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Shi J.,Sichuan Agricultural University | Shi J.,Mianyang Academy of Agricultural science | Li D.,Sichuan Agricultural University | Li Y.,Sichuan Agricultural University | And 10 more authors.
Genome | Year: 2015

Rice blast, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most serious rice diseases worldwide. We previously developed an elite hybrid rice restorer line with high resistance to rice blast, Yahui2115 (YH2115). To identify the blast resistance genes in YH2115, we first performed expression profiling on previously reported blast resistance genes and disease assay on monogenic lines, and we found that Pi2, Pi9, and Pikm were the most likely resistance candidates in YH2115. Furthermore, RNA interference and linkage analysis demonstrated that silencing of Pi2 reduced the blast resistance of YH2115 and a Pi2 linkage marker was closely associated with blast resistance in an F2 population generated from YH2115. These data suggest that the broad-spectrum blast resistance gene Pi2 contributes greatly to the blast resistance of YH2115. Thus, YH2115 could be used as a new germplasm to facilitate rice blast resistance breeding in hybrid rice breeding programs. © 2015 Published by NRC Research Press. Source

Ren Y.,Mianyang Academy of Agricultural science
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important limiting factor to popularize hybrid wheat. The objectives of this study were to map a stripe rust resistance gene in a Chinese thermo-photo-sensitive hybrid wheat restore line MR168 using gene postulation and SSR markers. MR168 was highly resistant to 23 Pst races including CYR29, CYR31, CYR32, and CYR33. The populations F1, BC1, F2, and F3 from the cross between MR168 and SY95-71 (a wheat cultivar susceptible to Pst races) were inoculated with the race of Pst CYR32 of China in greenhouse. MR168 carried a single dominant gene for resistance to CYR32, tentatively designated YrMR168. It originated from Liaochun 10, a spring wheat variety. A total of 183 F2 plants, the resistant and susceptible parents and resistant and susceptible bulks were used for resistance gene mapping with 329 pairs of wheat SSR markers.Five SSR markers on chromosome 1BS including Xgwm18, Xbarc187, Xwmc269, Xgwm273, and Xwmc406 were linked with YrMR168. The resistance gene was closely linked to Xgwm18 and Xbarc187 with the genetic distances of 1.9 and 2.4 cM, respectively. Xgwm18 and Xbarc187 could be used for molecular marker assisted selection of YrMR168 in hybrid wheat breeding program. Source

Lin L.,Sichuan Agricultural University | Liu Q.,Shandong Academy of Agricultural Sciences | Shi J.,Mianyang Academy of Agricultural science | Sun J.,Sichuan Agricultural University | And 2 more authors.
Environmental Toxicology and Chemistry | Year: 2014

Genetic diversity has supplied effective ways to improve crop yields and disease resistance. Therefore, crop uptake of heavy metals may be reduced by collecting germplasm resources. In the present study, cadmium accumulation and nutrients in radish were investigated by intercropping 3 genotypes (red, green, and white radish) in different combinations. Both pot and field experiments showed that cadmium content in radish was increased by intercropping 2 or 3 genotypes, except when white radish was intercropped with green radish. The biomass of red radish was improved by a mixture of all 3 genotypes, green radish biomass was improved by intercropping with the other 2 varieties, and white radish biomass was improved by intercropping with red radish in both pot and field experiments. The pot experiment indicated that the soil exchangeable cadmium concentration of red radish grown with green radish was lower than that of monoculture, whereas red radish intercropped with white radish was between the respective monocultures; the soil exchangeable cadmium concentrations of green radish grown with white radish and with all 3 genotypes grown together were greater than those of the monocultures. Some intercropping modes also improved potassium and phosphorus contents in the edible parts of radish in the pot experiment. © 2014 SETAC. Source

Tang Y.-L.,Sichuan Academy of Agricultural science | Wu Y.-Q.,Sichuan Agricultural University | Zhu H.-Z.,Sichuan Academy of Agricultural science | Li C.-S.,Sichuan Academy of Agricultural science | And 4 more authors.
Acta Agronomica Sinica | Year: 2010

Sichuan Province has the largest wheat (Triticum aestivum L.) producing area in Southwest China. It is crucial to understand the quality potential and stability for the major commercial cultivars and to provide guidance to farmers regarding wheat production in this province. Seven representative cultivars were planted in 5 ecological localities of Sichuan under two nitrogen rates from 2006 to 2008. Eight quality traits and 2 end-use production (noodle and bread making) scores were determined. The average of 3-year test weight, grain protein content, wet gluten content, Zeleny sedimentation, falling number, flour water abstraction, dough developing time, stability time, noodle score of dry white Chinese noodle, and bread score were 777 g L-1, 12.3%, 25.1%, 32.9 mL, 326 s, 56.5%, 3.0 min, 4.5 min, 78.5, and 62.2, respectively. In a combined analysis of variance, the effects of genotype, environment, and their interaction were significant for almost all traits tested. For test weight, Zeleny sedimentation, falling number, flour water abstraction, and noodle score of dry white Chinese noodle, the influences between years were greater than differences between locations; whereas, for protein content, wet gluten content, dough developing time, stability time, and bread score, location had a greater influence than year. Increasing nitrogen rate significantly improved most quality traits and raised bread score, but had no influence on noodle score. Stability analysis revealed that Chuanmai 39 was stable for bread quality with the highest bread score value, while Chuanmai 37 was stable for most quality traits with the highest noodle score. This result indicates that Chuanmai 39 and Chuanmai 37 can be favorably used in wheat quality improvement in Sichuan Province. © 2010 Crop Science Society of China and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. Source

Lin L.,Sichuan Agricultural University | Shi J.,Mianyang Academy of Agricultural science | Liu Q.,Shandong Academy of Agricultural Sciences | Liao M.,Sichuan Agricultural University | Mei L.,Sichuan Agricultural University
Environmental Monitoring and Assessment | Year: 2014

In a preliminary study, we found that the cadmium (Cd) concentrations in shoots of the winter farmland weeds Cardamine hirsuta Linn. and Gnaphalium affine D. Don exceeded the critical value of a Cd-hyperaccumulator (100 mg kg-1), indicating that these two farmland weeds might be Cd-hyperaccumulators. In this study, we grew these species in soil containing various concentrations of Cd to further evaluate their Cd accumulation characteristics. The biomasses of C. hirsuta and G. affine decreased with increasing Cd concentrations in the soil, while the root/shoot ratio and the Cd concentrations in shoot tissues increased. The Cd concentrations in shoots of C. hirsuta and G. affine reached 121.96 and 143.91 mg kg-1, respectively, at the soil Cd concentration of 50 mg kg-1. Both of these concentrations exceeded the critical value of a Cd-hyperaccumulator (100 mg kg-1). The shoot bioconcentration factors of C. hirsuta and G. affine were greater than 1. The translocation factor of C. hirsuta was less than 1 and that of G. affine was greater than 1. These findings indicated that C. hirsuta is a Cd-accumulator and G. affine is Cd-hyperaccumulator. Both plants are distributed widely in the field, and they could be used to remediate Cd-contaminated farmland soil in winter. © 2014 Springer International Publishing. Source

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