Okamoto T.,Mie University |
Akita N.,Suzuka University of Medical Science |
Kawamoto E.,Mie University |
Hayashi T.,Mie Prefectural College of Nursing |
And 2 more authors.
Experimental Cell Research | Year: 2014
The gap junction proteins connexin32 (Cx32), Cx37, Cx40, and Cx43 are expressed in endothelial cells, and regulate vascular functions involving inflammation, vasculogenesis and vascular remodeling. Aberrant Cxs expression promotes the development of atherosclerosis which is modulated by angiogenesis; however the role played by endothelial Cxs in angiogenesis remains unclear. In this study, we determined the effects of endothelial Cxs, particularly Cx32, on angiogenesis. EA.hy926 cells that had been transfected to overexpress Cx32 significantly increased capillary length and the number on branches compared to Cx-transfectant cells over-expressing Cx37, Cx40, and Cx43 or mock-treated cells. Treatment via intracellular transfer of anti-Cx32 antibody suppressed tube formation of human umbilical vein endothelial cells (HUVECs) compared to controls. In vitro wound healing assays revealed that Cx32-transfectant cells significantly increased the repaired area while anti-Cx32 antibody-treated HUVECs reduced it. Ex vivo aorta ring assays and in vivo matrigel plaque assays showed that Cx32-deficient mice impaired both vascular sprouting from the aorta and cell migration into the implanted matrigel. Therefore endothelial Cx32 facilitates tube formation, wound healing, vascular sprouting, and cell migration. Our results suggest that endothelial Cx32 positively regulates angiogenesis by enhancing endothelial cell tube formation and cell migration. © 2013 Elsevier Inc.
Suzuki M.,Hamamatsu University School of Medicine |
Tatsumi A.,Hamamatsu University School of Medicine |
Otsuka T.,Hamamatsu University School of Medicine |
Kikuchi K.,Hamamatsu University School of Medicine |
And 8 more authors.
American Journal of Alzheimer's Disease and other Dementias | Year: 2010
Tactile massage is a soft massage that improves physical relaxation and psychological well-being. The purpose of this study was to clarify the effects of a 6-week tactile massage on changes in physical and mental function, symptoms of behavioral and psychological symptoms of dementia (BPSD) among elderly patients with dementia. In addition, chromogranin A (CgA) levels as an index of stress examined the effects of tactile massage intervention. A tactile massage group consisting of elderly patients with dementia received tactile massage therapy a total of 30 times each for about 20 minutes between 16:00 and 17:00 hours. In the control group, the mean scores for "intellectual" and "emotional function" score decreased significantly after 6 weeks (P <.05); however, no change was observed in the tactile massage group. Both the "aggressiveness" score (P <.05) and CgA levels decreased significantly after 6 weeks in the tactile massage group. These results suggest that tactile massage reduces aggressiveness and stress level in patients with dementia. © The Author(s) 2010.
Chiba R.,Jichi Medical University |
Miyamoto Y.,University of Tokyo |
Funakoshi A.,Mie Prefectural College of Nursing
Journal of Mental Health | Year: 2014
Background: Benefit finding is defined as finding benefits through the struggle with adversity. Aim: This study explored benefit finding at different stages of recovery among people with severe mental illness in Japan. Methods: A cross-sectional questionnaire survey, which contained both open-ended questions regarding benefit finding and the Recovery Assessment Scale (RAS), was conducted. Of the responses received from 193 (61%) of 319 individuals with mental illness, responses about benefit finding from 94 questionnaires was analyzed using content analysis (males: 57%; females: 43%; average age: 45 years). Each response about benefit finding was classified into one of three groups according to the stages of recovery by their RAS score (i.e. low, middle or high). Results: The group with higher recovery scores provided more examples of benefit finding, although almost a quarter of examples of benefit finding were provided by the low-RAS group. Different benefit finding characteristics were found between groups of people at different stages of recovery. Conclusion: While individuals with higher recovery scores are likely to find a variety of benefits, even individuals with lower recovery scores are capable of benefit finding. © 2014 Informa UK Ltd.
Ogata S.,Osaka City University |
Ogata S.,Osaka University |
Hayashi C.,Osaka City University |
Sugiura K.,Senri Kinran University |
And 3 more authors.
PLoS ONE | Year: 2015
Depressive state has been reported to be significantly associated with higher-level functional capacity among community-dwelling elderly. However, few studies have investigated the associations among people with long-term care requirements. We aimed to investigate the associations between depressive state and higher-level functional capacity and obtain marginal odds ratios using propensity score analyses in people with long-term care requirements. We conducted a cross-sectional study based on participants aged ≥65 years (n = 545) who were community dwelling and used outpatient care services for long-term preventive care. We measured higher-level functional capacity, depressive state, and possible confounders. Then, we estimated the marginal odds ratios (i.e., the change in odds of impaired higher-level functional capacity if all versus no participants were exposed to depressive state) by logistic models using generalized linear models with the inverse probability of treatment weighting (IPTW) for propensity score and design-based standard errors. Depressive state was used as the exposure variable and higher-level functional capacity as the outcome variable. The all absolute standardized differences after the IPTW using the propensity scores were <10% which indicated negligible differences in the mean or prevalence of the covariates between non-depressive state and depressive state. The marginal odds ratios were estimated by the logistic models with IPTW using the propensity scores. The marginal odds ratios were 2.17 (95%CI: 1.13-4.19) for men and 2.57 (95%CI: 1.26-5.26) for women. Prevention of depressive state may contribute to not only depressive state but also higher-level functional capacity. © 2015 Ogata et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funakoshi A.,Mie Prefectural College of Nursing |
Miyamoto Y.,University of Tokyo
Psychiatry and Clinical Neurosciences | Year: 2015
Aims Hikikomori is a new psychosociological phenomenon among youth, of almost complete withdrawal from social interaction, and it has received considerable attention in community mental health in Japan. The aims of the present study were to identify the influential factors of family difficulties of parents who use support services for children with hikikomori, and compare them between fathers and mothers. Methods Data were collected from 110 parents (55 couples) of children with hikikomori with regard to family difficulties, quality of life, and depression variables via self-report questionnaires. To assess the influential factors of Family Difficulties for parents with children with hikikomori, hierarchical multiple linear regression analysis was carried out for gender. Results While 94.5% of mothers received some kind of family support, only 61.9% of fathers received it. For both genders, the number of services that the fathers received was significantly correlated with marital cooperation, and the number of services that the mothers received was significantly correlated with support resource utilization. Conclusions It is necessary for fathers to receive more support, and it is important for professionals to encourage parents to address their difficulties together. © 2014 The Authors.