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PubMed | Ajou University, Surgery., Medical Genetics and. and Endocrinology and Metabolism and.
Type: Evaluation Studies | Journal: American journal of clinical pathology | Year: 2015

We evaluated the utility of the VE1 antibody that can detect a mutant protein resulting from the BRAF V600E mutation as a diagnostic tool for thyroid fine-needle aspiration cytology (FNAC).We performed VE1 immunocytochemistry on 202 FNAC specimens from surgically confirmed thyroid nodules. The results were compared with the molecular analyses of the BRAF mutation in these specimens matched with their corresponding histology.Diagnoses of FNAC specimens included benign (9.4%), atypia of undetermined significance/follicular lesion of undetermined significance (11.4%), follicular neoplasm/suspicious for follicular neoplasm (2.0%), suspicious for malignancy (9.4%), and malignancy (65.8%). VE1 immunostaining was positive in 71.3% of FNAC specimens. The overall sensitivity of the VE1 antibody was 88.8%, specificity was 71.2%, positive predictive value was 88.2%, negative predictive value was 72.4%, and diagnostic accuracy was 83.7%.VE1 immunocytochemistry in thyroid FNAC as a screening test for BRAF mutations is highly specific for malignant category cases but can be suboptimal due to its high false-positive rate for the nonmalignant cases.

PubMed | Hyogo College of Medicine and Endocrinology and Metabolism and.
Type: Journal Article | Journal: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology | Year: 2015

The aim of this study in patients with gestational diabetes mellitus (GDM) was to evaluate the relationship of insulin resistance and secretion to area-under-the-sensor glucose concentration-time curve from before to 120min postmeal (CGM-AUC(0-120min)) as determined with continuous glucose monitoring (CGM).Immunoreactive insulin and HbA1c were determined in 22 Japanese patients with GDM undergoing a 75g oral glucose tolerance test. Patients underwent CGM within 3 weeks of receiving a diagnosis of GDM.HbA1c (NGSP) was 5.50.4%, BMI was 24.85.3kg/m(2), mean sensor glucose by CGM was 94.210.3mg/dL, standard deviation was 17.54.4mg/dL, and CGM-AUC(0-120min) was 204.223.8hmg/dL. The insulin resistance indices the homeostasis model assessment ratio (HOMA-R), quantitative insulin sensitivity check index (QUICKI), and the Matsuda Index were correlated with CGM-AUC(0-120min). The disposition index (DI), which was used to evaluate insulin secretion, was negatively correlated with CGM-AUC(0-120min).Not only insulin resistance but also beta cell dysfunction contributes to postprandial hyperglycemia in Japanese patients with GDM.

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