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Wu Y.-C.,National Taiwan University | Wu Y.-C.,Taichung District Agricultural Research and Extension Station | Chang S.-J.,Miaoli District Agricultural Research and Extension Station | Lur H.-S.,National Taiwan University
Plant Production Science | Year: 2016

Typical japonica type rice is sensitive to high temperature. Pon-Lai rice is a special japonica type with adaptation to the subtropical climate in Taiwan. Facing climate change, rising temperatures would damage the yield and quality of rice production. This research was conducted using Pon-Lai rice in the field of a subtropical climate. We conducted 2 experiments, including a year-round experiment and collection of samples from different districts for building different temperature conditions. We analyzed the correlation between rising temperature and rice yield or quality. In our results, the critical period of temperature effect is 0–15 days after heading (H15). The threshold of high temperature damage in yield and appearance quality was 25–27 °C. Grain weight decreased about 2–6%, while the temperature of H15 was raised 1 °C above the thresholds. Perfect grain ratio and chalky grain ratio decreased and increased, respectively, while the temperature of H15 was raised above the thresholds. However, the high temperature in H15 affected the physicochemical characteristics. In addition, we found positive correlation between grain length to width ratio and perfect grain ratio. Grain length to width ratio could be an index of temperature effects for grain quality. In our study, when the temperature was below 30 °C, a rising temperature of H15 could damage rice yield and appearance quality, and change grain shape. Our results could provide reference for dealing with the warming future in other temperate rice-cultivated countries. © 2016 The Author(s). Source


Chen C.-C.,Miaoli District Agricultural Research and Extension Station | Huang W.-D.,National Taiwan University | Yang C.-M.,Academia Sinica, Taiwan
Research Journal of Biotechnology | Year: 2016

Our objectives in this study were to investigate morphological traits and dynamics of chlorophyll (Chl) degradation intermediates (chlorophyllide, Chlide; pheophytin, Phe; pheophorbide, Pho) in leaves of rice (Oryza sativa L.) seedlings under increasing green light intensity. Seedlings of Taichung Native 1 (TCN1) were grown under equal intensities (40 μmol m-2 s-1) of red and blue light with four levels of green light intensity (0, 20, 40 and 60 μmol m-2 s-1). Light emitting diodes (LED) were used to control lighting treatments. Sheaths of rice seedling leaves elongated and leaves grew erectly under red and blue light with increasing green light intensity. These morphological traits are known as shade avoidance symptoms (SAS). Increasing green light intensity resulted in decreases in total chlorophyll, the effective quantum yield of photosystem II (ΦPSII) and Phe/Chlide ratios and increases in non-photochemical quenching (NPQ) and Chlide levels. These results indicated that green light induced SAS and mediated Chl degradation routes in rice seedlings. Source


Lu M.-C.,Miaoli District Agricultural Research and Extension Station
Acta Horticulturae | Year: 2010

Pleione formosana Hayata, known as the one-foliate orchid, is a native terrestrial orchid in Taiwan. This study investigated the effect of mother bulb size on flowering and daughter bulb formation, and the application of gibberellic acid (GA) on the bulb growth in Nan-Chung village. The results showed that the bulb size was in proportion to the flowering percentage, floret number, diameter, scape number and length. However, the shelf life was not influenced by bulb size. The number and weight of daughter bulbs after harvest was in proportion to the size of mother bulbs. Different concentrations of GA 3 (0, 25, 50, 75, 125 mg/L) was applied to bulbs by spraying during growth or immersion before planting, and the daughter bulb characteristics were investigated after harvest. The results indicated that GA 3 spraying during growth significantly increased the total fresh weight, diameter and height of daughter bulbs. Source


Lu M.-C.,Miaoli District Agricultural Research and Extension Station
Acta Horticulturae | Year: 2010

An in vitro method was developed to regenerate plantlets from protocormderived callus of Geodorum densiflorum (Lam.) Schltr. cultured on half-strength Murashige-Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) at 3 to 5 mg/L and 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (TDZ) at 0.1 to 0.5 mg/L in the dark. The proliferation rate of the pale-yellow embryogenic callus was 3.9 to 4.2 fold in mediums containing 2,4-D 3 mg/L and TDZ 0.1 to 0.5 mg/L after one month of culture in the dark. The callus was regenerated to plantlets via protocorm-like bodies (PLBs) on medium containing 0.5 mg/L TDZ after 6 months of culture, and 644 PLBs and 56 shoot buds per 0.1 g callus were obtained after 12 months of culture. The well-rooted plantlets with pseudobulbs were transferred to sphagnum-containing pots and acclimatized in a greenhouse. The embryogenic callus of the endangered orchid, Geodorum densiflorum has been established. Source


Wang R.-J.,Miaoli District Agricultural Research and Extension Station | Wang R.-J.,National Chung Hsing University | Hu M.-L.,National Chung Hsing University
International Journal of Food Properties | Year: 2011

This study provides a basis and principle for developing an integrated antioxidant assay of mulberry. Five genotypes (Miaoli No. 1, 73C020, 46C019, 74H3023, and 68H22024) and three maturity stages (unripe, medium ripe and fully ripe) of mulberry (Morus sp.) fruit were analyzed for their total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl-scavenging (DPPH-SC) ability, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). Significant correlations were obtained for the four assays used (r ranging from 0.390 to 0.787, all P < 0.01). Two-way ANOVA revealed that there were significant effects of genotype, maturity stage, and the interaction of genotypes and maturity stages for TPC, FRAP and ORAC, whereas only maturity stage and the interaction term were significant for DPPH-SC activity. Principal component analysis showed that Miaoli No. 1 and 74H 3023 possessed the highest antioxidant capacity, while genotype 68H22024 possessed the lowest. Principal component analysis could group and separate mulberry genotypes based on their different antioxidant activities. Overall, the present results provided basic data for choosing mulberry fruits with higher antioxidant activity for direct consumption or for production of fruit juice. Copyright © Taylor & Francis Group, LLC. Source

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