Silambarasu A.,Mgr College |
Manikandan A.,Bharath University |
Balakrishnan K.,P.A. College
Journal of Superconductivity and Novel Magnetism | Year: 2017
Spinel ZnFe2O4 nanoparticles (NPs) were successfully synthesized by a simple microwave irradiation method (MIM) using glycine as the fuel. For the comparative study purpose, it was also prepared by a conventional heating (CHM) method. Powder X-ray diffraction (XRD), Fourier transform infrared (FT-IR), high-resolution scanning electron microscope (HR-SEM), high-resolution transmission electron microscope (HR-TEM), energy-dispersive X-ray (EDX) spectra, and selected area electron diffraction (SAED) analysis showed that the samples were pure-phase spinel ZnFe2O4 nanoparticle-like morphology without any other secondary-phase impurity. UV-Visible diffuse reflectance spectra (DRS) and room temperature photoluminescence (PL) spectra confirmed the optical band gap (Eg) and defect state of the samples. The calculated Eg values of the samples are 1.98 and 2.11 eV for ZnFe2O4-CHM and ZnFe2O4-MIM, respectively. Vibrating sample magnetometer (VSM) analysis shows that the Ms value is 37.66 emu/g for ZnFe2O4-MIM, which is higher than the ZnFe2O4-CHM (24.23 emu/g) sample, which confirms that both the products showed a superparamagnetic behavior. ZnFe2O4-MIM was found to have a higher surface area than ZnFe2O4-CHM, which in turn leads to the improved performance towards the photocatalytic degradation (PCD) of methylene blue (MB), and it was found that the sample ZnFe2O4-MIM shows a higher PCD efficiency (91.43%) than ZnFe2O4-CHM (84.65%); also, the samples show high activity, good reusability, remarkable stability, and environmentally friendly materials for industrial and technological applications. © 2017 Springer Science+Business Media New York
Mrudula S.,Mgr College |
Reddy G.,Osmania University |
Seenayya G.,Issar Pharmaceuticals Pvt. Ltd
Malaysian Journal of Microbiology | Year: 2011
A highly thermostable amylopullulanase was purified to homogeneity from the culture filtrate of the Clostridium thermosulfurogenes SVM17. On SDS-PAGE, the purified fraction having both amylase and pullulanase activities were observed as a single band. The molecular weight of the purified amylopullulanase on SDS-PAGE was 97 kDa. The optimum temperature for both amylase and pullulanase was 70 °C. The enzyme was completely stable at 70 °C for 2 h. The presence of 5% starch increased the thermal stability of the enzyme at 100 °C up to 2 h. Both amylase and pullulanase activities were optimum at pH 5.5 to 6.0 and were stable over a pH range of 4.0 to 6.5. The TLC analysis of the reaction products on starch showed that maltose was the main product along with trace amounts of glucose. The analysis of hydrolysis product of pullulan showed that maltotriose was the main product. At 5 mM concentration, Mn2+ and Ag+ strongly stimulated both amylase and pullulanase activities, where as Mg2+, Ca2+, Cu2+, Fe3+, Zn2+, Hg2+, EDTA, Cd2+ and Li2+ inhibited both amylase and pullulanase activities. When the concentration of metal ions was increased from 5 to10 mM, a further increase in amylase activity was observed in the presence of Ni2+, Mn2+ and Co2+. Where as substantial decrease was observed at 10 mM concentration of Ag+, Pb2+ and Ca2+. © 2011. Malaysian Journal of Microbiology.
Nanda A.,Sathyabama University |
Raghavan C.M.,Mgr College
International Journal of ChemTech Research | Year: 2014
Exopolysacharides (EPS) are polymeric substances of microorganisms of high molecular weight and long chain composed of sugar residues secreted by them into the surrounding environment. Bacterial EPS are also complex mixture of macro molecular poly electrolytes including polysaccharides, proteins and nucleic acids, each comprising of variable molecular mass and structural properties. Three bacteria viz., Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa were isolated from the Micro. Laboratory sinks of Pharmaceutical company. They were inoculated in EPS yeast mannitol glucose broth, as this medium promotes the production of exo-cellular polysaccharide. The pH of the medium was adjusted at 6.9+/- 0.2, later sterilized at 121degree for 15 min/15lbs. The dry weight of the fractionated products were found at 0.10g ± 0.02g/500ml, 0.41g ± 0.03g/500ml and 0.13g ± 0.05g/500ml of media produced from S. aureus, B. subtilis and P. aeruginosa in the YMGB broth respectively. During the study period, it was found that EPS extract was higher in Bacillus subtilis in comparison with other two. The physiochemical characterization of EPS was studied and the structure was confirmed by the FTIR and HPLC analysis for the B. subtilis only. From the Clinical Editor: In this study, bacteria isolated from adverse medical environment, particularly washing sink area, were subjected to EPS production and their (EPS) physiochemical characterization by FTIR and HPLC in order to get the structural confirmation.
Sathya V.,MGR College |
Abdul Samath J.,Coimbatore Institute of Technology
Asian Journal of Information Technology | Year: 2014
National Automatic Toll Collection System (NATCS) using Radio Frequency Identification (RFID) tag contains electronically stored information which can be read from up to several meters away. The Electronic Toll Collection System (ETC) executes the automatic payments of transportation fee without stopping a toll gate in express ways. The proposed toll-plaza will be act as a multi-plaza. Vehicles are classified with weight and speed. Multi-plaza takes the benefits of controlling the overloading, identifying theft. Operations Research Methods are used for the study of design and optimization of transportation systems. This study proposes an Optimal Queuing Model to minimize the queue and average waiting time of the vehicles in toll booths. The design can be further developed to aid the Satellite Surveillance Systems once all toll gates are networked. Experimental results signify that the proposed approach with ETC Smart Card Systems doubled the accuracy and produced adequate results. © Medwell Journals, 2014.
Nanda A.,Sathyabama University |
Raghavan M.,MGR College
Proceedings of the International Conference on Nanoscience, Engineering and Technology, ICONSET 2011 | Year: 2011
In order to find out the solution to prevent the growth of antibiotic resistant bacteria as well as the clinical isolates of enterobacteriaceae family pathogens, zinc sulphate bionanoparticles were synthesized from one of the airborne allergenic fungus viz., Aspergillus niger isolated from indoors of the MGR College, Hosur, TN, India during 2010. Antibacterial assay of such zinc nanoparticles were analyzed against enterobacteriaceae pathogens i.e., Escherichia coli, Klebsiella pneumonia, Shigella dysenteriae, Salmonella typhii and Proteus mirabilis through minimum inhibitory concentration and well diffusion methods. For the present study, clinical isolates of above said bacteria were obtained from Government General Hospital, Hosur, TN and later confirmed by various biochemical observations. Inhibition of pathogenic organisms was found in all the tubes from 1:2 to 1:512 dilutions. Zinc nanoparticles showed higher inhibition effect at different concentrations. Shigella dysenteriae was found as the susceptible clinical isolate to the zinc nanoparticles at all the concentrations 25 μl-100 μl. Likewise P. mirabilis (16mm) was more inhibited at 25μl concentration, S. typhii (21mm) at 50μl, E. coli (20mm) at 75 μl and P. mirabilis (25mm) as well as S. typhii (24mm) were found sensitive to the zinc nanoparticles at 100μl concentration. Other bacterial isolates were more or less sensitive to the inhibition effect. © 2011 IEEE.
Rajamurugan R.,Mgr College |
Thirunavukkarasu C.,Pondicherry University |
Sakthivel V.,Mgr College |
Sivashanmugam M.,Mgr College |
Raghavan C.M.,Mgr College
International Journal of Pharma and Bio Sciences | Year: 2013
The aim of the present study was to screen phytochemicals, antioxidant and antimicrobial activities of the extract from Tecoma stans flowers. The ethanolic extract of T. stans flowers demonstrated antioxidant activity in DPPH radical and β-carotenebleaching models. The extract exhibited a dose dependent reductive ability. The ethanolic extract of flowers of this species showed strong antimicrobial activity also and was effective against tested bacteria (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae) and fungi (Penicillium sp.). The extract was not effective against Aspergillus flavus, Cladosporium sp. and Botrytis sp. The phytochemical screening revealed the presence of tannins, flavonoids, phenols, alkaloids, steroids, cardiac glycosides and saponins in the flower extract. The present findings provide scientific evidence to support the traditional use of Tecoma stans and also indicate that the flowers of this species are a promising potential for the development of antioxidant and antimicrobial agents.
Vimala S.,Mgr College
Rupkatha Journal on Interdisciplinary Studies in Humanities | Year: 2013
Examining select Anglo-Indian advice manuals written after the Indian Mutiny in 1857and during the 'high imperialism' period of the British Raj, the essay proposes that this cultural artefact served the purpose of constructing and naturalizing the English Memsahibs' gendered racial identity. By reiterating the performance of gender, class and race imperatives to construct a unique identity prerequisite for the Anglo-Indian community as well as the Indian colony, these texts aimed at the crystallization of this identity that will strengthen the idea of the British Raj. Such reiteration- apart from revealing the imperial anxiety of the subversion of the Memsahib identity- were useful to caution the English women new to the colonial environment. Reading these Anglo-Indian advice manuals produced for the consumption of the Anglo-Indian community, what the essay further proposes is that the performance of gendered-racial identity of the English women in India constituted not only the governance of their bodies and the Anglo-Indian spaces, but also their management of travel and material consumption including food. Judith Butler's Gender Trouble and Bodies That Matter provide useful insights to study the performance of the "politically correct Memsahib" identity and its attendant relation to the imagining of the homogenous British Raj. © Rupkatha Journal on Interdisciplinary Studies in Humanities.
Dheepa R.,Mgr College |
Paranjothi S.,Mgr College
Emirates Journal of Food and Agriculture | Year: 2010
Banana is the premier fruit of the Asia and pacific region. It is the fourth most important food crop after rice, wheat and maize. In Tamil Nadu, banana is grown in 92,200 hectares with an annual production of 48.56 million tones. Among all biotic constraints, viruses cause huge economic loss to the crop. Some of the major diseases are caused by viruses which include Banana Bunchy Top Virus (BBTV), Cucumber Mosaic Virus (CMV), Banana Streak Virus (BSV), Banana Bract Mosaic Virus (BBMV) have been reported in our country. CMV virus concentration in host (Musa) tissue is very less. So it is necessary to transfer this virus to good propagation host such as Nicotiana sp for purification. The purpose of the study was the transmission of CMV infecting banana into propagation host either by aphid or mechanically. The transmission was confirmed through Direct Antigen Coating Enzyme Linked Immuno Sorbent Assay (DAC-ELISA).
PubMed | Mgr College
Type: Journal Article | Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] | Year: 2013
Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase & FPase activities were found to be 3.29 and 2.3 U per ml culture broth, respectively. The productivity of extracellular cellulase in SSF was 14.6 fold higher than in SmF. The physical and nutritional parameters of fermentation like pH, temperature, substrate, carbon and nitrogen sources were optimized. The optimal conditions for maximum biosynthesis of cellulase by A. niger were shown to be at pH 6, temperature 30 C. The additives like lactose, peptone and coir waste as substrate increased the productivity both in SmF and SSF. The moisture ratio of 1:2 (w/v) was observed for optimum production of cellulase in SSF.