Gifu-shi, Japan
Gifu-shi, Japan

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Yabuta Y.,Agricultural | Hashimoto E.,Agricultural | Takeuchi T.,Tottori University | Sakaki S.,MicroAlgae Corporation | And 3 more authors.
Food Science and Technology Research | Year: 2014

Cyanobacteria contain substances of high biological value and are used to meet the high demands of the global food and pharmaceutical industries. In this study, we prepared a hot water extract of the edible cyanobacterium, Nostochopsis, and evaluated its possible use as an ingredient in cosmetics. Specifically, a hot water extract of Nostochopsis was assayed for tyrosinase inhibitory activity. Tyrosinase, a key enzyme in skin melanin synthesis, was strongly inhibited by the Nostochopsis hot water extract, which reduced a-melanocyte-stimulating hormone-induced melanin synthesis in B16 mouse melanoma cells. Thus, Nostochopsis hot water extract may be suitable as an ingredient in cosmetics. Copyright © 2014, Japanese Society for Food Science and Technology.

Itoh T.,Kinki University | Tsuzuki R.,Kinki University | Tanaka T.,Kinki University | Ninomiya M.,Gifu University | And 5 more authors.
Food and Chemical Toxicology | Year: 2013

Nostoc commune is a terrestrial benthic blue-green alga that often forms an extended mucilaginous layer on the soil, accumulates on stones and mud in aquatic environments. Reduced-scytonemin (R-scy), isolated from N. commune Vaucher, has been shown to suppress the human T-lymphoid Jurkat cell growth. To reveal the mechanisms underlying the R-scy-mediated inhibition of Jurkat cell growth, we examined cell morphology, DNA fragmentation, and microtubule-associated protein light chain 3 (LC3) modification in these cells. We observed multiple vacuoles as well as the conversion of LC3-I to LC3-II in R-scy-treated cells. These results suggest that the R-scy induced Jurkat cell growth inhibition is attributable to the induction of type II programmed cell death (PCD II; autophagic cell death or autophagy). We further examined the mechanisms underlying R-scy-induced PCDII. The cells treated with R-scy produced large amounts of reactive oxygen species (ROS), leading to the induction of mitochondrial dysfunction. However, the elimination of R-scy-induced ROS by treatment with N-acetyl-. l-cysteine (NAC) markedly opposed R-scy-induced PCDII. Based on these results, we conclude that ROS formation plays a critical role in R-scy-induced PCDII. © 2013 Elsevier Ltd.

Hashimoto E.,Tottori University | Yabuta Y.,Tottori University | Takenaka S.,Osaka Prefecture University | Yamaguchi Y.,MicroAlgae Corporation | And 2 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2012

Vitamin B12 content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6±16.2 μg/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B12 or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B12 (97.4±11.8 μg/100 g dry weight) and vitamin B12 (43.2±6.0 μg/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B12 content was significantly increased in the Nostochopsis cells (254.8±17.6 μg/100 g dry weight) grown in the vitamin B12-supplemented medium.

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