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Ben Kahla I.,Veterinary Research Institute of Tunisia | Ben Kahla I.,Microbiology and Immunology Laboratory | Boschiroli M.L.,Bacterial Zoonoses Unit Animal Health Laboratory French Agency for Food | Souissi F.,Veterinary Research Institute of Tunisia | And 4 more authors.
African Health Sciences | Year: 2011

Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing. Results: A total of five (4.9%) M. bovis strains exhibiting three different genotypes were isolated. Conclusion: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis. Source


Ben-Selma W.,Microbiology and Immunology Laboratory | Harizi H.,Microbiology and Immunology Laboratory | Boukadida J.,Microbiology and Immunology Laboratory
Clinical and Vaccine Immunology | Year: 2011

For rapid diagnosis and discrimination between active tuberculosis (TB) and other pulmonary diseases, we evaluated the clinical usefulness of detection of serum immunoglobulin IgG and IgM antibodies raised against mycobacterial 38-kDa, 16-kDa, and 6-kDa antigens by a commercial rapid immunochromatographic IgG/IgM test (Standard Diagnostics, South Korea) in 246 serum samples from three groups of patients: (i) 171 patients with active TB (128 with pulmonary TB [pTB] and 43 with extrapulmonary TB [epTB]), (ii) 73 patients with pulmonary non-TB diseases, and (iii) two leprosy patients. The sensitivities of IgG and IgM in patients with active TB (pTB and epTB) were 68.4% and 2.3%, respectively. IgG had the best performance characteristics, with sensitivities of 78.1% and 39.5% in sera from patients with active pTB and epTB, respectively, and a specificity of 100%. The sensitivities of IgM were poor and were similar for pTB and epTB (2.3%). In contrast, specificity was very elevated (100%). The combination of IgG with IgM did not improve its sensitivity. IgG-mediated responses against the mycobacterial 38-kDa, 16-kDa, and 6-kDa antigens might constitute a clinically useful tool for presumptive diagnosis and discrimination of active pTB from other pulmonary diseases. Moreover, based on its simplicity and rapidity of application, it could be a screening tool for active pTB in poorly equipped laboratories. Copyright © 2011, American Society for Microbiology. All Rights Reserved. Source


Azouzi F.,Microbiology and Immunology Laboratory | Chahed C.,Microbiology and Immunology Laboratory | Marzouk M.,Microbiology and Immunology Laboratory | Ferjani A.,Microbiology and Immunology Laboratory | And 4 more authors.
Case Reports in Women's Health | Year: 2015

Lactococcus lactis cremoris is rarely involved in human pathology. A thirty two-year old pregnant woman with premature rupture of membrane history presented with chorioamnionitis due to L. lactis cremoris. She underwent an emergency caesarian section and was treated with antibiotics including the association of amoxicillin and clavulanic acid. She was completely recovered. This is the first case to our knowledge of chorioamnionitis due to this organism. © 2015, Elsevier Inc. All rights reserved. Source


Ben-selma W.,Microbiology and Immunology Laboratory | Harizi H.,Microbiology and Immunology Laboratory | Marzouk M.,Microbiology and Immunology Laboratory | Ben Kahla I.,Microbiology and Immunology Laboratory | And 3 more authors.
Diagnostic Microbiology and Infectious Disease | Year: 2010

The purpose of the present study was to evaluate the clinical usefulness of detection of serum immunoglobulin A (IgA), IgG, and IgM antibodies raised against the mycobacterial A60 antigen for the diagnosis and discrimination of active tuberculosis (TB) from other pulmonary diseases. Three commercially available ELISA kits (IgA, IgG, and IgM) (ANDA Biologicals, Strasbourg, France) were evaluated simultaneously in 246 serum samples from 3 groups of patients: group I, 171 patients with active TB (128 pulmonary TB and 43 extrapulmonary TB); group II, 73 patients with pulmonary non-TB diseases; and group III, 2 leprosies patients. The sensitivities of tests ranged from 31.3% (IgA) to 94% (IgG) in pulmonary TB patients and from 21% (IgA) to 84% (IgG) in extrapulmonary TB patients. The specificities of assays varied from 92% (IgG) to 96% (IgA) in the pulmonary non-TB group. Combination of IgG with IgA and/or IgM does not improve its sensitivity. Clinical use of the A60-based serodiagnostic IgG assay is of great value for the rapid diagnosis and discrimination between active TB and pulmonary non-TB diseases. Moreover, this test could be used to increase diagnostic accuracy, especially for smear-negative TB cases, which are difficult to diagnose. © 2010 Elsevier Inc. Source

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