METs Institute of Pharmacy

Adgaon, India

METs Institute of Pharmacy

Adgaon, India
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Kasar N.S.,Mets Institute of Pharmacy
Indian Drugs | Year: 2016

The aim of the present research work was to investigatation and content estimation of quercetin in Psidium Guajava leaves extract. Psidium guajava Linn (Myrtaceae) has been used as traditional medicine.comparative study of hydroalcoholic and methanolic extract of Psidium guajava leaves was carried out. From IR charecterisation, TLC and UV determination of standard quercetin found that the quercetin is present in Psidium Guajava leaves extract and content of quercetin in hydroalcoholic extract was found to be 9.1 μg/mL and in methanolic extract found to be 7.55 μg/mL. Quercetin is detected in Psidium Guajava leaves extract by IR, UV and TLC analysis. © 2013 by Indian Drugs Online.


Dashputre N.L.,METs Institute of Pharmacy
International Journal of PharmTech Research | Year: 2010

OBJECTIVE: To study the immunomodulatory activity of aqueous and ethanolic extracts of leaves of Ocimum basilicum Linn. (Family: Lamiaceae) in mice. METHODS: The aqueous and ethanolic extract of leaves of Ocimum basilicum was administered orally at the dosage levels of 400 mg/kg/day body weight in mice. The assessment of immunomodulatory activity on specific and nonspecific immunity were studied by haemagglutination antibody (HA) titer, delayed type hypersensitivity (DTH), neutrophil adhesion test and carbon clearance test. In order to induced immunosuppresion in mice by using cyclophosphamide (100 mg/kg/day, p.o.) and levamisole (50 mg/kg/day, p.o.) used as immunostimulating agents. RESULTS: Oral administration of Ocimum basilicum (OB) showed a significant increase in the production of circulating antibody titer in response to sheep red blood cells (SRBCs). A significant (p<0.01) increase in both primary and secondary HA titer was observed while compared to control group, whereas in cyclophosphamide treated group OB showed significant (p<0.01) increase in HA titer. OB showed significantly (p<0.01) potentiated the DTH reaction by facilitating the footpad thickness response to SRBCs in sensitized mice. Also OB evoked a significant (p<0.01) increase in percentage neutrophil adhesion to nylon fibres and phagocytic activity. CONCLUSION: The study demonstrates that OB triggers both specific and non-specific responses to a greater extent. The study comprised the acute toxicity and preliminary phytochemical screening of OB. From the results obtained and phytochemical studies the immunostimulant effect of OB could be attributed to the flavonoid content.


Dharankar S.D.,Mets Institute Of Pharmacy | Parakh D.R.,Mets Institute Of Pharmacy | Patil M.P.,Mets Institute Of Pharmacy | Kshirsagar S.J.,Mets Institute Of Pharmacy
Drying Technology | Year: 2015

Ketoprofen is classified as BCS class II drug with low aqueous solubility, short half-life (2-2.5 h), and low oral bioavailability and also causes gastric irritation. The systems intended for oral administration were prepared by using soybean oil, Cremophor EL, and Maisine 35-1 and its adsorption on Aerosil 200. The spray-dried product demonstrated high process yield (52.2-68.3%) with good loading capacity (83.13-93.69%) and significant drug release compared to pure drug. The smooth spherical particles produced had no interaction with the excipients as demonstrated by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR). Differential scanning calorimetry (DSC) indicated the complete miscibility of the drug with excipients and X-ray diffraction (XRD) demonstrated the transformation from crystalline to amorphous form. © 2015 Taylor & Francis Group, LLC.


Patil S.D.,METs Institute of Pharmacy | Shelar K.E.,METs Institute of Pharmacy | Shakeel Z.H.,METs Institute of Pharmacy | Shaikh A.R.,METs Institute of Pharmacy
Der Pharma Chemica | Year: 2015

Two dimensional quantitative structure activity relationship (2D-QSAR) and three dimensional quantitative structure activity relationship (3D-QSAR) studies by means of Multiple Linear Regression (MLR), Partial Least Square (PLS) and Principal Component Regression (PCR) were performed on a series of 4-Alkoxycarbonyl-1, 5-diaryl-1, 2, 3-triazole analogues for the treatment of drug abuse disorder using software MDS 4.2 version (VLife Science). This study was performed with 13 compounds (Data set) using random as well as manual data selection methods for the division of the data into training and test set. MLR methodology with stepwise (SW) forwardbackward variable selection method was used for building the QSAR models. Statistically significant QSAR models were generated. Among them most significant model has squared correlation coefficient (r2) = 0.9895, cross validated correlation coefficient (q2) = 0.9747 and predictive correlation coefficient (pred_r2) = 0.9826 for the treatment of drug abuse disorders. The core idea of the present study is the search for novel 4-Alkoxycarbonyl-1, 5-diaryl-1, 2, 3-triazole analogues that would show promise to be useful in the treatment of drug abuse disorders.


Bedadurge A.B.,METs Institute of Pharmacy | Shaikh A.R.,METs Institute of Pharmacy
Journal of the Korean Chemical Society | Year: 2013

Quantitative structure-activity relationship (QSAR) analysis for recently synthesized imidazole-(benz)azole and imidazole - piperazine derivatives was studied for their anticancer activities against breast (MCF-7) cell lines. The statistically significant 2D-QSAR models (r2 = 0.8901; q2 = 0.8130; F test = 36.4635; r2 se = 0.1696; q2 se = 0.12212; pred-r2 = 0.4229; pred-r2 se = 0.4606 and r 2 = 0.8763; q2 = 0.7617; F test = 31.8737; r2 se = 0.1951; q2 se = 0.2708; pred-r2 = 0.4386; pred-r 2 se = 0.3950) were developed using molecular design suite (VLifeMDS 4.2). The study was performed with 18 compounds (data set) using random selection and manual selection methods used for the division of the data set into training and test set. Multiple linear regression (MLR) methodology with stepwise (SW) forward-backward variable selection method was used for building the QSAR models. The results of the 2D-QSAR models were further compared with 3D-QSAR models generated by kNN-MFA, (k-Nearest Neighbor Molecular Field Analysis) investigating the substitutional requirements for the favorable anticancer activity. The results derived may be useful in further designing novel imidazole-(benz)azole and imidazole-piperazine derivatives against breast (MCF-7) cell lines prior to synthesis.


Ahirrao S.,METs Institute of Pharmacy | Gide P.,METs Institute of Pharmacy | Shrivastav B.,Jaipur National University | Sharma P.,Jaipur National University
Particulate Science and Technology | Year: 2014

Theophylline is the most useful bronchodilators for the treatment of severe reversible bronchospasm. The fluctuations of serum theophylline level in clinical practice and associated central nervous system side effects necessitate the development of an extended release formulation. In the present study, theophylline-loaded beads were prepared by extruding the dispersion of theophylline, sodium alginate, and glycerol into the cationic crosslinking solution of calcium chloride. The effect of the addition of glycerol was determined by entrapment efficiency, drug release, and morphology of beads. Absence of chemical interaction between drug, polymer, and counterions after production of beads was confirmed by Fourier transform infrared spectroscopy. Theophylline entrapment of up to 72% was achieved in beads with almost spherical shape and size ranging from 0.67 to 1.12 mm. Percentage entrapment of theophylline found to be more and release was extended up to the eleventh hour from the glycerol containing sodium alginate beads. Hence, sodium alginate glycerol beads could represent a promising oral drug delivery system to extend the release of theophylline. Copyright © 2014 Taylor & Francis Group, LLC.


Kankate R.S.,METs Institute of Pharmacy | Vanjari S.S.,Institute of Chemical Technology
International Journal of ChemTech Research | Year: 2010

A series of 2'-hydroxychalcones and 3-hydroxyflavones were evaluated for in vitro antioxidant activity on four different models namely Diphenyl Picryl Hydrazine, Nitric Oxide, Hydrogen peroxide, and Phenyl hydrazine induced hemolysis of erythrocytes. The studies indicate that amongst evaluated 2'-hydroxychalcones, 2-Bromochalcone and 2-Chlorochalcones showed significant activity and amongst different 3-hydroxyflavone evaluated 3'-hydroxyflavones, 4'-methoxy and 4'-Chloroflavone showed significant activity.


Parakh D.R.,Mets Institute Of Pharmacy | Patil M.P.,Mets Institute Of Pharmacy | Dashputre N.L.,Mets Institute Of Pharmacy | Kshirsagar S.J.,Mets Institute Of Pharmacy
Drying Technology | Year: 2016

In this study, a novel liquid self-microemulsifying drug delivery system (SMEDDS) containing mebendazole was formulated and further developed into a solid form by a spray drying method using Aerosil 200 as the solid carrier. The optimum liquid SMEDDS consisted of Labrafil 2125 CS, Tween 20, and Maisine 35-1 as the oil phase, the surfactant, and the cosurfactant, respectively. The formulated SMEDDS was completely emulsified or dispersed within a minute. All formulations were dissolved within 1 h using 0.1 N HCl as dissolution medium, whereas pure drug was less significantly dissolved in this time period. The droplet size was found to be within 250 nm for solid forms of SMEDDS. Solid state characterization was performed by scanning electron micrograph, differential scanning calorimetry, and X-ray powder diffraction. After oral administration to Wistar rats, mebendazole in the solid SMEDDS resulted in the significant improvement in bioavailability compared with that of pure drug analyzed by RP-HPLC. The optimized formulation showed 24.87 folds increase in bioavailability as compared to pure drug and 8.39 folds increase in bioavailability in comparison to marketed tablet of mebendazole. The optimized batch has found to be 3.1726 years of shelf life. In conclusion, the solid SMEDDS is a promising solid dosage form for poorly water-soluble and low bioavailability drugs. © 2016, Copyright © Taylor & Francis Group, LLC.


Nikumbh K.V.,METs Institute of Pharmacy | Sevankar S.G.,METs Institute of Pharmacy | Patil M.P.,METs Institute of Pharmacy
Drug Delivery | Year: 2015

Background: Anti-inflammatory agents are widely used to relieve inflammation caused by various factors. Aim: This study was initiated with the intention to deliver low aqueous soluble ketoprofen to enhance its solubility by developing microemulsion system as a template and then incorporating it into gel phase. Materials and methods: Initially ketoprofen was solubilized into microemulsion preparation made up of clove oil, Tween 20 and propylene glycol as oil phase, surfactant and co-surfactant respectively, then it was incorporated into different concentration of gelling phase using gelling agents namely Carbopol 940, Carbopol 934 and hydroxypropyl methyl cellulose K4M (HPMC K4M). Formulated emulgels were evaluated for their physical appearance, pH, rheological properties, globule size, extrudability, drug content, spreadability, bioadhesion strength, in vitro and ex vivo drug release, skin irritation test and anti-inflammatory activity. Results: Microemulsion had shown globule size 396 nm, pH 6-6.7, viscosity 29.4 cps and zeta potential -12 mV indicating good stability. Formulated emulgels showed good physical appearance, skin acceptable pH 6-6.9, non-Newtonian shear thinning system, drug content 99.28 ± 0.16%, bioadhesion strength 48.4 gram force, globule size 473 nm, spreadability 22.96 gm.cm/s, good extrudability, in vitro release, ex vivo release did not showed any irritation reaction and possess a good anti-inflammatory activity. Conclusions: Selected batch showed enhanced drug release (92.42 ± 4.66%) as compared to marketed gel (65.94 ± 3.30). Similarly ex vivo release of formulation showed 72.22% release through mice skin compared with marketed gel. Formulations followed Korsmeyer-Peppas diffusion kinetic model. It was observed from the results that the formulated emulgel can provide promising delivery of ketoprofen. © 2013 Informa Healthcare USA, Inc. All rights reserved.


PubMed | METs Institute of Pharmacy
Type: Comparative Study | Journal: Drug delivery | Year: 2015

Anti-inflammatory agents are widely used to relieve inflammation caused by various factors.This study was initiated with the intention to deliver low aqueous soluble ketoprofen to enhance its solubility by developing microemulsion system as a template and then incorporating it into gel phase.Initially ketoprofen was solubilized into microemulsion preparation made up of clove oil, Tween 20 and propylene glycol as oil phase, surfactant and co-surfactant respectively, then it was incorporated into different concentration of gelling phase using gelling agents namely Carbopol 940, Carbopol 934 and hydroxypropyl methyl cellulose K(4)M (HPMC K(4)M). Formulated emulgels were evaluated for their physical appearance, pH, rheological properties, globule size, extrudability, drug content, spreadability, bioadhesion strength, in vitro and ex vivo drug release, skin irritation test and anti-inflammatory activity.Microemulsion had shown globule size 396nm, pH 6-6.7, viscosity 29.4cps and zeta potential -12mV indicating good stability. Formulated emulgels showed good physical appearance, skin acceptable pH 6-6.9, non-Newtonian shear thinning system, drug content 99.280.16%, bioadhesion strength 48.4 gram force, globule size 473nm, spreadability 22.96gm.cm/s, good extrudability, in vitro release, ex vivo release did not showed any irritation reaction and possess a good anti-inflammatory activity.Selected batch showed enhanced drug release (92.424.66%) as compared to marketed gel (65.943.30). Similarly ex vivo release of formulation showed 72.22% release through mice skin compared with marketed gel. Formulations followed Korsmeyer-Peppas diffusion kinetic model. It was observed from the results that the formulated emulgel can provide promising delivery of ketoprofen.

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