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Kuznetsova E.M.,Microbe Russian Antiplague Research Institute | Volokh O.A.,Microbe Russian Antiplague Research Institute | Shepelev I.A.,Microbe Russian Antiplague Research Institute | Nikiforov A.K.,Microbe Russian Antiplague Research Institute
Molecular Genetics, Microbiology and Virology | Year: 2012

The study presents the structural characteristics of the Francisella tularensis protective antigen complex (PAC)-a water-soluble antigen of the outer membrane (OMs) of the tularemia microorganism with a complex chemical nature with molecular mass of about 280 kDa. The immunochemically active subunits of the antigen protective complex with molecular masses from 81 to 19 kDa are established as having a protein nature, and those with 14-17 kDa are of lipoprotein nature. Stress chaperones (GroES and GroEL), the OM proteins (FRL-0617 and OmpH), regulatory proteins (EF-Tu and RplL), and bacterial enzymes (KatG and GAPDH) and a lipopolysaccharide were indentified in the PAC, which are responsible for its high immuno-biological activity. © Allerton Press, Inc., 2012.

Odinokov G.N.,Microbe Russian Antiplague Research Institute | Eroshenko G.A.,Microbe Russian Antiplague Research Institute | Krasnov J.M.,Microbe Russian Antiplague Research Institute | Guseva N.P.,Microbe Russian Antiplague Research Institute | Kutyrev V.V.,Microbe Russian Antiplague Research Institute
Russian Journal of Genetics | Year: 2011

The nucleotide sequences of the Tc's insect toxin complex genes have been analyzed in 18 natural strains of the main and non-main subspecies of Yersinia pestis isolated in different natural foci in the Russian Federation, as well as neighboring and more remote countries, as compared to the data on Y. pestis and Y. pseudotuberculosis strains stored in the NCBI GenBank database. The nucleotide sequences of these genes in plague agent strains have been found to be highly conserved, in contrast to those of the pseudotuberculosis agent. The sequences of two genes, tcaC and tccC2, have been found to be almost identical in Y. pestis strains, whereas other three genes (tcaA, tcaB, and tccC1) contain a few mutations, which, however, are not common for all strains of the plague agent. Exceptions are only strains of the Y. pestis biovar orientalis, whose tcaB gene is in a nonfunctional state due to a nucleotide deletion. The results suggest that the formation of the species Y. pestis as an agent of a natural focal infection with a transmissive mechanism has not resulted in degradation of the Tc's complex genes. Instead, these genes are likely to have been altered as the plague agent have been adapting to the new environment. © 2011 Pleiades Publishing, Ltd.

Mikshis N.I.,Microbe Russian Antiplague Research Institute | Kudryavtseva O.M.,Microbe Russian Antiplague Research Institute | Shulepov D.V.,Microbe Russian Antiplague Research Institute | Goncharova A.Y.,Microbe Russian Antiplague Research Institute | And 4 more authors.
Applied Biochemistry and Microbiology | Year: 2011

An asporogenic recombinant strain Bacillus anthracis 55ΔTPA-1(Spo -) producing anthrax protective antigen (PA) was obtained. The strain contains structural gene pag as a part of a hybrid replicon pUB110PA-1 and lacks determinants encoding the synthesis of main factors of anthrax pathogenicity. The level of PA production by asporogenic genetically engineered strain is approximately 80 μg/ml that is 4-5 times more than the values determined for vaccine strains B. anthracis STI-1 and B. anthracis 55. The strain preserves asporogenicity and ability to replicate the hybrid plasmid after in vitro passages. Biologically active PA was isolated from the constructed strain B. anthracis 55ΔTPA-1(Spo -). Double immunization of rabbits with 50 μg of the purified recombinant product provides their 100% protection from infection with 50 LD 50 of a highly virulent anthrax strain. © 2011 Pleiades Publishing, Ltd.

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