Da Luz M.H.M.,University of Sao Paulo |
Da Luz M.H.M.,Methodist University of Sao Paulo |
Peres I.T.,University of Sao Paulo |
Santos T.G.,International Research Center |
And 3 more authors.
Frontiers in Cellular Neuroscience | Year: 2015
Accumulation of protein aggregates is a histopathological hallmark of several neurodegenerative diseases, but in most cases the aggregation occurs without defined mutations or clinical histories, suggesting that certain endogenous metabolites can promote aggregation of specific proteins. One example that supports this hypothesis is dopamine and its metabolites. Dopamine metabolism generates several oxidative metabolites that induce aggregation of a-synuclein, and represents the main etiology of Parkinson's diseases. Because dopamine and its metabolites are unstable and can be highly reactive, we investigated whether these molecules can also affect other proteins that are prone to aggregate, such as cellular prion protein (PrPC). In this study, we showed that dopamine treatment of neuronal cells reduced the number of viable cells and increased the production of reactive oxygen species (ROS) as demonstrated in previous studies. Overall PrPC expression level was not altered by dopamine treatment, but its unglycosylated form was consistently reduced at 100 μiM of dopamine. At the same concentration, the level of phosphorylated mTOR and 4EBP1 was also reduced. Moreover, dopamine treatment decreased the solubility of PrPC, and increased its accumulation in autophagosomal compartments with concomitant induction of LC3-II and p62/SQSTM1 levels. In vitro oxidation of dopamine promoted formation of high-order oligomers of recombinant prion protein. These results suggest that dopamine metabolites alter the conformation of PrPC, which in turn is sorted to degradation pathway, causing autophagosome overload and attenuation of protein synthesis. Accumulation of PrPC aggregates is an important feature of prion diseases. Thus, this study brings new insight into the dopamine metabolism as a source of endogenous metabolites capable of altering PrPC solubility and its subcellular localization. © 2015 da Luz, Peres, Santos, Martins, Icimoto and Lee.
Ramos R.T.,Methodist University of Sao Paulo
AJOB Neuroscience | Year: 2011
This article proposes a recontextualization of the conception of free will in terms of social events rather than in mental experiences. My objective is to demonstrate that the behavior of human social groups composed of individuals freely interacting among each other exhibits well-defined patterns that can precisely be described by a physics formalism. Although not directly approaching the nature of the mental states involved in each individual behavior, I argue that the emergence of organized social behavior occurs under the influence of free will. First, I briefly introduce the concept of self-organized criticality (SOC) that is central to the understanding of my argument. Second, I review experimental data showing that normal individuals and patients with differentmental disorders exhibit a social behavior pattern compatible with the presence of SOC. Third, a brief discussion about the functioning of free will in psychiatric disorders is presented. Finally, the implications for psychological and social theories of including free will in this kind of physical model are discussed. © Taylor & Francis Group, LLC.
Pinto A.C.,Methodist University of Sao Paulo |
Mattioli G.V.,Methodist University of Sao Paulo
SAE Technical Papers | Year: 2014
This paper describes the development of an Intelligent Traffic Lights Control System using Fuzzy Logic concepts. Fuzzy Logic offers the possibility to 'compute with words', by using a mechanism for representing linguistic constructs common on real world problems. This is very important when the complexity of a task (problem) exceeds a certain threshold. Real world complex problems such human controlled systems involve a certain degree of uncertainty, which cannot be handled by traditional binary set theory. The algorithm implementation will be done inside Mathworks MATLAB software, and the results will be measured on Simulink Tool to create traffic scenarios and comparisons between simple time-based algorithms and the proposed system. During Project phase, a Robustness Parameter-Diagram will be used to design the system and cover its variables, error states, possible noises and control factors. The Project also comprehends some research regarding the currently used algorithms (including Artificial Intelligence) and technologies for Intelligent Traffic Light Control Systems. The benefits of using intelligent traffic lights are well known on society, and this paper can inspire academics and professionals to use different approaches to develop such systems. Copyright © 2014 SAE International.
Martinez S.,Methodist University of Sao Paulo
Mundo da Saude | Year: 2013
According to the Food Guide for the Brazilian Population, from the Ministry of Health, adequate food depends on the consumption of food, not nutrients, and should be based on food practices that have social and cultural significance. For WHO/UN, despite many advances, there are nutritional deficiencies affecting large numbers of people around the world. The world is plagued by an epidemic of obesity and chronic non-communicable diseases; among silent diseases there are also eating disorders. Many authors have said that food consumption and the way in which this happens has varied greatly in recent decades in the light of social changes that have taken place, mainly the changing role of women in the formation and maintenance of a family structure. A good program to favour quality of life should include a rigorous planning of actions with a good diagnostic of current social, physical and health condition of workers before any effective action. Food can and should help to improve the quality of life both physically and mentally and socially. Food is factor that greatly contributes for a happy and dynamic society that constantly progresses.
Basile L.F.,Methodist University of Sao Paulo
PloS one | Year: 2010
In this study we analyzed the topography of induced cortical oscillations in 20 healthy individuals performing simple attention tasks. We were interested in qualitatively replicating our recent findings on the localization of attention-induced beta bands during a visual task , and verifying whether significant topographic changes would follow the change of attention to the auditory modality. We computed corrected latency averaging of each induced frequency bands, and modeled their generators by current density reconstruction with Lp-norm minimization. We quantified topographic similarity between conditions by an analysis of correlations, whereas the inter-modality significant differences in attention correlates were illustrated in each individual case. We replicated the qualitative result of highly idiosyncratic topography of attention-related activity to individuals, manifested both in the beta bands, and previously studied slow potential distributions . Visual inspection of both scalp potentials and distribution of cortical currents showed minor changes in attention-related bands with respect to modality, as compared to the theta and delta bands, known to be major contributors to the sensory-related potentials. Quantitative results agreed with visual inspection, supporting to the conclusion that attention-related activity does not change much between modalities, and whatever individual changes do occur, they are not systematic in cortical localization across subjects. We discuss our results, combined with results from other studies that present individual data, with respect to the function of cortical association areas.
Da Silva Sousa M.V.,Methodist University of Sao Paulo |
Scanavini M.A.,Methodist University of Sao Paulo |
Sannomiya E.K.,Methodist University of Sao Paulo |
Velasco L.G.,Methodist University of Sao Paulo |
Angelieri F.,Methodist University of Sao Paulo
Photomedicine and Laser Surgery | Year: 2011
Introduction: This study evaluated the effect of low-level laser irradiation on the speed of orthodontic tooth movement of canines submitted to initial retraction. Methods: Twenty-six canines were retracted by using NiTi spring (force of 150 g/side). Thirteen of those were irradiated with diode laser (780 nm, 20 mW, 10 sec, 5 J/cm2) for 3 days, and the other 13 were not irradiated and thus were considered the control group. Patients were followed up for 4 months, and nine laser applications were performed (three each month). The movement of the canines was evaluated through 3D casts, and the statistical analysis was performed with ANOVA and Tukey tests (p < 0.05). Periapical radiographs of the studied teeth were submitted to Levander, Malmgreen, and alveolar bone ridge analyses to evaluate tissue integrity and were compared with the Wilcoxon test (p < 0.05). Results: A statistically significant increase in the movement speed of irradiated canines was observed in comparison with nonirradiated canines in all evaluation periods. No statistically significant difference was observed in bone and root resorption of canines, whether irradiated or not. Conclusion: The diode laser used within the protocol guidelines increased the speed of tooth movement. This might reduce orthodontic treatment time. Copyright © 2011, Mary Ann Liebert, Inc.
Barbosa J.L.R.,Methodist University of Sao Paulo |
Junior D.B.,Methodist University of Sao Paulo
Revista Neurociencias | Year: 2011
Introduction. Spinal cord injury is considered serious disabling neurological syndrome, occurs mainly in young adults. The heart rate variability has been used as a noninvasive means to assess the neural control of heart analyzing fluctuations. Objective. To assess heart rate variability in patients with spinal cord injury underwent an incremental test of upper limbs. Method. Four male subjects with a diagnosis of spinal cord injury performed an incremental test to maximal effort on a cycle ergometer of upper extremities. All records of heartbeats were made with the use of a heart rate monitors (Polar® S810 Heart Rate Monitor). These recorded beats were directed to a computer through an interface IR emission of infrared signals, so that they could carry out analysis of heart rate variability by using the Polar Precision Performance software. Results. The values of heart rate variability are greater in individuals with spinal cord injury highest (T1) compared with individuals with spinal cord injury lowest (T3, T6, T10). Conclusion. The variability of heart rate was higher in individuals with SCI at T1 when compared with individuals with lower TRM (T3, T6, T10).
Nakaya L.,Methodist University of Sao Paulo |
Sa C.S.C.,Methodist University of Sao Paulo
Revista Neurociencias | Year: 2013
Cerebral palsy (CP) refers to neuromuscular deficit caused by a non-progressive lesion of the immature brain resulting in a clinical variety, but presenting a common motor and sensory integration deficit. Objective. The aim of this descriptive study was to compare the performance of balance between hemiparetic spastic children with CP classified at Gross Motor Function Classification System (GMFCS) level I and normal development children in the same age by dynamic posturography. Results. As a result, we find similarity between both groups for the variables of postural stability and adjustment, and we could see greater difficulty of children with CP to maintain and restores balance in the anteroposterior axis, although the CP group under some conditions, performed better when compared to children in normal development. Conclusion. We conclude that the similarities found are due to the fact that both groups were still in neuronal maturation of postural control, and that children with CP had greater sway and displacement of the center of pressure in the anteroposterior direction.
Joias R.P.,Methodist University of Sao Paulo
World journal of orthodontics | Year: 2010
To evaluate the Bolton ratios in Brazilians with natural, normal occlusions. Thirty-five dental casts of Brazilian Caucasians with a natural, normal occlusion from the files of the Postgraduate Program in Orthodontics of the Methodist University of São Paulo were scanned in three dimensions. On the scanned images, the greatest mesiodistal distance of each tooth from right first molar to left first molar was measured with Geomagic Studio 5 software. The Bolton ratios were then calculated. The anterior ratio amounted to 78.66% (SD ± 2.72) and the total ratio to 91.58% (SD ± 2.20). The anterior, but not the overall, ratio was significantly different from the ratio suggested by Bolton. COPYRIGHT © 2009 BY QUINTESSENCE PUBLISHING CO, INC.
Renders H.,Methodist University of Sao Paulo
Historia, Ciencias, Saude - Manguinhos | Year: 2012
The article compares William Hogarth's Gin Lane and Beer Street with texts by his contemporary John Wesley, spiritus rector of the Methodist movement. Although Hogarth had portrayed the Methodist religious movement in some of his prints as dangerously enthusiastic, both men defended a similar agenda concerning alcohol consumption. The similarities in their two ways of understanding the issue can be explained by culturally established customs, while distinct social outlooks can account for their differences.