Yang Y.-L.,Chang Gung Memorial Hospital |
Chang C.-H.,En Chu Kong Hospital |
Huang C.-C.,Ming Chuan University |
Huang C.-C.,Regional Development Centre |
And 4 more authors.
Bio-Medical Materials and Engineering | Year: 2014
Numerous materials have been proposed for bone tissue engineering. In this study, a newly designed hybrid composite scaffold composed of poly (D,L-lactide-co-glycolide) and a naturally bioceramic hybrid material, nanonized pearl powder, were prepared and the biological activities and physical properties of the scaffold for bone tissue engineering were evaluated. It is a composite consisting calcium carbonate crystal in an aragonite structure, embedded in an organic matrix. Peral contains one or more signal molecules capable of stimulating bone formation. The nanonized pearl powder is considered as a promising osteoinductive biomaterial. This biomaterial is biocompatible and shows osteogenic activity. In this study, the designed biohybrid of nanonized pearl powder/poly (lactide-co-glycolide) (NPP/PLGA) biocomposite scaffolds would employ biodegradable material as MC3T3-E1 cells seeded scaffolds. Therefore, the biocomposite scaffolds would be used to culture with MC3T3-E1 cells under spinner bioreactor in vitro. Furthermore, it also detailed how these tissues were characterized, qualitatively and quantitatively, with scanning electron microscopy and biochemical testing. The identity and the mode of action of these molecules on the osteoblast differentiation were analyzed. This study indicates that the efficiency of nanonized pearl powders in bone cell differentiation are certainly different from that of proteins. Further sudy will look forward to manufacturing the promising new generation bone substitute, three dimensional biocomposite scaffolds to replace the implant and autogeneous bone graft, which combines basic research and clinical application. © 2014 - IOS Press and the authors. All rights reserved.
Yang S.-C.,National Chung Hsing University |
Kao W.M.,MesoPhase Technologies Inc |
Kao W.M.,National Taiwan University |
Wu C.-J.,MesoPhase Technologies Inc |
And 8 more authors.
African Journal of Biotechnology | Year: 2011
Pearl and nacre (mother of pearl) have similar chemical compositions. However, more than 20 proteins have been identified in nacre, yet none have been detected in pearl thus far. This study aimed to detect and identify protein in pearl. Two batches of pearls formed in Hyriopsis cumingii (Lea) were purchased from two pearl farms. They were ground into a powder of >10,000 mesh followed by ultra-sonication and extraction in water for 4 h at room temperature. The solution was centrifuged and the supernatant was saved as pearl powder water extract. A portion of the extract was heated at 121°C for 20 min. TCA precipitation and tricine-SDS-PAGE were conducted on both the heated and non-heated extracts. After silver nitrate staining, the heated extract demonstrated a distinct protein signal, but the non-heated extract did not. The protein band from each of the two heated extracts was excised from the gel and subjected to tryptic digestion and RP-nano-HPLC-ESI-MS/MS analysis. A MASCOT search of the results indicated that one protein had significant sequence homology to a putative vitelline envelop receptor for lysine in the common marine mussel Mytilus edulis, and the other to the putative imaginal disc growth factor (IDGF) of Diaprepes abbreviatus. © 2011 Academic Journals.
Yin S.-H.,National Cheng Kung University |
Wang C.-C.,National Cheng Kung University |
Cheng T.-J.,Chi Mei Medical Center |
Cheng T.-J.,Chia Nan University of Pharmacy and Science |
And 17 more authors.
Chinese Medicine (United Kingdom) | Year: 2012
Background: Xiao-Yao-San (XYS) is a Chinese medicinal formula for treating anxiety and depression. This study aims to evaluate the use of a room-temperature super-extraction system (RTSES) to extract the major active components of XYS and enhance their psycho-pharmacological effects.Methods: The neuroprotective roles of XYS/RTSES against reserpine-derived neurotoxicity were evaluated using a glial cell injury system (in vitro) and a depression-like C57BL/6 J mouse model (in vivo). The anxiolytic-behavioural effects were measured by the elevated plus-maze (EPM) test and the antidepressant effects were evaluated by the forced swimming test (FST) and tail suspension test (TST). Glucose tolerance and insulin resistance were assayed by ELISA. The expression of 5-HT1A receptors in the prefrontal cortex was examined by western blotting.Results: XYS/RTSES (300 μg/mL) diminished reserpine-induced glial cell death more effectively than either XYS (300 μg/mL) or fluoxetine (30 μM) at 24 h (P = 0.0481 and P = 0.054, respectively). Oral administration of XYS/RTSES (500 mg/kg/day) for 4 consecutive weeks significantly elevated the ratios of entries (open arms/closed arms; P = 0.0177) and shuttle activity (P = 0.00149) on the EPM test, and reduced the immobility time by 90% on the TST (P = 0.00000538) and FST (P = 0.0000053839). XYS/RTSES also improved the regulation of blood glucose (P = 0.0305) and increased the insulin sensitivity (P = 0.0093). The Western blot results indicated that the activation of cerebral 5-HT1A receptors may be involved in the mechanisms of XYS/RTSES actions.Conclusion: The RTSES could provide a novel method for extracting effective anxiolytic- and antidepressant-like substances. XYS/RTSES improved the regulation of blood glucose and increased the insulin sensitivity in reserpine-induced anxiety and depression. Neuroprotection of glial cells and activation of cerebral 5-HT1A receptors were also involved. © 2012 Yin et al.; licensee BioMed Central Ltd.
Mesophase Technologies Inc | Date: 2012-12-26
An extraction technology of a Xiao-yao-drink includes a medicine preparing step of preparing herbal medicines, a pulverizing step of pulverizing the herbal medicines to produce medicinal solid particles, a liquid extracting step of adding the medicinal solid particles into an extraction liquid to dissolve the medicinal solid particles in the extraction liquid, an ultrasonic extraction step of impacting the medicinal solid particles in the extraction liquid by concentrated ultrasonic waves to crush the medicinal solid particles into medicinal molecules, and a centrifugal filtering step of filtering the medicinal molecules from the extraction liquid by a strainer cloth to produce a medicinal extract which constructs a Xiao-yao-drink product. Thus, the extraction technology uses an ultrasonic extraction procedure to crush the medicinal solid particles into medicinal molecules by concentrated ultrasonic waves to efficiently increase the active component of the herbal medicine.
Mesophase Technologies Inc. | Date: 2010-04-13
essential oils, astringents for cosmetic purposes, beauty masks, cosmetics, cosmetic creams, perfumes, greases for cosmetic purposes, hair lotions, lotions for cosmetic purposes, medicated soap, nail polish, oils for perfumes and scents, shampoos, cosmetic preparations for skin care, skin whitening creams, cosmetic preparations for slimming purposes, namely, creams and lotions for cellulite reduction, cleansing milk for toilet purposes, make-up preparations, sunscreen preparations. dietetic beverages adapted for medical purposes; dietetic substances adapted for medical use, namely, nutritional supplements and food for babies; dietetic foods adapted for medical purposes; mineral food-supplements; nutritional additives for medical purposes for use in food and dietary supplements for human consumption; medical preparations for slimming purposes, namely, appetite suppressants, diet capsules and pills; vitamin preparations; pharmaceutical preparations for skin care; ointments for pharmaceutical purposes for the treatment of cancer; dried medicinal herbs; non-nutritive edible plant fibers for the treatment of constipation; lecithin for medical purposes; herb teas for medicinal purposes. machines and machine tools, namely, for the chemical, pharmaceutical, biochemical, cosmetic and biotechnological industries and the food industry, namely, machines and machine tools used for growing, processing, extracting, and storing bacteria, animal cells and plant cells for the production of biological products in the nature of vaccines, therapeutic and diagnostic preparations and proteins and food and cosmetic and component parts thereof, electromechanical machines for food preparation and cosmetic, centrifugal machines, flour mill machines, mixing machines, biodiesel machines, namely, mixing equipment, pumps and heaters.