PubMed | d Wildlife Information Liaison Development WILD Society, Western Regional Center, Mes Abasaheb Garware College, Indian Institute of Science and Government Science College
Type: | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016
Scorpions of the family Buthidae are widespread species in India. While studies are available on diversity and distribution of Indian buthid scorpions, no information is available on the phylogenetic relationships among the members of the family, within India and Asia in general. In the current study, we provide the first molecular phylogeny of buthid scorpions from central western India based on the mitochondrial cytochrome oxidase subunit I (COI) gene. Our analysis confirms the current placement of the species, previously assigned to Mesobuthus in the genus Hottentotta. However, the analysis also suggests that the member of this genus from India form a monophyletic group distinct from the members of Hottentotta from Africa. Species of Lychas formed a monophyletic group. Although Orthochirus was nested within the larger clade of buthidae comprising genera such as Androctonus, Buthacus, Buthus and Odontobuthus, the exact phylogenetic placement will require more taxonomic sampling of the known genera of Buthidae. We also show that there is a substantial genetic variation among the populations of medically important scorpion species Hottentotta tamulus, and the genetic distance is linearly correlated with the geographical distance between the populations.
Gauri B.,MES Abasaheb Garware College |
Vidya K.,MES Abasaheb Garware College |
Sharada D.,YM College |
Shobha W.,MES Abasaheb Garware College
Research Journal of Chemistry and Environment | Year: 2016
t A flexible room temperature ethanol gas sensor was fabricated using Ag/Ago nanoparticles as a sensing material. The synthesis of Ag/AgO nanoparticles was carried out by using sonochemical method with PAP apoenzyme as a stabilizing and capping agent. The Ag/AgO sensor has a detection limit of 10 ppm at room temperature and shows good sensitivity to ethanol. An extraction and isolation of PAP was carried from novel source Colocasia esculenta(arvi). The synthesized nano particles were analyzed for structural study using XRD, FESEM and UV-VIS spectroscopy. The XRD pattern shows the formation of Ag/ AgO nanoparticles. The FESEM images show spherical and plate like morphology with the average particle size in the range of 40-50 nm. The UV-VIS spectra show the broad peak at 428 nm, corresponding to Ag nanoparticles.
Dahanukar N.,Indian Institute of Science |
Dahanukar N.,Outreach |
Krutha K.,Wildlife Information Liaison Development WILD Society |
Paingankar M.S.,Outreach |
And 4 more authors.
PLoS ONE | Year: 2013
The Western Ghats of India harbors a rich diversity of amphibians with more than 77% species endemic to this region. At least 42% of the endemic species are threatened due to several anthropogenic stressors. However, information on amphibian diseases and their impacts on amphibian populations in this region are scarce. We report the occurrence of Batrachochytridium dendrobatidis (Bd), an epidermal aquatic fungal pathogen that causes chytridiomycosis in amphibians, from the Western Ghats. In the current study we detected the occurrence of a native Asian Bd strain from three endemic and threatened species of anurans, Bombay Night Frog Nyctibatrachus humayuni, Leith's Leaping Frog Indirana leithii and Bombay Bubble Nest Frog Raorchestes bombayensis, for the first time from the northern Western Ghats of India based on diagnostic nested PCR, quantitative PCR, DNA sequencing and histopathology. While, the Bd infected I. leithii and R. bombayensis did not show any external symptoms, N. humayuni showed lesions on the skin, browning of skin and sloughing. Sequencing of Bd 5.8S ribosomal RNA gene, and the ITS1 and ITS2 regions, revealed that the current Bd strain is related to a haplotype endemic to Asia. Our findings confirm the presence of Bd in northern Western Ghats and the affected amphibians may or may not show detectable clinical symptoms. We suggest that the significance of diseases as potential threat to amphibian populations of the Western Ghats needs to be highlighted from the conservation point of view. © 2013 Dahanukar et al.
PubMed | University of Pune, Bharati Vidyapeeths Medical College and Mes Abasaheb Garware College
Type: Journal Article | Journal: Journal de mycologie medicale | Year: 2016
The occurrence of yeast infections in humans has increased, with the species belonging to genus Candida still being the most common cause of infection. Nevertheless, infections caused by less common yeasts have been widely reported in recent years. The main objective of this study was to assess the potential of these less common saprophytic yeasts to invade the host cell, which is essential for causing systemic infections.Various yeast isolates were identified by DNA sequence information of PCR amplified ITS region. The purported saprophytic yeasts were characterized for internalization by mammalian cells in vitro, by staining the F-actin.The identification of different yeast isolates from various patients revealed that 70% of the isolates belonged to the genus Candida, while remaining 30% of the isolates were yeasts not belonging to genus Candida. These non-Candida clinical isolates, either in yeast or hyphal forms, were efficiently internalized by human epithelial cells. The internalization was marked by a process of actin polymerization surrounding the invading yeast. Such uptake by epithelial cells signifies traversal of cell barrier by yeast cells during infection in vivo.
Shintre S.,University of Pune |
Shintre S.,Mes Abasaheb Garware College |
Thakur P.,University of Pune
Indian Journal of Chemical Technology | Year: 2016
Study Ag-TiO2 composite has been synthesized by photodeposition method. The prepared composite has been characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and diffuse reflectance spectroscopy (DRS). The effect of Ag deposition on TiO2 has been tested for photocatalytic degradation of azo dye, Evans Blue (EB). The comparison of different advanced oxidation processes (AOP) such as (i) Only Ag-TiO2(ii) only UV (iii) UV + TiO2 (iv) UV + Ag-TiO2 (v) UV + Ag-TiO2 +H2O2 have been carried out for the photocatalytic degradation of EB. In addition, different operational parameters like initial concentration of EB and composite dose have also been studied on photocatalytic degradation of EB. The degradation is found to increase in the order only Ag-TiO2 < Only UV < UV + TiO2 < UV + Ag-TiO2 < UV + Ag-TiO2 + H2O2 for EB. © 2016, National Institute of Science Communication and Information Resources (NISCAIR). All Rights Reserved.
Badave K.D.,University of Pune |
Badave K.D.,MES Abasaheb Garware College |
Khan A.A.,University of Pune |
Rane S.Y.,University of Pune
Anti-Cancer Agents in Medicinal Chemistry | Year: 2016
Menadione (Vitamin K3) comprises of 1,4-naphthoquinone (NQ) moiety that can form redox isomers such as napthosemiquinone (NSQ) and catechol by accepting one or two electrons, respectively. The quinone redox cycling ability leads to the generation of "reactive oxygen species" (ROS) as well as arylation reactions, which are of biological relevance. This ability can be modulated with the help of suitable derivatization. A pharmacophore can be appended at suitable position of Vitamin K3 to have a synergistic or additive effect. In the present review, an attempt has been made to accrue such derivatives modified at 1 or 2 position and evaluated for their cytotoxicity activity on different series of human cancer cell lines such as HeLa, HL-60 and MCF- 7 etc. Production of reactive oxygen species (ROS) and mitochondrial dysfunction caused by Vitamin K3 derivatives leads to apoptosis and tumor inhibition. Recently, the CR-108 compound has shown to exhibit oxidative path together with non-oxidative phosphorylation of p38 MAP kinase in human breast cancer cells. Thus the chemical-biological interactions have been discussed which can be further extrapolated for the development of a potent anticancer drug. © 2016 Bentham Science Publishers.
Modak N.,MES Abasaheb Garware College |
Padhye A.,MES Abasaheb Garware College |
Dahanukar N.,Indian Institute of Science |
Zootaxa | Year: 2014
Indirana leithii (Boulenger, 1888) (Anura: Ranixalidae) is a frog species endemic to the Western Ghats and is categorized as Vulnerable according to IUCN red list. This species is currently considered to be widespread over the entire Western Ghats. Our study based on molecular data (using DNA sequence fragments of the mitochondrial 12S rRNA and 16S rRNA genes and the nuclear rhodopsin gene), morphological analysis of topotypic material as well as material collected from a wide range within the northern Western Ghats, suggests that the species has instead a restricted range in the state of Maharashtra. Specimens identified as I. leithii from the southern Western Ghats as well as from outside the Western Ghats probably belong to hitherto undescribed species. To facilitate future studies in understanding the nature of this species complex and provide better means for identification and delimitation of species we provide molecular, morphological and osteological characters of I. leithii from topotyic material. Copyright © 2014 Magnolia Press.
Nagare A.S.,CSIR - National Chemical Laboratory |
Manna A.,CSIR - National Chemical Laboratory |
Sonawane P.D.,MES Abasaheb Garware College |
Kumar A.,CSIR - National Chemical Laboratory
Journal of Physical Organic Chemistry | Year: 2015
Prevailing classification of salts based on their effect in solubility and stability of proteins in aqueous solution predicts that tetraalkylammonium salts, guanidinium chloride (GnCl), LiClO4 act as salting-in (S/I) and LiCl, NaCl act as salting-out (S/O) in aqueous conditions. In the same context the behaviour of GnCl, LiClO4 and LiCl are contradictory in polar solvents like ethylene glycol and formamide. In these solvents, expected salt effect shows just opposite nature from their usual expectation. However, in the aqueous solution salts like tetraalkylammonium halide (R4NX, R=alkyl group, X=Br group) behave like salting-in salts. The physicochemical origin of the salting in effect of R4NX type of salts has been discussed elaborately in the present work. The role of cations in terms of substitution of various alkyl groups on R4NX has been systematically presented here on the basis of experimental kinetic and thermodynamic studies. The abnormal behaviour of R4NX salts in aqueous solution has also been explained by the Setschenov equation (ks) and Δμsolvation values, which highlights their individual nature out of common properties of R4NX. © 2015 John Wiley & Sons, Ltd.
Nagpal V.,Agharkar Research Institute |
Bokare A.D.,Agharkar Research Institute |
Chikate R.C.,MES Abasaheb Garware College |
Rode C.V.,CSIR - National Chemical Laboratory |
Paknikar K.M.,Agharkar Research Institute
Journal of Hazardous Materials | Year: 2010
Nanoscale Fe-Pd bimetallic particles were synthesized and used for degradation of lindane (γ-hexachlorocyclohexane) in aqueous solution. Batch studies showed that 5 mg/L of lindane was completely dechlorinated within 5 min at a catalyst loading of 0.5 g/L and the degradation process followed first-order kinetics. GC-MS analysis in corroboration with GC-ECD results showed the presence of cyclohexane as the final degradation product. The proposed mechanism for the reductive dechlorination of lindane involves Fe corrosion-induced hydrogen atom transfer from the Pd surface. The enhanced degradation efficiency of Fe-Pd nanoparticles is attributed to: (1) high specific surface area of the nanoscale metal particles (60 m2/g), manyfold greater that of commercial grade micro- or milli-scale iron particles (∼1.6 m2/g); and, (2) increased catalytic reactivity due to the presence of Pd on the surface. Recycling and column studies showed that these nanoparticles exhibit efficient and sustained catalytic activity. © 2009 Elsevier B.V. All rights reserved.
Chikate R.C.,Post graduate and Research Center |
Kadu B.S.,Post graduate and Research Center |
Damle M.A.,MES Abasaheb Garware College
RSC Advances | Year: 2014
In this paper, we have developed an efficient electrostatic self-assembly strategy for the synthesis of a CdSe-CTAB-MMT composite that contains well-dispersed CdSe quantum dots (CdSe QDs) within the MMT structure. These nanocomposites are characterised by XRD, TEM, Raman, DRS and photoluminescence studies which indicate that 3-5 nm sized CdSe QDs are intercalated within lamellar sheets of MMT. Visible light induced photocatalytic activities of the composites exhibit decolourisation of Indigo Carmine (IC) solution (100 mg L-1) within 30 min. at 1.0 g L-1 catalyst loading. This feature is attributed to separation of photogenerated electron-hole pairs, enhanced interlayer spacing (7.2 A°), higher specific surface area and better adsorption capacity of the MMT. The involvement of reactive oxygen species (ROS) in the photodegradation process is ascertained by addition of selective quenchers such as NaN3 (for singlet oxygen), benzoquinone (for O2 -), ascorbic acid (for OH) and KI (for h +). It is observed that singlet oxygen and photogenerated h + do not contribute towards degradation; rather O2 - is a prominent species that degrades ∼74% of IC while the remaining part is oxidized by OH. The photodegradation pathway involves desulphonation of IC followed by its oxidation to isatin, anthranilic acid, tryptanthrin and isatoic anhydride. The antibacterial studies of degraded IC solution as well as chemoinformatics studies suggest that these metabolites are non-toxic in nature. These catalysts remain active for up to 6 cycles with a marginal decrease in their removal capacity that can be ascribed to inhibition of photocorrosion even after successive exposure to light. Thus, nano-engineered CdSe-composites may be regarded as efficient photocatalysts that have potential applications in sustainable development towards continuous removal of organic dyes from aqueous streams. This journal is © the Partner Organisations 2014.