Mie Prefecture Health and Environment Research Institute

Mie, Japan

Mie Prefecture Health and Environment Research Institute

Mie, Japan
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Iwade Y.,Mie Prefecture Health and Environment Research Institute | Yamazaki W.,University of Miyazaki | Gondaira F.,Denka Seiken Co. | Vuddhakul V.,Prince of Songkla University | And 2 more authors.
Journal of Food Protection | Year: 2014

Although thermostable direct hemolysin-producing (tdh+) Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh+ V. parahaemolyticus remains challenging due to its low densities in the environment. In this study, we developed a most-probable-number (MPN)-based procedure designated A-IS1-LAMP, in which an immunomagnetic separation (IMS) technique targeting as many as 69 established K antigens and a loop-mediated isothermal amplification (LAMP) assay targeting the thermostable direct hemolysin (tdh) gene were applied in an MPN format. Our IMS employed PickPen, an eight-channel intrasolution magnetic particle separation device, which enabled a straightforward microtiter plate-based IMS procedure (designated as PickPen-IMS). The ability of the procedure to quantify a wide range of tdh+ V. parahaemolyticus levels was evaluated by testing shellfish samples in Japan and southern Thailand, where shellfish products are known to contain relatively low and high levels of total V. parahaemolyticus, respectively. The Japanese and Thai shellfish samples showed, respectively, relatively low (<3 to 11 MPN/10 g) and considerably higher (930 to 110,000 MPN/10 g) levels of tdh+ V. parahaemolyticus, raising concern about the safety of Thai shellfish products sold to domestic consumers at local morning markets. LAMP showed similar or higher performance than conventional PCR in the detection and quantification of a wide range of tdh+ V. parahaemolyticus levels in shellfish products. Whereas a positive effect of PickPen-IMS was not observed in MPN determination, PickPen-IMS was able to concentrate tdh+ V. parahaemolyticus 32-fold on average from the Japanese shellfish samples at an individual tube level, suggesting a possibility of using PickPen-IMS as an optional tool for specific shellfish samples. The A-IS1-LAMP procedure can be used by any health authority in the world to measure the tdh+ V. parahaemolyticus levels in shellfish products. © International Association for Food Protection.


PubMed | Mie Prefecture Health and Environment Research Institute and Mie University
Type: Journal Article | Journal: The Journal of dermatology | Year: 2015

Rickettsia diseases, including Japanese spotted fever (JSF), are serious infections. Delayed diagnosis occasionally results in life-threatening liver disorders and disseminated intravascular coagulation (DIC). Because of the shortness of the latent period, serological diagnosis is not preferable for early diagnosis of JSF. Until now, a polymerase chain reaction (PCR)-based diagnosis method has been used for early diagnosis, and the sensitivity reaches as high as 90% using skin biopsy samples as we previously reported. On the other hand, the sensitivity of the same PCR method using blood samples is limited at less than 50%. In the present study, using peripheral blood samples, we developed a novel diagnostic method for JSF using a Rick PCR system with original PCR primers, showing improved sensitivity compared with the conventional nested PCR. It may constitute a preferable diagnostic tool for early and sensitive diagnosis of Rickettsia infection.


Okano H.,Suzuka General Hospital | Akachi S.,Mie Prefecture Health and Environment Research Institute | Nakano T.,Fujita Health University Nanakuri Sanatorium | Okamoto H.,Jichi Medical University
Acta Hepatologica Japonica | Year: 2014

The source of European-type subgenotype 3e/3sp hepatitis E virus (HEV) strain that is prevalent in hepatitis E patients in Mie, Japan, remained unknown. The present analyses of stored RNA samples that had been extracted from liver tissues obtained from 85 slaughtered pigs in Mie in 2007, revealed the presence of two HEV strains (swJ-Mie07-37L and swJ-Mie07-80L). The swJ-Mie07-80L strain was classified into subgenotype 3b/3jp, while swJ-Mie07-37L belonged to 3e/3sp and shared high nucleotide sequence identities of 98.399.2% with the 3e/3sp strains recovered from hepatitis E patients in Mie during 2004-2012. These results suggest that pigs are important reservoirs for HEV infection even in Mie, although the hepatitis E patients did not recall consuming raw pig liver/intestine before the disease onset. © 2014 The Japan society Hepatology.


PubMed | Mie Prefecture Health and Environment Research Institute, University of Miyazaki, Kyoto University, Denka Seiken Co. and Prince of Songkla University
Type: Evaluation Studies | Journal: Journal of food protection | Year: 2014

Although thermostable direct hemolysin-producing (tdh(+)) Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh(+) V. parahaemolyticus remains challenging due to its low densities in the environment. In this study, we developed a most-probable-number (MPN)-based procedure designated A-IS(1)-LAMP, in which an immunomagnetic separation (IMS) technique targeting as many as 69 established K antigens and a loop-mediated isothermal amplification (LAMP) assay targeting the thermostable direct hemolysin (tdh) gene were applied in an MPN format. Our IMS employed PickPen, an eight-channel intrasolution magnetic particle separation device, which enabled a straightforward microtiter plate-based IMS procedure (designated as PickPen-IMS). The ability of the procedure to quantify a wide range of tdh(+) V. parahaemolyticus levels was evaluated by testing shellfish samples in Japan and southern Thailand, where shellfish products are known to contain relatively low and high levels of total V. parahaemolyticus, respectively. The Japanese and Thai shellfish samples showed, respectively, relatively low (< 3 to 11 MPN/10 g) and considerably higher (930 to 110,000 MPN/10 g) levels of tdh(+) V. parahaemolyticus, raising concern about the safety of Thai shellfish products sold to domestic consumers at local morning markets. LAMP showed similar or higher performance than conventional PCR in the detection and quantification of a wide range of tdh(+) V. parahaemolyticus levels in shellfish products. Whereas a positive effect of PickPen-IMS was not observed in MPN determination, PickPen-IMS was able to concentrate tdh(+) V. parahaemolyticus 32-fold on average from the Japanese shellfish samples at an individual tube level, suggesting a possibility of using PickPen-IMS as an optional tool for specific shellfish samples. The A-IS(1)-LAMP procedure can be used by any health authority in the world to measure the tdh(+) V. parahaemolyticus levels in shellfish products.


Kondo M.,Mie University | Akachi S.,Mie Prefecture Health and Environment Research Institute | Ando K.,Mie University | Nomura T.,Mie Prefectural Shima Hospital | And 2 more authors.
Journal of Dermatology | Year: 2016

Two Japanese siblings visited the Cook Islands on business and stayed for 2 months. The sister developed a high fever, arthralgia, erythema and leg edema on the day after returning to Japan. The brother also developed neck and joint pain on the day following the sister's onset. Subsequently, his erythematous lesions spread over his whole body. Chikungunya virus was detected from the sister's blood and urine by specific reverse transcription polymerase chain reaction, but not in the brother's samples. Retrospectively, his history of Chikungunya fever was confirmed by the presence of the anti-Chikungunya virus immunoglobulin (Ig)M and IgG antibodies using the specific enzyme-linked immunoassay. In Japan, no autochthonous case of Chikungunya fever was reported previously. We should give attention to the imported infectious diseases for epidemic prevention. This report warns about the danger of the imported infectious diseases, and also suggests that covering the topic of infectious disease in the world is critical to doctors as well as travelers. © 2016 Japanese Dermatological Association.


Kondo M.,Ise Municipal General Hospital | Kurokawa I.,Mie University | Yamanaka K.-I.,Mie University | Akachi S.,Mie Prefecture Health and Environment Research Institute | Nishii M.,Ise Municipal General Hospital
International Journal of Dermatology | Year: 2010

Background An 81-year-old female experiencing high fever, fatigue, and loss of appetite was admitted to our hospital and diagnosed with acute cholecystitis. Her condition did not improve and an eschar and erythema subsequently appeared. We then diagnosed Japanese spotted fever (JSF). She recovered immediately after the administration of minocycline. This case differed from other cases because the patient had a remarkably acute hepatic failure. Methods Considering that the present case might be associated with other factors, we performed a repeat polymerase chain reaction (PCR) test on the patient's blood that had been collected on admission and stored. Results Epstein-Barr virus (EBV) was detected in her blood by PCR. Conclusion We consider this case might be associated with EBV. © 2010 The International Society of Dermatology.


Takahashi H.,Mie Prefecture Health and Environment Research Institute
Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases | Year: 2013

We examined the efficacy and safety of inactivated influenza vaccine when the amount of HA influenza vaccination in children was increased to the dose recommended by the WHO. The purpose of this study was to obtain basic evidence to review the vaccination dose in Japanese children. HA influenza vaccine produced by the Research Foundation for Microbial Diseases of Osaka University (Biken) licenced in Japan was administered through vaccination at the international dose, and split HA influenza vaccine produced by Sanofi Pasteur corp. (Sanofi) was used as control. Children from 6 months to less than 13 years of age were registered, and vaccinated with doses of 0.25 mL or 0.5 mL. Clinical symptoms during the influenza season were monitored to investigate vaccine efficacy, and information on adverse reactions was collected to evaluate safety profile. Paired serum HI and NT antibody titers were measured at pre first dose and post second dose of vaccination. Both HI and NT antibody titers for H1N1 subtype were satisfactory elevated after administration of both vaccines. Elevation of the NT antibody titer for the H3N2 subtype was observed for both vaccines, but the H3N2 HI antibody titer for the Biken vaccine was not so high. For the subtype B virus, the NT titer had a better response than the HI titer for both vaccines. As only the H1N1 virus was prevalent in the area during the study period, we performed factor analysis concerning influenza contraction only for the H1N1 antibody titer. An HI titer of 1 : 40 or more at post-vaccination was a significant factor to lower the risk of influenza contraction. The relative risk for fever among children with an HI titer of 1 : 20 or less was significantly higher than those with an HI titer of 1 : 40 or more. Children with a higher HI titer had better prevention against fever, so that both vaccines were considered to be effective. As for the appearance of adverse reactions, both vaccines were considered to be safe. From the above-mentioned results, vaccination with the Japanese Biken vaccine at an international dose was thought to be an effective and safe procedure.


Kurokawa I.,Meiwa Hospital | Kondo M.,Mie University | Kondo M.,Ise Municipal General Hospital | Akachi S.,Mie Prefecture Health and Environment Research Institute
Journal of Infection and Chemotherapy | Year: 2013

We studied the suitability of a PCR method using samples of skin and whole blood and serological tests for the early diagnosis of Japan spotted fever (JSF) in its acute and convalescent stages and compared the advantages and disadvantages of these different diagnostic methods. In the acute stage, the percentage of positive results was 91.2 % for the PCR method using skin samples, 52.3 % for the PCR method using whole blood samples, and 40.4 % for the serological tests with IgM. In the convalescent stage, paired serum showed positive results (IgM, 98.5 %; IgC, 94.0 %). The PCR method using samples of skin (eschar) is the most sensitive, specific, and suitable method for promptly and accurately diagnosing JSF in the early stage. Therefore, this method is recommended for early definite diagnosis of JSF in the critical stage. © 2012 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases.


Kondo M.,Ise Municipal General Hospital | Nishii M.,Ise Municipal General Hospital | Gabazza E.C.,Mie University | Kurokawa I.,Mie University | Akachi S.,Mie Prefecture Health and Environment Research Institute
International Journal of Dermatology | Year: 2010

Background: The diagnosis of Japan spotted fever (JSF) is very difficult in some cases. The initial diagnosis of JSF is very important to treat. Methods: We report nine cases of Japan spotted fever (JSF) with variable clinical features diagnosed at our hospital in 2008. Results: Concerning clinical symptom, the most frequent symptoms were fever (8/9) and erythema of the whole body (8/9), followed by eschar (4/9). Palmar erythema, vomiting, and headache were observed in two cases. Purpura and lymph node swelling were observed in one case. Complication with Disseminated intravascular coagulation (DIC) was observed in one case. Laboratory findings revealed elevated plasma level of C-reactive protein (CRP) and liver dysfunction in all cases, and decreased platelet (7/9). Interestingly, all patients had a history of presumed infection in the Southern area of Miya River, where wild Japanese deer with ticks (vector of Rickettsia japonica) may reside. Conclusion: Different procedures are performed to make a diagnosis of JSF. For an initial definite diagnosis and adequate treatment of JSF, PCR of samples taken from blood, and skin biopsy from erythema and eschar lesions are necessary. Paired serum to measure the titers of antibody against R. japonica is also important. © 2010 The International Society of Dermatology.


PubMed | Mie Prefecture Health and Environment Research Institute, Mie Prefectural Shima Hospital and Mie University
Type: Journal Article | Journal: The Journal of dermatology | Year: 2016

Two Japanese siblings visited the Cook Islands on business and stayed for 2 months. The sister developed a high fever, arthralgia, erythema and leg edema on the day after returning to Japan. The brother also developed neck and joint pain on the day following the sisters onset. Subsequently, his erythematous lesions spread over his whole body. Chikungunya virus was detected from the sisters blood and urine by specific reverse transcription polymerase chain reaction, but not in the brothers samples. Retrospectively, his history of Chikungunya fever was confirmed by the presence of the anti-Chikungunya virus immunoglobulin (Ig)M and IgG antibodies using the specific enzyme-linked immunoassay. In Japan, no autochthonous case of Chikungunya fever was reported previously. We should give attention to the imported infectious diseases for epidemic prevention. This report warns about the danger of the imported infectious diseases, and also suggests that covering the topic of infectious disease in the world is critical to doctors as well as travelers.

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