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Fukui-shi, Japan

Kumar V.,Microbiology Section | Singh Solanki A.,Plant Wealth Sector | Sharma S.,Motilal Nehru National Institute of Technology
Thai Journal of Agricultural Science | Year: 2011

The yield and nutrient uptake of Plantago ovata were studied using dual inoculation with AM fungi (Glomus fasciculatum) and Azotobacter chroococcum. The experiment was conducted for two consecutive years, with two levels of chemical fertilizers (F1: N-P-K: 24-24-32 kg ha -1) and (F2: N-P-K: 30-30-40) kg ha -1). Inoculation with the AM fungi and Azotobacter chroococcum generated encouraging results; seed yield (1120.7 kg ha -1), husk yield (297.1 kg ha -1), plant height (40.4 cm), spike plant-1 (33.6), and 1000 seeds weight (1.793 g) were highest with treatment T12 (F2 fertilizer level + both bioinoculants) followed by treatment T9 (F1 fertilizer level + both bioinoculants), T10 (F2 + A. chroococcum), T11 (F2 + AM fungi), as compared to T1 (control), T2-T4 (bioinoculants) and T5-T6 (F1 and F2 fertilizer levels). The nitrogen uptake was more in Azotobacter treated plants, while higher P and K uptake was attained in AM fungi inoculated plants. The survival count of inoculated bacteria was highest on 60 days after inoculation and declined thereafter. Economic analysis revealed the net profit from seed and husk was highest in T9 using dual inoculation of microbes with 80% of recommended dose of fertilizer followed by T12 and T10. Source

Roda A.,University of Bologna | Roda A.,Italian National Institute of Biosystems and Biostructures | Mirasoli M.,University of Bologna | Dolci L.S.,University of Bologna | And 5 more authors.
Analytical Chemistry | Year: 2011

A simple and versatile analytical device designed to perform, even simultaneously, different types of bioassays has been developed and optimized. A transparent microfluidics-based reaction chip, where analytes were quantitatively detected by means of biospecific reactions and chemiluminescence detection, was placed in contact with a thermoelectrically cooled CCD sensor through a fiber optic taper. Such a lensless contact imaging configuration combined adequate spatial resolution and high light collection efficiency within a small size portable device. The miniaturization of the reaction chamber ensured short analysis times (in the minutes range), while the use of chemiluminescence detection provided wide signal dynamic range and high detectability, down to attomole levels of protein and femtomole levels of nucleic acid analytes. A model hybrid panel test was realized by combining an enzyme assay for alkaline phosphatase activity, a nucleic acid hybridization assay for Parvovirus B19 DNA, and an immunoassay for horseradish peroxidase as a model antigen. The successful simultaneous quantification of the three targets demonstrated that a range of analytes, from enzymes to antigens, antibodies, and nucleic acids, can be measured in a single run, thus enabling the realization of a complete, personalized diagnostic panel test for early diagnosis of a given disease and patient follow-up. © 2011 American Chemical Society. Source

Thongkoom P.,Microbiology Section
Journal of the Medical Association of Thailand = Chotmaihet thangphaet | Year: 2012

From 1999 to 2009, a total of 10,470 clinical enterococcal strains from patients in Rajavithi Hospital were isolated. Of these, 201 (1.9%) vancomycin-resistant enterococci (VRE) including 199 (99.0%) Enterococcus faecium and 2 (1.00%) Enterococcus faecalis were found. The incidence of VRE was 1.8% in 1999, 3.3% in 2000, 5.1% in 2001, 1.0% in 2002, 0.0% in 2003 and 2004, 1.1% in 2005, 4.7% in 2006, 1.9% in 2007, 2.2% in 2008 and 0.9% in 2009. Seventy-one VRE isolates were classified to VanA phenotype (69 VanA E. faecium and 2 VanA E. faecalis) and 130 were classified to VanB phenotype (VanB E. faecium). The rate of inpatient departments (IPD)-associated VRE (199 (99.0%) VRE isolates) was significantly greater than the incidence of outpatient departments (OPD)-VRE (2 (1.0%) VRE isolates). VRE were found in medical (33.7%), ICUs (15.6%) and surgical (15.1%) wards. VRE were mostly found in urine, 64.2%, following in pus, blood, genital and sputum specimens, 21.9%, 9.0%, 3.5% and 1.4%, respectively. VRE, especially vancomycin resistant E. faecium, were multidrug-resistant (resistance to ampicillin, tetracycline, norfloxacin, ciprofloxacin, erythromycin and gentamicin). All strains of VRE were fully susceptible to linezolid. Source

Varalakshmi S.,National Dairy Research Institute | Kirubaharan J.J.,Microbiology Section
Research Journal of Biotechnology | Year: 2014

The effect of Cyclophosphamide (CY) on the immune status of birds was studied by administering the chickens with live Newcastle disease vaccine. Normal and immunosuppressed chickens were vaccinated with the Thermostable live Newcastle disease vaccine by ocular-nasal route at 10th day of age. The immune suppressed chickens had significantly low serum antibody titers and histopathological studies showed that there is depletion of bursal tissue in the CY treated chickens compared to CY untreated chickens. There is no significant difference in the cell mediated immunity between ablated and non ablated group. This study concludes that there is suppression of only humoral immunity by administering the cyclophosphamide and there is no effect on cell mediated immunity. CY will be used to study the role of cell mediated immunity and mucosal immunity in the absence of humoral immunity. Source

Cobo F.,Microbiology Section
Human Papillomavirus Infections: From the Laboratory to Clinical Practice | Year: 2012

Human papillomavirus (HPV) is one of the most common causes of sexually transmitted diseases worldwide, both in men and women. Human papillomavirus infections provides the scientific background needed to understand the natural history and pathogenesis of HPV infection and offers discussion of its clinical features, diagnosis, treatment and prevention. The book begins with chapters covering the epidemiology, virology, history and transmission of the infection, as well as its pathogenesis and clinical features. Following a discussion of the relationship between HPV and cancer, chapters in the second half of the book look at diagnosis, testing and treatment. The book concludes with detailed coverage of the prevention of HPV through worldwide vaccination programmes. © 2012 Woodhead Publishing Limited All rights reserved. Source

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