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Navegantes K.C.,Federal University of Para | Albuquerque R.F.V.,Federal University of Para | Dalla-Santa H.S.,Midwest State University | Soccol C.R.,Federal University of Parana | Monteiro M.C.,Federal University of Para
Food and Agricultural Immunology | Year: 2013

The biological properties of Agaricus brasiliensis mainly include immunostimulating and antitumour activities. This study evaluated the immunomodulating effect of A. brasiliensis mycelium (LPB) and its exopolysaccharides (EPS) on immunological parameters, such as phagocytosis of Candida albicans and nitric oxide (NO) production by infected macrophages, splenocytes proliferation in response to C. albicans. It also assessed the effects of the intake of LPB on the number of lymphocytes in the lymph node and spleen of treated animals and the TNF-α production. It was showed that the LPB and EPS had opposite effects; LPB had antiproliferative action and also reduced the NO levels produced by macrophages. However, the EPS showed an immune-stimulating effect potentiating the splenocytes proliferation and NO production from macrophages after stimulation with C. albicans. In addition, it also protected the macrophages from death induced by yeast. These data confirmed the immunomodulatory properties of LPB and its polysaccharides. © 2012 Taylor & Francis. Source


Guerra F.,University of Sao Paulo | Trevizam A.R.,Midwest State University | Fior R.C.,University of Sao Paulo | Muraoka T.,University of Sao Paulo
Scientia Agricola | Year: 2014

Large areas of land are nowadays contaminated by heavy metals and, it is therefore, important to monitor their levels in soils. Vegetables act as transfer mechanisms of such contaminants from soils to higher levels in the food chain. In this study, we aimed to evaluate the effectiveness of chemical extractants by the L-value method for Cd phytoavailability using the 109Cd radionuclide. In a greenhouse experiment, rocket plants (Eruca sativa L.) were cultivated in pots with samples from Typic Hapludox and Typic Quartzipsamment soils. Cadmium concentrations ranging from 0 to 16 mg kg-1 were added to a 200 mL solution containing 148 kBq 109Cd. The available Cd in the soil was extracted by DTPA, Mehlich-1, Mehlich-3, and a mixture of organic acids (acetic, citric, lactic, and oxalic acids). Cd concentrations were determined by atomic absorption spectrophotometry, and 109Cd radionuclide activity was measured by low-level β-counting. The dry matter yield was not influenced by Cd rates, but the Cd content and accumulation in shoots had a positive linear correlation. Generally, Cd was extracted in higher quantities by Mehlich-1 followed by DTPA, Mehlich-3, and organic acids. A linear correlation was found between the chemical extractants and Cd accumulation in shoots for both soils. According to the L Ratio, the extractants based on strong acids and chelating agents presented low efficiency regarding Cd phytoavailability. The organic acids, which presented values close to the L-value, may provide a promising method for evaluating environmental contaminants. © 2014, Scientia Agricola. All rights reserved. Source


Correa C.S.,Federal University of Rio Grande do Sul | Laroche D.P.,University of New Hampshire | Cadore E.L.,Federal University of Rio Grande do Sul | Reischak-Oliveira A.,Federal University of Rio Grande do Sul | And 8 more authors.
International Journal of Sports Medicine | Year: 2012

The objective of the present study was to evaluate and compare the neuromuscular, morphological and functional adaptations of older women subjected to 3 different types of strength training. 58, healthy women (67±5 year) were randomized to experimental (EG, n=41) and control groups (CG, n=17) during the first 6 weeks when the EG group performed traditional resistance exercise for the lower extremity. Afterwards, EG was divided into three specific strength training groups; a traditional group (TG, n=14), a power group (PG, n=13) that performed the concentric phase of contraction at high speed and a rapid strength group (RG, n=14) that performed a lateral box jump exercise emphasizing the stretch-shortening-cycle (SSC). Subjects trained 2 days per week through the entire 12 weeks. Following 6 weeks of generalized strength training, significant improvements occurred in EG for knee extension one-repetition (1RM) maximum strength (+19%), knee extensor muscle thickness (MT, +15%), maximal muscle activation (+44% average) and onset latency (31% average) for vastus lateralis (VL), vastus medialis (VM) and rectus femoris (RF) compared to CG (p<0.05). Following 6 more weeks of specific strength training, the 1RM increased significantly and similarly between groups (average of +21%), as did muscle thickness of the VL (+25%), and activation of VL (+44%) and VM (+26%). The onset latency of RF (TG=285±109ms, PG=252±76ms, RG=203±43ms), reaction time (TG=366±99ms, PG=274±76ms, RG=201±41ms), 30-s chair stand (TG=18±3, PG=18±1, RG=21±2) and counter movement jump (TG=8±2cm, PG=10±3cm, RG=13±2cm) was significantly improved only in RG (p<0.05). At the end of training, the rate of force development (RFD) over 150ms (TG=2.3±9.8N·s1, PG=3.3±3.2N·s1, RG=3.8±6.8N·s 1, CG=2.3±7.0N·s1) was significantly greater in RG and PG than in TG and CG (p<0.05). In conclusion, rapid strength training is more effective for the development of rapid force production of muscle than other specific types of strength training and by consequence, better develops the functional capabilities of older women. © Georg Thieme Verlag KG Stuttgart . New York. Source


Zanette C.M.,Midwest State University | Dalla Santa O.R.,Midwest State University | Bersot L.S.,Federal University of Parana
International Food Research Journal | Year: 2015

The objective of this study was to evaluate the effect of a bacteriocinogenic starter culture against L. monocytogenes in artificially contaminated sausages. During the maturation of sausages the following procedures were carried out: physical-chemical analysis, lactic acid bacteria counts, and enumeration of L. monocytogenes. In the treatment inoculated only with a pool of L. monocytogenes, the multiplication of the pathogen was verified during the 19 days of maturation and the increase in the pathogen population was 2.01 log CFU.g-1. In the treatments inoculated with starter cultures of bacteriocinogenic and bacteriocin-negative L. plantarum, we observed a 1.7 log CFU.g-1 reduction in levels of L. monocytogenes during the sausages maturation. However, no significant differences (P > 0.01) were observed between the treatments inoculated with starter cultures. The pH values of the control treatment remained almost constant at 6.07 during the maturation, differing significantly (P < 0.01) from treatments inoculated with starter cultures. © All Rights Reserved. Source


Cunha M.T.,Midwest State University | Rodrigues P.R.P.,Midwest State University
Materials Science Forum | Year: 2015

The main aim of this paper is to study copper ions electroplating, evaluating the cathodic current efficacy, from cyanide ion free alkaline baths, stabilized by different complexant agents for the Cu2+ ions. The cathodic current efficacy was assessed through quartz microbalance measurements, employing different current modulations. Results revealed that the pulsed current application has great influence on the cathodic current efficacy during electroplating in the baths under study. © (2015) Trans Tech Publications, Switzerland. Source

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