, and RS were reduced compared to baseline at the anterior wall due to the MI, and at the adjacent segments-the anterior septum and lateral wall, due to stunning (P<.05). However, only the anterior wall, the genuine damaged segment, showed prolonged TTP vs baseline (baseline 36%, 24h 48%, P<.05).The TTP of the CS can distinguish between regions adjacent to MI (stunned or tethered) and MI, even in small nontransmural infarcts.