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Hosūr, India

Nanda A.,Sathyabama University | Raghavan C.M.,Mgr College
International Journal of ChemTech Research | Year: 2014

Exopolysacharides (EPS) are polymeric substances of microorganisms of high molecular weight and long chain composed of sugar residues secreted by them into the surrounding environment. Bacterial EPS are also complex mixture of macro molecular poly electrolytes including polysaccharides, proteins and nucleic acids, each comprising of variable molecular mass and structural properties. Three bacteria viz., Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa were isolated from the Micro. Laboratory sinks of Pharmaceutical company. They were inoculated in EPS yeast mannitol glucose broth, as this medium promotes the production of exo-cellular polysaccharide. The pH of the medium was adjusted at 6.9+/- 0.2, later sterilized at 121degree for 15 min/15lbs. The dry weight of the fractionated products were found at 0.10g ± 0.02g/500ml, 0.41g ± 0.03g/500ml and 0.13g ± 0.05g/500ml of media produced from S. aureus, B. subtilis and P. aeruginosa in the YMGB broth respectively. During the study period, it was found that EPS extract was higher in Bacillus subtilis in comparison with other two. The physiochemical characterization of EPS was studied and the structure was confirmed by the FTIR and HPLC analysis for the B. subtilis only. From the Clinical Editor: In this study, bacteria isolated from adverse medical environment, particularly washing sink area, were subjected to EPS production and their (EPS) physiochemical characterization by FTIR and HPLC in order to get the structural confirmation. Source

Vimala S.,Mgr College
Rupkatha Journal on Interdisciplinary Studies in Humanities | Year: 2013

Examining select Anglo-Indian advice manuals written after the Indian Mutiny in 1857and during the 'high imperialism' period of the British Raj, the essay proposes that this cultural artefact served the purpose of constructing and naturalizing the English Memsahibs' gendered racial identity. By reiterating the performance of gender, class and race imperatives to construct a unique identity prerequisite for the Anglo-Indian community as well as the Indian colony, these texts aimed at the crystallization of this identity that will strengthen the idea of the British Raj. Such reiteration- apart from revealing the imperial anxiety of the subversion of the Memsahib identity- were useful to caution the English women new to the colonial environment. Reading these Anglo-Indian advice manuals produced for the consumption of the Anglo-Indian community, what the essay further proposes is that the performance of gendered-racial identity of the English women in India constituted not only the governance of their bodies and the Anglo-Indian spaces, but also their management of travel and material consumption including food. Judith Butler's Gender Trouble and Bodies That Matter provide useful insights to study the performance of the "politically correct Memsahib" identity and its attendant relation to the imagining of the homogenous British Raj. © Rupkatha Journal on Interdisciplinary Studies in Humanities. Source

Sathya V.,Mgr College | Abdul Samath J.,Coimbatore Institute of Technology
Asian Journal of Information Technology | Year: 2014

National Automatic Toll Collection System (NATCS) using Radio Frequency Identification (RFID) tag contains electronically stored information which can be read from up to several meters away. The Electronic Toll Collection System (ETC) executes the automatic payments of transportation fee without stopping a toll gate in express ways. The proposed toll-plaza will be act as a multi-plaza. Vehicles are classified with weight and speed. Multi-plaza takes the benefits of controlling the overloading, identifying theft. Operations Research Methods are used for the study of design and optimization of transportation systems. This study proposes an Optimal Queuing Model to minimize the queue and average waiting time of the vehicles in toll booths. The design can be further developed to aid the Satellite Surveillance Systems once all toll gates are networked. Experimental results signify that the proposed approach with ETC Smart Card Systems doubled the accuracy and produced adequate results. © Medwell Journals, 2014. Source

Nanda A.,Sathyabama University | Raghavan M.,Mgr College
Proceedings of the International Conference on Nanoscience, Engineering and Technology, ICONSET 2011 | Year: 2011

In order to find out the solution to prevent the growth of antibiotic resistant bacteria as well as the clinical isolates of enterobacteriaceae family pathogens, zinc sulphate bionanoparticles were synthesized from one of the airborne allergenic fungus viz., Aspergillus niger isolated from indoors of the MGR College, Hosur, TN, India during 2010. Antibacterial assay of such zinc nanoparticles were analyzed against enterobacteriaceae pathogens i.e., Escherichia coli, Klebsiella pneumonia, Shigella dysenteriae, Salmonella typhii and Proteus mirabilis through minimum inhibitory concentration and well diffusion methods. For the present study, clinical isolates of above said bacteria were obtained from Government General Hospital, Hosur, TN and later confirmed by various biochemical observations. Inhibition of pathogenic organisms was found in all the tubes from 1:2 to 1:512 dilutions. Zinc nanoparticles showed higher inhibition effect at different concentrations. Shigella dysenteriae was found as the susceptible clinical isolate to the zinc nanoparticles at all the concentrations 25 μl-100 μl. Likewise P. mirabilis (16mm) was more inhibited at 25μl concentration, S. typhii (21mm) at 50μl, E. coli (20mm) at 75 μl and P. mirabilis (25mm) as well as S. typhii (24mm) were found sensitive to the zinc nanoparticles at 100μl concentration. Other bacterial isolates were more or less sensitive to the inhibition effect. © 2011 IEEE. Source

Rajamurugan R.,Mgr College | Thirunavukkarasu C.,Pondicherry University | Sakthivel V.,Mgr College | Sivashanmugam M.,Mgr College | Raghavan C.M.,Mgr College
International Journal of Pharma and Bio Sciences | Year: 2013

The aim of the present study was to screen phytochemicals, antioxidant and antimicrobial activities of the extract from Tecoma stans flowers. The ethanolic extract of T. stans flowers demonstrated antioxidant activity in DPPH radical and β-carotenebleaching models. The extract exhibited a dose dependent reductive ability. The ethanolic extract of flowers of this species showed strong antimicrobial activity also and was effective against tested bacteria (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae) and fungi (Penicillium sp.). The extract was not effective against Aspergillus flavus, Cladosporium sp. and Botrytis sp. The phytochemical screening revealed the presence of tannins, flavonoids, phenols, alkaloids, steroids, cardiac glycosides and saponins in the flower extract. The present findings provide scientific evidence to support the traditional use of Tecoma stans and also indicate that the flowers of this species are a promising potential for the development of antioxidant and antimicrobial agents. Source

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