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Kraków, Poland

Budak A.,Jagiellonian University | Bogusz B.,Microbiological Laboratory | Tokarczyk M.,Jagiellonian University | Trojanowska D.,Jagiellonian University
Mycoses | Year: 2013

Superficial fungal infections due to dermatophytes are common over the world and their frequency is constantly increasing. The aim of our study was to discuss fungal infections with frequency of occurrence, clinical stages and aetiology in patients admitted to dermatological ward and microbiological laboratory of the specialist hospital in Krakow. Investigations performed between 1995 and 2010 included the group of 5333 individuals. Dermatophyte infections, confirmed by culture, were revealed in 1007 subjects (18.9%), i.e. in 553 males and 454 females. The most frequent clinical forms of infections were tinea unguium and tinea pedis, caused mainly by Trichophyton rubrum and by Trichophyton mentagrophytes. Tinea corporis, tinea manuum, tinea capitis and tinea cruris constituted a small percentage of infections and the main aetiological factors of these dermatomycoses were also T. rubrum and T. mentagrophytes. Between 1995 and 2000 there were stated small differences in the number of isolated strains of dermatophytes in comparison with the number of examined patients. Since 2006 there has been observed a decrease in number of patients in our hospital with suspected fungal infections, but per cent of positive cultures has remained unchanged in comparison with earlier period. © 2013 Blackwell Verlag GmbH.

Koncelik D.L.,Microbiological Laboratory | Hernandez J.,Hospital Pharmacy
American Journal of Clinical Pathology | Year: 2016

Objectives: A study was conducted to evaluate the impact of implementing the Staphylococcus QuickFISH assay (AdvanDx,Woburn,MA), which rapidly detects and differentiates Staphylococcus aureus from coagulase-negative staphylococci (CoNS), together with an antimicrobial stewardship program on treating patients suspected of having sepsis. Methods: Two patient groups showing CoNS in positive blood cultures were evaluated by either conventional or QuickFISH testing with respect to turnaround time (TAT) for microorganism identification following Gram stain. Length of hospital stay (LOS) and days on the antibiotic vancomycin (DOV) were also compared. Results: QuickFISH identification test accuracy was 100% compared with conventional testing. Average values for TAT, LOS, and DOV were all decreased as the result of QuickFISH testing; for acute-care patients hospitalized for 10 days or less, the main population of interest for this study, these three measures were all reduced significantly following implementation of QuickFISH vs conventional testing (P .001, P =.0484, and P =.0084, respectively). Based on certain assumptions, QuickFISH testing also led to substantial cost savings. Conclusions: The QuickFISH assay, with its ability to provide timely and actionable results nearly simultaneously with the Gram stain, in conjunction with an effective antimicrobial stewardship program, has been adopted as standard of care at our community-based hospital. © American Society for Clinical Pathology, 2016. All rights reserved.

Bredl S.,Redbiotec | Bredl S.,University of Regensburg | Plentz A.,University of Regensburg | Wenzel J.J.,University of Regensburg | And 3 more authors.
Journal of Clinical Virology | Year: 2011

Background: Acute parvovirus B19 (B19V) infection is characterized by high-level viremia. Antibodies against the capsid proteins VP1 and VP2 may complex with B19V-particles thereby becoming undetectable in diagnostic tests. Objectives: We intended to obtain data on the frequency of false-negative serology in acute B19V-infection. Study design: 129 plasma or serum samples of healthy blood donors and of patients with suspected B19V-infection were analyzed for B19V-DNA by qPCR and VP1/VP2-specific IgG and IgM by ELISA. Eleven of these samples were derived from four pregnant women with previous contact to B19V-infected individuals. Using acidic conditions virus/antibody-complexes were disrupted and detected by WesternLine and ELISA. Results: 83/118 samples were derived from acutely infected individuals displaying viremia (10 3-10 12geq/mL). In 24/83 viremic samples (28.9%) VP1/VP2-specific IgM and IgG were undetectable in ELISA, but could be demonstrated to be complexed with B19V-particles. Each 7/83 (8.4%) was IgM-positive/IgG-negative and IgM-negative/IgG-positive, in 45/83 samples (54.2%) IgG and IgM could be detected. 35 samples did not contain B19V-DNA; five of these were from seronegative persons. Analyzing consecutive sera derived from four pregnant women, B19V-DNA was demonstrated in 10/11 samples, B19V-specific IgG- and IgM-antibodies were detectable in 10/11 and 4/11 samples, respectively. In 2/4 women seroconversion was observed, but IgM was not detected in 50% of the samples. B19V-specific IgG but not IgM was detectable in 2/4 women. Conclusion: Acute B19V-infection cannot be diagnosed by exclusive analysis of B19V-specific antibodies. Only the combination of assays for detection of B19V-DNA and antibodies enables correct serodiagnosis. © 2011 Elsevier B.V.

Srifeungfung S.,Mahidol University | Tribuddharat C.,Mahidol University | Comerungsee S.,Mahidol University | Chatsuwan T.,Chulalongkorn University | And 4 more authors.
Vaccine | Year: 2010

The serotype of 172 S. pneumoniae isolates obtained from normally sterile sites from January 2006 to February 2009 in Thai patients was evaluated. The most common serotypes were 6B, 23F, 14, 19F, and 19A in patients <5 year-old, and 6B, 19A, 23F, 4, 9V in patients >65-year old. Seven-valent pneumococcal conjugated vaccine (PCV-7) covered 70.3%, 43.6%, and 43.5% of patients <5, 5-64 and ≥65 years of age, respectively, while PCV-13 covered 81.2%, 59.7%, and 60.9%, respectively. PCV-9, PCV-10, PCV-11 had very similar coverage as PCV-7. The antibiotic susceptibility rates of the isolates from sterile sites were 88.7-95.7% for penicillin, 90.6-98.4% for cefotaxime, 92.2-100% for ofloxacin and 100% for ciprofloxacin. PCV-7 covered 83% and 100%, respectively, of penicillin and cefotaxime non-susceptible isolates in patients <5-year old. © 2010 Elsevier Ltd. All rights reserved.

Nowak P.,Jagiellonian University | Paluchowska P.,Jagiellonian University | Budak A.,Jagiellonian University | Budak A.,Microbiological Laboratory
New Microbiologica | Year: 2012

Acinetobacter baumannii is an important nosocomial pathogen occurring particularly in intensive care (ICU) as well as burn therapy units (BTU). A. baumannii strains have emerged as resistant to almost all antimicrobial agents, including carbapenems. β-lactamase-mediated resistance is the most common mechanism for carbapenem resistance in this species. Carbapenem-hydrolysing class D β-lactamases - OXA are widespread among A. baumannii strains. It is suggested that ISAba1 plays an important role in drug resistance. The aims of the study were detection of OXA encoding genes and presence of ISAba1. The study included the total of 104 isolates of carbapenem-resistant A. baumannii, obtained from patients hospitalized in ICU and BTU of Specialized Hospital in Krakow. Multiplex PCR was applied for detection of selected OXA carbapenemases encoding genes. PCR analysis showed the presence of blaOXA-51-like gene and ISAba1 in all isolates. 46 strains carried blaOXA-51-like and blaOXA-23-like genes while 48 blaOXA-51-like and blaOXA-40-like genes. 3 isolates carried: blaOXA-51-like, blaOXA-23-like and blaOXA-40-like genes. 7 strains encoded an OXA-51-like car-bapenemase but were negative for enzymes belonging to the other families tested. Comparative analysis of ICU and BTU isolates revealed the dominance of: blaOXA-51-like and blaOXA-40-like among ICU while blaOXA-51-like and blaOXA-23-like in BTU.

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