Microbe Russian Research Antiplague Institute

Saratov, Russia

Microbe Russian Research Antiplague Institute

Saratov, Russia
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Mikshis N.I.,Microbe Russian Research Antiplague Institute | Zhivova Yu.N.,Microbe Russian Research Antiplague Institute | Novikova L.V.,Microbe Russian Research Antiplague Institute | Sharapova N.A.,Microbe Russian Research Antiplague Institute | And 2 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2012

Comparative analysis of nucleotide sequences in genes that encode synthesis of methionine metabolism enzymes in the Bacillus anthracis strains and closely related bacterial species was conducted. A 42-bp deletion in the hom2 gene, which determines homoserine dehydrogenase, was found in all tested strains of the anthrax microbe. No mutation in the hom2 gene blocking the biosynthesis of methionine and threonine was registered in the strains of other bacillary species. Single nucleotide polymorphism was detected in the asd1, metX, and metH genes, which allows the B. anthracis strains to be identified by means of sequence tech- nology. © Allerton Press, Inc., 2012.


Mikshis N.I.,Microbe Russian Research Antiplague Institute | Kashtanova T.N.,Microbe Russian Research Antiplague Institute | Kutyrev V.V.,Microbe Russian Research Antiplague Institute
Molecular Genetics, Microbiology and Virology | Year: 2015

Nucleotide sequence analysis of several genes responsible for definitive properties of the anthrax pathogen—motility and penicillinase activity—determined a chromosomal locus promising for interspecies differentiation. We demonstrated that the fliC gene encoding flagellin synthesis contains an extended region distinguishing B. anthracis strains from the majority of nonpathogenic and opportunistic bacilli. A novel method for anthrax pathogen indication and identification based on determination of the differences in the fliC and hom2 chromosomal genes structure has been proposed. A total of 60 strains of different Bacillus spp. (B. anthracis, B. cereus, B. thuringiensis, B. mycoides, B. megaterium, B. subtilis, etc.) were tested using two chromosomal DNA targets. The algorithm developed in this work permits detection of the pathogenic microorganism and reliably differentiation of it from other Bacillus spp. representatives. The introduction of primers complementary to specific sequences of pXO1 and pXO2 plasmids into multiplex PCR makes it possible to obtain additional information on the proposed virulence of the isolate. © 2015, Allerton Press, Inc.

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