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Smirnova N.I.,Microbe Russian Anti Plague Research Institute | Goryaev A.A.,Microbe Russian Anti Plague Research Institute | Kutyrev V.V.,Microbe Russian Anti Plague Research Institute
Molecular Genetics, Microbiology and Virology | Year: 2010

A review of the modern data concerning the phenotypical and genetic peculiarities of the recently emerged highly virulent variants of V. cholerae El-Tor biovar (the etiological agent of the current cholera pan-demic) is presented. The molecular-genetic mechanisms that are considered to be a basis for V. cholerae genome evolution during the modern period are discussed. The transposon role in the increased expression of cholera toxin genes, which are constituents of the CTXφ prophage genome, is addressed. The need to work out new gene-diagnostic test-systems for the identification of natural altered variants of the cholera etiolog-ical agent is shown. © Allerton Press, Inc., 2010. Source

Odinokov G.N.,Microbe Russian Anti Plague Research Institute | Eroshenko G.A.,Microbe Russian Anti Plague Research Institute | Kookleva L.M.,Microbe Russian Anti Plague Research Institute | Shavina N.Y.,Microbe Russian Anti Plague Research Institute | And 2 more authors.
Russian Journal of Genetics | Year: 2012

Based on the results of computer analysis of nucleotide sequences in strains Yersinia pestis and Y. pseudotuberculosis recorded in the files of NCBI GenBank database, differences between genes argA, aroG aroF thiH, and thiG of strain Pestoides F (subspecies caucasica) were found, compared to other strains of plaque agent and pseudotuberculosis microbe. Using PCR with calculated primers and the method of sequence analysis, the structure of variable regions of these genes was studied in 96 natural Y. pestis and Y. pseudotuberculosis strains. It was shown that all examined strains of subspecies caucasica, unlike strains of plague-causing agent of other subspecies and pseudotubercolosis microbe, had identical mutations in genes argA (integration of the insertion sequence IS100), aroG (insertion of ten nucleotides), aroF (inserion of IS100), thiH (insertion of nucleotide T), and thiG (deletion of 13 nucleotides). These mutations are the reason for the absence in strains belonging to this subspecies of the ability to synthesize arginine, phenylalanine, tyrosine, and vitamin B1 (thiamine), and cause their auxotrophy for these growth factors. © 2012 Pleiades Publishing, Ltd. Source

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