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Tan H.,Sichuan Agricultural University | Huang H.,Mianyang Institute of Agricultural Science | Tie M.,Dazhou Institute of Agricultural Science | Tang Y.,Sichuan Agricultural University | And 2 more authors.

Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars - "Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively - were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic engineering, and advance our knowledge of the genes involved in the complex regulatory networks of this plant under cold stress. © 2016 Tan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Ren Y.,Sichuan Agricultural University | Li S.,Mianyang Institute of Agricultural Science | Luo J.,Mianyang Institute of Agricultural Science | He Z.,Chinese Academy of Agricultural Sciences | And 5 more authors.
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji

The development and utilization of outstanding germplasm in breeding programs can expedite breeding process. The high yielding variety Mianmai 37, grown widely in southwestern China, has been used widely in breeding programs. Comparisons between Mianmai 37 and its derivatives for yield and yield components were conducted. Simple sequence repeat (SSR) markers were used to test the frequency of specific alleles transferred from Mianmai 37 to its derivative culti-var Mianmai 367. The results indicated that the yield of the derivative cultivars was significantly higher than Mianmai 37, due to an increased grain number per spike. Favorable traits from Mianmai 37 such as resistance to stripe rust, were trans-ferred to its derivatives. At molecular level, 78.9% loci in Mianmai 367 were derived from Mianmai 37 with 75.0, 83.6 and 74.2% from A, B and D genomes, respectively. Mianmai 367 shared common loci with its parent Mianmai 37, such as re-gions Xgwm374-Xbarc167-Xbarc128-Xgwm129-Xgwm388-Xbarc101 on chromosome 2B and Xwmc446-Xwmc366- Xwmc533-Xbarc164-Xwmc418 on chromosome 3B, these regions were associated with grain number, 1000-kernel weight and resistance. The preferred transmission of alleles from Mianmai 37 to its derivatives probably can be explained by the strong selection pressures because of its favorable agronomic traits and the disease resistance. Source

Ren Y.,Sichuan Agricultural University | Ren Y.,Mianyang Institute of Agricultural Science | Li S.-R.,Mianyang Institute of Agricultural Science | Wei Y.-M.,Sichuan Agricultural University | And 4 more authors.
Journal of Integrative Agriculture

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2:3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as YrMY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 cM, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and YrCH42 on chromosome 1B. Allelic tests indicated that YrMY41 is likely a new allele at Yr26 locus. © 2015 Chinese Academy of Agricultural Sciences. Source

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