Mianyang Academy of Agricultural science

Mianyang, China

Mianyang Academy of Agricultural science

Mianyang, China
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Zou X.,Mianyang Academy of Agricultural science | Zou X.,Sichuan Agricultural University | Deng M.-S.,Sichuan Agricultural University | Li L.-Q.,Sichuan Agricultural University | And 7 more authors.
Acta Agronomica Sinica | Year: 2017

To explore the role of brassinosteroid (BR) in tuber sprouting and establish effective sprouting regulation system for seed potato, three varieties and sprouting-inhibitor treatments were used to analyze expression characteristics of nine genes related to BR synthesis, signal transduction and regulation during storage. Effects of BR analogue 24-epibrassinolide (24-eBL) and gibberellin (GA3) on tuber sprouting were also studied. The transcript levels of four genes involved in BR synthesis increased with prolonging storage time, and the time points at which the expression levels began to increase in variety with short dormancy period were earlier than those in varieties with middle or long dormancy period. The expression patterns of BRI1 and CYCD3 were similar to those of synthetic genes while BSK and TCH4 remained constant in varieties with middle or long dormancy period. Sprouting-inhibitors stimulated transcripts of those genes to elevate in the earlier stage of storage, then rapidly decline and stay low levels. There was no significant expression change of transcription factor BZR1 in varieties and sprouting-inhibitor treatments during storage. BR analogue 24-eBL was favorable to tuber dormancy release but shoot growth. The tuber weight per plant treated with 24-eBL and GA3 mixture increased by 37.92% to 98.41% compared with CK. The conclusion, BR synthesis and its signal transduction are essential physiological processes from dormancy to sprouting in potato tuber. Mixture of 24-eBL and GA3 can facilitate uniformity of seed potato sprouting, stronger growth and better tuberization.


Zhao Z.-X.,Sichuan Agricultural University | Zhao S.-L.,Sichuan Agricultural University | Shi J.,Sichuan Agricultural University | Shi J.,Mianyang Academy of Agricultural science | And 4 more authors.
Journal of Plant Pathology | Year: 2017

Rice blast is one of the most destructive diseases of rice worldwide. Three-line hybrid rice provides a high efficacy system to exploit heterosis that contributes to the yield increase in the past decades. A disease-resistant breeding program has released two elite male sterile lines, Gang Xiang 1A (GX1A) and D Xiang 4A (DX4A), and an elite restorer line, Shuhui 707 (SH707), which showed resistance to 102 isolates of Magnaporthe oryzae. To address the question whether the sterile lines and the restorer line contained different resistance genes, expression levels of the functionally cloned blast resistance genes were examined by real-time PCR (RT-PCR). Our data demonstrated that Pikm, Pia, Pid2, Pi-ta, and Pi5 were the candidate contributors of resistance in GX1A, DX4A and/or SH707, because of their high expression. Resistant spectrum assay and sequence polymorphism analysis narrowed down the potentially resistant contributors to Pikm and Pid2 in GX1A and DX4A, and Pi-ta, Pi5 and Pid2 in SH707, respectively. These data indicate that the male sterile lines and the restorer line possess different resistance genes and are highly valuable in disease-resistant breeding programs for developing new male sterile and restorer lines. Therefore, hybrid rice has the advantage to pyramid multiple disease resistance genes by introduction of different resistance genes into the parental male sterile lines and restorer lines. © 2017, Edizioni ETS. All rights reserved.


Xiong B.,Sichuan Agricultural University | Gu X.,Mianyang Academy of Agricultural science | Qiu X.,Sichuan Agricultural University | Dong Z.,Sichuan Agricultural University | And 6 more authors.
PLoS ONE | Year: 2017

Considering the known effects of xyloglucan endotransglycosylase (XET) on plant growth and development, we aimed to determine whether XETs help to regulate the growth and elongation of Huangguogan shoots and roots. We confirmed a possible role for XET during seedling etiolation. Our results revealed that the roots of etiolated seedlings (H-E) ere longer than those of green seedlings (H-G). However, shoot length exhibited the opposite pattern. We also observed positive and negative effects on the xyloglucan-degrading activity of XET in the root sub-apical region and shoots of etiolated Huangguogan seedling, respectively. There was a significant down-regulation in CitXET expression in the etiolated shoots at 15 days after seed germination. On the contrary, it was significantly increased in the root sub-apical region of etiolated and multicolored seedlings at 15 days after seed germination. The XET coding sequence (i.e., CitXET) was cloned from Huangguogan seedlings using gene-specific primers. The encoded amino acid sequence was predicted by using bioinformatics-based methods. The 990-bp CitXET gene was highly homologous to other XET genes. The CitXET protein was predicted to contain 319 amino acids, with a molecular mass of 37.45 kDa and an isoelectric point of 9.05. The predicted molecular formula was C1724H2548N448O466S14, and the resulting protein included only one transmembrane structure. The CitXET secondary structure consisted of four main structures (i.e., 21% α-helix, 30.72% extended strand, 9.09% β-turn, and 39.18% random coil). Analyses involving the NCBI Conserved Domains Database (NCBI-CDD), InterPro, and ScanProsite revealed that CitXET was a member of the glycosyl hydrolase family 16 (GH16), and included the DEIDFEFLG motif. Our results indicate that the differed degrees of etiolation influenced the CitXET expression pattern and XET activity in Huangguogan seedlings. The differential changes in XET activity and CitXET expression levels in Huangguogan seedlings may influence the regulation of root and shoot development, and may be important for seedling etiolation. © 2017 Xiong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Zhang X.,Sichuan Agricultural University | Zhang X.,Key laboratory of Biology and Genetic Improvement of Maize in Southwest Region | Zhang H.,Sichuan Agricultural University | Zhang H.,Mianyang Academy of Agricultural science | And 18 more authors.
BMC Genomics | Year: 2016

Background: Maize breeding germplasm used in Southwest China has high complexity because of the diverse ecological features of this area. In this study, the population structure, genetic diversity, and linkage disequilibrium decay distance of 362 important inbred lines collected from the breeding program of Southwest China were characterized using the MaizeSNP50 BeadChip with 56,110 single nucleotide polymorphisms (SNPs). Results: With respect to population structure, two (Tropical and Temperate), three (Tropical, Stiff Stalk and non-Stiff Stalk), four [Tropical, group A germplasm derived from modern U.S. hybrids (PA), group B germplasm derived from modern U.S. hybrids (PB) and Reid] and six (Tropical, PB, Reid, Iowa Stiff Stalk Synthetic, PA and North) subgroups were identified. With increasing K value, the Temperate group showed pronounced hierarchical structure with division into further subgroups. The Genetic Diversity of each group was also estimated, and the Tropical group was more diverse than the Temperate group. Seven low-genetic-diversity and one high-genetic-diversity regions were collectively identified in the Temperate, Tropical groups, and the entire panel. SNPs with significant variation in allele frequency between the Tropical and Temperate groups were also evaluated. Among them, a region located at 130 Mb on Chromosome 2 showed the highest genetic diversity, including both number of SNPs with significant variation and the ratio of significant SNPs to total SNPs. Linkage disequilibrium decay distance in the Temperate group was greater (2.5-3 Mb) than that in the entire panel (0.5-0.75 Mb) and the Tropical group (0.25-0.5 Mb). A large region at 30-120 Mb of Chromosome 7 was concluded to be a region conserved during the breeding process by comparison between S37, which was considered a representative tropical line in Southwest China, and its 30 most similar derived lines. Conclusions: For the panel covered most of widely used inbred lines in Southwest China, this work representatively not only illustrates the foundation and evolution trend of maize breeding resource as a theoretical reference for the improvement of heterosis, but also provides plenty of information for genetic researches such as genome-wide association study and marker-assisted selection in the future. © 2016 The Author(s).


Lin L.,Sichuan Agricultural University | Liu Q.,Shandong Academy of Agricultural Sciences | Shi J.,Mianyang Academy of Agricultural science | Sun J.,Sichuan Agricultural University | And 2 more authors.
Environmental Toxicology and Chemistry | Year: 2014

Genetic diversity has supplied effective ways to improve crop yields and disease resistance. Therefore, crop uptake of heavy metals may be reduced by collecting germplasm resources. In the present study, cadmium accumulation and nutrients in radish were investigated by intercropping 3 genotypes (red, green, and white radish) in different combinations. Both pot and field experiments showed that cadmium content in radish was increased by intercropping 2 or 3 genotypes, except when white radish was intercropped with green radish. The biomass of red radish was improved by a mixture of all 3 genotypes, green radish biomass was improved by intercropping with the other 2 varieties, and white radish biomass was improved by intercropping with red radish in both pot and field experiments. The pot experiment indicated that the soil exchangeable cadmium concentration of red radish grown with green radish was lower than that of monoculture, whereas red radish intercropped with white radish was between the respective monocultures; the soil exchangeable cadmium concentrations of green radish grown with white radish and with all 3 genotypes grown together were greater than those of the monocultures. Some intercropping modes also improved potassium and phosphorus contents in the edible parts of radish in the pot experiment. © 2014 SETAC.


Lin L.,Sichuan Agricultural University | Shi J.,Mianyang Academy of Agricultural science | Liu Q.,Shandong Academy of Agricultural Sciences | Liao M.,Sichuan Agricultural University | Mei L.,Sichuan Agricultural University
Environmental Monitoring and Assessment | Year: 2014

In a preliminary study, we found that the cadmium (Cd) concentrations in shoots of the winter farmland weeds Cardamine hirsuta Linn. and Gnaphalium affine D. Don exceeded the critical value of a Cd-hyperaccumulator (100 mg kg-1), indicating that these two farmland weeds might be Cd-hyperaccumulators. In this study, we grew these species in soil containing various concentrations of Cd to further evaluate their Cd accumulation characteristics. The biomasses of C. hirsuta and G. affine decreased with increasing Cd concentrations in the soil, while the root/shoot ratio and the Cd concentrations in shoot tissues increased. The Cd concentrations in shoots of C. hirsuta and G. affine reached 121.96 and 143.91 mg kg-1, respectively, at the soil Cd concentration of 50 mg kg-1. Both of these concentrations exceeded the critical value of a Cd-hyperaccumulator (100 mg kg-1). The shoot bioconcentration factors of C. hirsuta and G. affine were greater than 1. The translocation factor of C. hirsuta was less than 1 and that of G. affine was greater than 1. These findings indicated that C. hirsuta is a Cd-accumulator and G. affine is Cd-hyperaccumulator. Both plants are distributed widely in the field, and they could be used to remediate Cd-contaminated farmland soil in winter. © 2014 Springer International Publishing.


Tang Y.-L.,Sichuan Academy of Agricultural science | Wu Y.-Q.,Sichuan Agricultural University | Zhu H.-Z.,Sichuan Academy of Agricultural science | Li C.-S.,Sichuan Academy of Agricultural science | And 4 more authors.
Acta Agronomica Sinica | Year: 2010

Sichuan Province has the largest wheat (Triticum aestivum L.) producing area in Southwest China. It is crucial to understand the quality potential and stability for the major commercial cultivars and to provide guidance to farmers regarding wheat production in this province. Seven representative cultivars were planted in 5 ecological localities of Sichuan under two nitrogen rates from 2006 to 2008. Eight quality traits and 2 end-use production (noodle and bread making) scores were determined. The average of 3-year test weight, grain protein content, wet gluten content, Zeleny sedimentation, falling number, flour water abstraction, dough developing time, stability time, noodle score of dry white Chinese noodle, and bread score were 777 g L-1, 12.3%, 25.1%, 32.9 mL, 326 s, 56.5%, 3.0 min, 4.5 min, 78.5, and 62.2, respectively. In a combined analysis of variance, the effects of genotype, environment, and their interaction were significant for almost all traits tested. For test weight, Zeleny sedimentation, falling number, flour water abstraction, and noodle score of dry white Chinese noodle, the influences between years were greater than differences between locations; whereas, for protein content, wet gluten content, dough developing time, stability time, and bread score, location had a greater influence than year. Increasing nitrogen rate significantly improved most quality traits and raised bread score, but had no influence on noodle score. Stability analysis revealed that Chuanmai 39 was stable for bread quality with the highest bread score value, while Chuanmai 37 was stable for most quality traits with the highest noodle score. This result indicates that Chuanmai 39 and Chuanmai 37 can be favorably used in wheat quality improvement in Sichuan Province. © 2010 Crop Science Society of China and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences.


Shi J.,Sichuan Agricultural University | Shi J.,Mianyang Academy of Agricultural science | Li D.,Sichuan Agricultural University | Li Y.,Sichuan Agricultural University | And 10 more authors.
Genome | Year: 2015

Rice blast, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most serious rice diseases worldwide. We previously developed an elite hybrid rice restorer line with high resistance to rice blast, Yahui2115 (YH2115). To identify the blast resistance genes in YH2115, we first performed expression profiling on previously reported blast resistance genes and disease assay on monogenic lines, and we found that Pi2, Pi9, and Pikm were the most likely resistance candidates in YH2115. Furthermore, RNA interference and linkage analysis demonstrated that silencing of Pi2 reduced the blast resistance of YH2115 and a Pi2 linkage marker was closely associated with blast resistance in an F2 population generated from YH2115. These data suggest that the broad-spectrum blast resistance gene Pi2 contributes greatly to the blast resistance of YH2115. Thus, YH2115 could be used as a new germplasm to facilitate rice blast resistance breeding in hybrid rice breeding programs. © 2015 Published by NRC Research Press.


Ren Y.,Mianyang Academy of Agricultural science
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important limiting factor to popularize hybrid wheat. The objectives of this study were to map a stripe rust resistance gene in a Chinese thermo-photo-sensitive hybrid wheat restore line MR168 using gene postulation and SSR markers. MR168 was highly resistant to 23 Pst races including CYR29, CYR31, CYR32, and CYR33. The populations F1, BC1, F2, and F3 from the cross between MR168 and SY95-71 (a wheat cultivar susceptible to Pst races) were inoculated with the race of Pst CYR32 of China in greenhouse. MR168 carried a single dominant gene for resistance to CYR32, tentatively designated YrMR168. It originated from Liaochun 10, a spring wheat variety. A total of 183 F2 plants, the resistant and susceptible parents and resistant and susceptible bulks were used for resistance gene mapping with 329 pairs of wheat SSR markers.Five SSR markers on chromosome 1BS including Xgwm18, Xbarc187, Xwmc269, Xgwm273, and Xwmc406 were linked with YrMR168. The resistance gene was closely linked to Xgwm18 and Xbarc187 with the genetic distances of 1.9 and 2.4 cM, respectively. Xgwm18 and Xbarc187 could be used for molecular marker assisted selection of YrMR168 in hybrid wheat breeding program.


PubMed | Mianyang Academy of Agricultural science
Type: Journal Article | Journal: Yi chuan = Hereditas | Year: 2011

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important limiting factor to popularize hybrid wheat. The objectives of this study were to map a stripe rust resistance gene in a Chinese thermo-photo-sensitive hybrid wheat restore line MR168 using gene postulation and SSR markers. MR168 was highly resistant to 23 Pst races including CYR29, CYR31, CYR32, and CYR33. The populations F1, BC1, F2, and F3 from the cross between MR168 and SY95-71 (a wheat cultivar susceptible to Pst races) were inoculated with the race of Pst CYR32 of China in greenhouse. MR168 carried a single dominant gene for resistance to CYR32, tentatively designated YrMR168. It originated from Liaochun 10, a spring wheat variety. A total of 183 F2 plants, the resistant and susceptible parents and resistant and susceptible bulks were used for resistance gene mapping with 329 pairs of wheat SSR markers.Five SSR markers on chromosome 1BS including Xgwm18, Xbarc187, Xwmc269, Xgwm273, and Xwmc406 were linked with YrMR168. The resistance gene was closely linked to Xgwm18 and Xbarc187 with the genetic distances of 1.9 and 2.4 cM, respectively. Xgwm18 and Xbarc187 could be used for molecular marker assisted selection of YrMR168 in hybrid wheat breeding program.

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