Micro and Nanofabrication Unit

Eibar, Spain

Micro and Nanofabrication Unit

Eibar, Spain
SEARCH FILTERS
Time filter
Source Type

Baran E.T.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Baran E.T.,ICVS 3Bs PT Government Associated Laboratory | Pirraco R.P.,European Institute of Excellence on Tissue Engineering and Regenerative Medicine | Pirraco R.P.,ICVS 3Bs PT Government Associated Laboratory | And 10 more authors.
Journal of Bioactive and Compatible Polymers | Year: 2015

In this study, cell responses on micropatterned films that were changing in groove-ridge widths and pattern depth were investigated to compare the degree of size effects from X-Y and Z planes. Poly(caprolactone) films with five different groove-ridge sizes and three pattern depths were prepared by hot embossing technique. In general, the morphologies of osteoblast cell were not changed noticeably by the size changes in groove-ridges with the same depth size. However, cell morphologies were changed significantly when pattern depths were increased from 1.35 to 4.95‰μm. Also, the cell morphology change between different groove-ridges was significant when the pattern depth was small (1.35‰μm), and these effects were diminished or masked when the pattern depth was increased to 4.95‰μm. Linear regression analysis further clarifies that unit size changes in depth may affect cell length and orientation rates 2.4 and 4 times, respectively, in comparison to rates obtained from X-Y planes. © SAGE Publications.


Martinez-Perdiguero J.,CIC microGUNE | Retolaza A.,CIC microGUNE | Retolaza A.,Micro and Nanofabrication Unit | Otaduy D.,CIC microGUNE | And 5 more authors.
Sensors (Switzerland) | Year: 2013

In this work we present a surface plasmon resonance sensor based on enhanced optical transmission through sub-wavelength nanohole arrays. This technique is extremely sensitive to changes in the refractive index of the surrounding medium which result in a modulation of the transmitted light. The periodic gold nanohole array sensors were fabricated by high-throughput thermal nanoimprint lithography. Square periodic arrays with sub-wavelength hole diameters were obtained and characterized. Using solutions with known refractive index, the array sensitivities were obtained. Finally, protein absorption was monitored in real-time demonstrating the label-free biosensing capabilities of the fabricated devices. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Postigo P.A.,Imm Institute Microelectronica Of Madrid | Alvaro R.,Imm Institute Microelectronica Of Madrid | Juarros A.,Micro and Nanofabrication Unit | Merino S.,Micro and Nanofabrication Unit
Biomedical Optics Express | Year: 2016

The fabrication of a novel optofluidic chip using nanochannels optimized for DNA-stretched molecules and optical detection by enhanced fluorescence is reported. The chips are composed of a series of microchannels that allow the transport of molecules in the femto-liter per second inside a fluid or gas. The nanochannels are surrounded by a photonic crystal structure to enhance the emission of fluorescent light from the molecules, which can travel along the nanochannel, allowing for enhanced optical detection of the molecules in motion. The photonic crystal structure provides an enhancement up to 2.5 times in the light emitted from fluorescent molecules inside the nanochannels which increases to around 250 when normalized to the area of the nanochannels emitting fluorescence. The results may help to the detection of fluorescent molecules (like marked-DNA) in series by speeding it and allowing the use of less sophisticated equipment. © 2016 Optical Society of America.


PubMed | Micro and Nanofabrication Unit and Imm Institute Microelectronica Of Madrid
Type: Journal Article | Journal: Biomedical optics express | Year: 2016

The fabrication of a novel optofluidic chip using nanochannels optimized for DNA-stretched molecules and optical detection by enhanced fluorescence is reported. The chips are composed of a series of microchannels that allow the transport of molecules in the femto-liter per second inside a fluid or gas. The nanochannels are surrounded by a photonic crystal structure to enhance the emission of fluorescent light from the molecules, which can travel along the nanochannel, allowing for enhanced optical detection of the molecules in motion. The photonic crystal structure provides an enhancement up to 2.5 times in the light emitted from fluorescent molecules inside the nanochannels which increases to around 250 when normalized to the area of the nanochannels emitting fluorescence. The results may help to the detection of fluorescent molecules (like marked-DNA) in series by speeding it and allowing the use of less sophisticated equipment.

Loading Micro and Nanofabrication Unit collaborators
Loading Micro and Nanofabrication Unit collaborators