São Cristóvão, Brazil
São Cristóvão, Brazil

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Kottwitz L.B.M.,West Parana State University | Scheffer M.C.,Federal University of Paraná | Costa L.M.D.,Federal University of Paraná | Leao J.A.,Mercolab Laboratorios Ltda | And 4 more authors.
Semina:Ciencias Agrarias | Year: 2012

Salmonella enterica subsp. enterica serovar Enteritidis (SE) is currently responsible for significant losses in the poultry industry with consequences on public health. In the present study, 38 SE isolates from biological material of chicken breeders were characterized using phage typing, antimicrobial resistance profile and pulsed field gel electrophoresis (PFGE). The phage type (PT) 7 and 9 were predominant and 26.3% of the isolates were resistance to nalidixic acid (NAL). The phage typing and analysis of antimicrobial resistance profile showed high discriminatory power (0.85). The PFGE profile of 13 strains with XbaI and SpeI discriminated the SE phage types, as well characterized strains from the same serovar. The results showed the importance to associate different methods for the characterization of SE and suggest that poultry products may have been source of human salmonellosis in the Parana State during the period of study.

de Freitas E.S.,MercoLab Laboratorios Ltda | Solda A.,MercoLab Laboratorios Ltda | Calegari D.L.,MercoLab Laboratorios Ltda | Munaretto F.C.,MercoLab Laboratorios Ltda | And 2 more authors.
Revista Brasileira de Ciencia Avicola | Year: 2014

Serum is widely used for the purpose of monitoring and diagnosis support for most of poultry diseases. In the case of the serum plate agglutination test (SPA), commonly used to detect antibodies for Salmonella Pullorum (SP), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS), serum cannot be frozen because it may result in false positive. Without freezing, serum can last only for a few days. In this experiment, glycerin was evaluated as a serum preservering agent. About 50 samples for each disease and analyzed by SPA test previously were separated. Glycerin was added to serum from commercial chickens, with and without antibodies for S P, MG and MS, in the proportion of 1:1 (serum:glycerin) and kept at refrigerated conditions (2 to 8˚C). For four years they were tested by the SPA, initially weekly, afterward monthly and then annually. The results show that serum with glycerin give consistent and valid results according to the kind of antibodies present for the period tested. Sera that glycerin was not added to, the results were valid only for the first week. From the second week on, microbial growth affected the test results of the sera without glycerin. Our investigation shows that glycerin can be used to preserve chicken serum for SPA under refrigerated conditions. It is an easy, simple and cheap procedure that can extend serum shelf life, useful mainly for control sera. © 2014, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.

Freitas E.S.,MercoLab Laboratorios Ltda | Back A.,MercoLab Laboratorios Ltda
Revista Brasileira de Ciencia Avicola | Year: 2015

Avian encephalomyelitis is caused by an Hepatovirus and primarily affects chickens. Chickens of all ages are susceptible to the virus, but the nervous symptoms are manifested only in young chicks, between one to five weeks of age. During the last thirty years, avian encephalomyelitis appeared to be well controlled by breeder vaccination. However, the increase of the number of cases is causing concern in the poultry industry. In the present study, we performed a retrospective analysis of the cases presenting histological lesions compatible with avian encephalomyelitis in broilers. The evaluated cases affected broilers from one to 35 days old from the southern region of Brazil. Only cases with compatible microscopic lesions and associated with clinical symptoms in the field were considered. In addition the histopathological diagnosis, sera were tested by ELISA (Enzyme-Linked Immunosorbent Assay). Considering the clinical, histopathological, and serological evidences, the disease was confirmed, showing an increase in outbreaks from the last quarter of 2012, extending through 2013. The cause of this increase is not clear, although we suspect vaccine or vaccination error. Enhancing vaccinated breeders flocks monitoring before the beginning of egg production and/or using a protocol with two vaccinations is recommended. © 2015, Fundacao APINCO de Ciencia e Tecnologia Avicolas. All rights reserved.

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